Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vapBC/- |
| Location | 58588..59282 | Replicon | plasmid pTT27 |
| Accession | NC_005838 | ||
| Organism | Thermus thermophilus HB27 | ||
Toxin (Protein)
| Gene name | vapC | Uniprot ID | - |
| Locus tag | TT_RS10465 | Protein ID | WP_162467455.1 |
| Coordinates | 58866..59282 (+) | Length | 139 a.a. |
Antitoxin (Protein)
| Gene name | vapB | Uniprot ID | - |
| Locus tag | TT_RS10460 | Protein ID | WP_162467454.1 |
| Coordinates | 58588..58869 (+) | Length | 94 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| TT_RS10435 (TT_P0059) | 53952..55121 | + | 1170 | WP_011174500.1 | cytochrome P450 | - |
| TT_RS10440 (TT_P0060) | 55118..55816 | + | 699 | WP_011174499.1 | lycopene cyclase domain-containing protein | - |
| TT_RS10445 (TT_P0061) | 55821..56498 | + | 678 | WP_011174498.1 | 1-acyl-sn-glycerol-3-phosphate acyltransferase | - |
| TT_RS10450 (TT_P0062) | 56495..57517 | + | 1023 | WP_011174497.1 | glycosyltransferase | - |
| TT_RS10455 (TT_P0063) | 57605..58294 | - | 690 | WP_008630619.1 | IS5-like element ISTth7 family transposase | - |
| TT_RS10460 (TT_P0064) | 58588..58869 | + | 282 | WP_162467454.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
| TT_RS10465 (TT_P0065) | 58866..59282 | + | 417 | WP_162467455.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| TT_RS10470 (TT_P0066) | 59380..60948 | + | 1569 | WP_041443894.1 | phytoene desaturase | - |
| TT_RS10475 (TT_P0067) | 60945..61943 | + | 999 | WP_011174493.1 | type 2 isopentenyl-diphosphate Delta-isomerase | - |
| TT_RS10480 (TT_P0068) | 61953..62345 | + | 393 | WP_011174492.1 | nucleotidyltransferase domain-containing protein | - |
| TT_RS10485 (TT_P0069) | 62376..63323 | - | 948 | WP_011174491.1 | DUF523 and DUF1722 domain-containing protein | - |
| TT_RS10490 (TT_P0070) | 63320..64108 | - | 789 | WP_011174490.1 | tryptophan-rich sensory protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..232605 | 232605 | |
| - | flank | IS/Tn | - | - | 57605..58090 | 485 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 139 a.a. Molecular weight: 10611.21 Da Isoelectric Point: 7.9719
>T10033 WP_162467454.1 NC_005838:58866-59282 [Thermus thermophilus HB27]
MRYLLDTNVVSEAVKRRPHPKVVAFLRHLKVGEAYLSALTLGELVQGVARAPEARRAILSRWLSGLKASFSGRILPLDAE
VMELWGELTGNAMREGKPLSPLDAMLAATALRHGLILATRNTEEFRHVPVPLVNPWEG
MRYLLDTNVVSEAVKRRPHPKVVAFLRHLKVGEAYLSALTLGELVQGVARAPEARRAILSRWLSGLKASFSGRILPLDAE
VMELWGELTGNAMREGKPLSPLDAMLAATALRHGLILATRNTEEFRHVPVPLVNPWEG
Download Length: 417 bp
>T10033 NC_005838:58866-59282 [Thermus thermophilus HB27]
CTGAGGTACCTGCTGGACACCAACGTGGTCTCCGAGGCCGTCAAGCGGCGGCCTCACCCCAAGGTGGTGGCCTTCCTGCG
CCACCTTAAAGTGGGGGAGGCGTACCTCTCCGCCCTCACCTTGGGCGAACTGGTCCAGGGGGTGGCCCGGGCCCCGGAGG
CCCGAAGGGCCATCCTCAGCCGATGGCTCTCGGGGCTGAAGGCGAGCTTCTCGGGGCGGATCCTTCCCCTGGACGCCGAG
GTGATGGAGCTCTGGGGCGAGCTCACCGGAAACGCCATGAGGGAGGGCAAGCCCCTCTCTCCCCTAGACGCCATGCTGGC
GGCCACGGCCCTGCGGCACGGCCTGATCCTGGCCACCCGGAACACCGAGGAGTTCCGCCACGTTCCCGTGCCCCTCGTGA
ACCCCTGGGAAGGGTAA
CTGAGGTACCTGCTGGACACCAACGTGGTCTCCGAGGCCGTCAAGCGGCGGCCTCACCCCAAGGTGGTGGCCTTCCTGCG
CCACCTTAAAGTGGGGGAGGCGTACCTCTCCGCCCTCACCTTGGGCGAACTGGTCCAGGGGGTGGCCCGGGCCCCGGAGG
CCCGAAGGGCCATCCTCAGCCGATGGCTCTCGGGGCTGAAGGCGAGCTTCTCGGGGCGGATCCTTCCCCTGGACGCCGAG
GTGATGGAGCTCTGGGGCGAGCTCACCGGAAACGCCATGAGGGAGGGCAAGCCCCTCTCTCCCCTAGACGCCATGCTGGC
GGCCACGGCCCTGCGGCACGGCCTGATCCTGGCCACCCGGAACACCGAGGAGTTCCGCCACGTTCCCGTGCCCCTCGTGA
ACCCCTGGGAAGGGTAA
Antitoxin
Download Length: 94 a.a. Molecular weight: 15255.85 Da Isoelectric Point: 10.4393
>AT10033 WP_162467455.1 NC_005838:58588-58869 [Thermus thermophilus HB27]
IYTRPPDLCKASPLQEAKARFSEVVERALKGEPQLVLRRGKKAVVVLDWETYARLTGEEASVLEALRPPKTLPNEEVEAL
FQRQKTGFREVDF
IYTRPPDLCKASPLQEAKARFSEVVERALKGEPQLVLRRGKKAVVVLDWETYARLTGEEASVLEALRPPKTLPNEEVEAL
FQRQKTGFREVDF
Download Length: 282 bp
>AT10033 NC_005838:58588-58869 [Thermus thermophilus HB27]
ATATACACCCGTCCTCCTGACCTTTGCAAGGCAAGCCCACTCCAAGAGGCCAAAGCCCGCTTTTCCGAGGTGGTGGAGCG
GGCCCTCAAGGGGGAGCCCCAGCTCGTCCTCCGGCGGGGTAAGAAGGCGGTGGTGGTGCTGGACTGGGAGACCTACGCCC
GGCTCACCGGGGAAGAGGCCTCGGTCCTCGAGGCCCTAAGACCCCCCAAGACCCTCCCCAATGAGGAGGTGGAAGCCCTC
TTCCAAAGGCAAAAGACGGGCTTCCGGGAGGTGGACTTCTGA
ATATACACCCGTCCTCCTGACCTTTGCAAGGCAAGCCCACTCCAAGAGGCCAAAGCCCGCTTTTCCGAGGTGGTGGAGCG
GGCCCTCAAGGGGGAGCCCCAGCTCGTCCTCCGGCGGGGTAAGAAGGCGGTGGTGGTGCTGGACTGGGAGACCTACGCCC
GGCTCACCGGGGAAGAGGCCTCGGTCCTCGAGGCCCTAAGACCCCCCAAGACCCTCCCCAATGAGGAGGTGGAAGCCCTC
TTCCAAAGGCAAAAGACGGGCTTCCGGGAGGTGGACTTCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10034 | Thermus thermophilus HB27 |
73.723 |
99.275 |
0.732 |
| T843 | Bradyrhizobium diazoefficiens USDA 110 |
43.165 |
100 |
0.435 |
| T6113 | Synechocystis sp. PCC 6803 |
37.063 |
100 |
0.384 |
| T10054 | Bosea sp. PAMC 26642 strain PAMC26642 |
33.571 |
100 |
0.356 |
| T10138 | Pseudomonas savastanoi |
33.088 |
98.551 |
0.326 |
| T4914 | Actinobacillus pleuropneumoniae serovar 3 str. JL03 |
31.111 |
100 |
0.326 |
| T6112 | uncultured bacterium |
30.07 |
100 |
0.323 |
| T4923 | Actinobacillus pleuropneumoniae serovar 3 str. JL03 |
29.655 |
100 |
0.312 |
| T4922 | Actinobacillus pleuropneumoniae serovar 3 str. JL03 |
29.71 |
100 |
0.311 |
| T1070 | Haemophilus influenzae Rd KW20 |
29.71 |
100 |
0.311 |
| T6111 | uncultured bacterium |
29.078 |
100 |
0.308 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT10034 | Thermus thermophilus HB27 |
70 |
93.023 |
0.651 |
Multiple sequence alignment
Structures
Toxin
| Source | ID | Structure |
|---|
Antitoxin
| Source | ID | Structure |
|---|
References
(1) Haijuan Li et al. (2023) The parABSm system is involved in megaplasmid partitioning and genome integrity maintenance in Thermus thermophilus. G3 (Bethesda, Md.) 13(4):jkad038. [PubMed:36786449]