4923

Detailed information of TA system    

experimentalExperimentally validated    

Overview


TA module


Type II Classification (family/domain) vapBC/PHD-PIN
Location 2013440..2014155 Replicon chromosome
Accession NC_010278
Organism Actinobacillus pleuropneumoniae serovar 3 str. JL03

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag APJL_1864 Protein ID WP_012263404.1
Coordinates 2013440..2013868 (-) Length 143 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID B0BT01
Locus tag APJL_1865 Protein ID WP_005599447.1
Coordinates 2013868..2014155 (-) Length 96 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
APJL_RS09410 (APJL_1861) 2009025..2010731 - 1707 WP_005606484.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
APJL_RS09415 (APJL_1862) 2010827..2011108 - 282 WP_012263402.1 DNA-directed RNA polymerase subunit omega -
APJL_RS09420 (APJL_1863) 2011235..2013316 - 2082 WP_012263403.1 ATP-dependent DNA helicase RecG -
APJL_RS09425 (APJL_1864) 2013440..2013868 - 429 WP_012263404.1 type II toxin-antitoxin system VapC family toxin Toxin
APJL_RS09430 (APJL_1865) 2013868..2014155 - 288 WP_005599447.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
APJL_RS09435 (APJL_1867) 2014361..2016076 + 1716 WP_012263406.1 proline--tRNA ligase -
APJL_RS09440 (APJL_1868) 2016142..2016330 - 189 WP_005602684.1 hypothetical protein -
APJL_RS09445 (APJL_1869) 2016333..2016914 - 582 WP_012263407.1 sigma-70 family RNA polymerase sigma factor -
APJL_RS09450 (APJL_1870) 2017003..2017959 + 957 WP_012263408.1 protein-methionine-sulfoxide reductase catalytic subunit MsrP -
APJL_RS09455 (APJL_1871) 2017959..2018552 + 594 WP_005599453.1 sulfoxide reductase heme-binding subunit YedZ -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-124)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 143 a.a.        Molecular weight: 16495.92 Da        Isoelectric Point: 7.7945

>T4923 WP_012263404.1 NC_010278:c2013868-2013440 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
MFQYLFDTNIISELYKLGNSRIDTNVRQWLETIKPSQTNISCITLSEIKTGILLKARKDPIQAERLNHWFTHNVLSVYQA
KSFSINNEIALLASEYHIPNKMDLNDAYIAATAKYHNLVLVTRNLKDFNCCDIRLFNPFEPN

Download         Length: 429 bp

>T4923 NC_010278:c2013868-2013440 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
ATGTTTCAATATCTTTTTGATACCAACATCATTTCCGAGCTTTATAAGCTAGGAAACAGTAGAATAGATACCAATGTACG
ACAATGGTTAGAAACGATTAAACCGAGTCAGACGAATATTAGCTGTATCACGCTTTCCGAAATAAAAACGGGGATTTTAC
TTAAAGCTCGTAAAGATCCTATTCAAGCCGAGCGGTTAAATCATTGGTTTACCCATAATGTATTAAGCGTTTATCAAGCT
AAATCCTTTTCTATAAATAATGAAATTGCGTTACTGGCTTCTGAATACCACATTCCAAATAAAATGGATTTAAACGATGC
TTATATTGCCGCAACCGCTAAATATCATAATTTAGTGCTTGTTACTCGTAATTTGAAAGACTTCAACTGTTGCGATATAC
GTTTATTTAATCCTTTCGAACCGAATTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 11040.29 Da        Isoelectric Point: 4.5578

>AT4923 WP_005599447.1 NC_010278:c2014155-2013868 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
MPITITSREFNQHASKVLKMAEKEPVFITKWGKIVSVVSNYVDYKQHTTVEPSFEELFASSTDESVSDEFWQDFEQTLTK
IRKSNKSEQIDFGDE

Download         Length: 288 bp

>AT4923 NC_010278:c2014155-2013868 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
ATGCCAATTACTATTACCTCTCGAGAATTTAATCAACACGCCAGTAAAGTGCTGAAAATGGCAGAAAAAGAACCGGTTTT
TATCACTAAATGGGGAAAAATTGTCAGCGTTGTCTCCAATTATGTTGATTACAAACAGCACACAACGGTTGAGCCTAGCT
TTGAAGAACTATTTGCCTCATCTACGGATGAATCGGTTAGCGATGAGTTTTGGCAAGATTTTGAACAGACATTAACAAAA
ATTCGCAAAAGTAATAAATCCGAACAAATAGATTTTGGAGATGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T4250 Klebsiella pneumoniae subsp. pneumoniae MGH 78578

33.088

100

0.354

T6113 Synechocystis sp. PCC 6803

35.461

99.296

0.352

T10034 Thermus thermophilus HB27

31.25

100

0.326

T843 Bradyrhizobium diazoefficiens USDA 110

31.206

100

0.312


Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT10033 Thermus thermophilus HB27

29.655

100

0.312


Multiple sequence alignment    




Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB A3N3C5

References


(1) Chengkun Zheng et al. (2017) Identification of four type II toxin-antitoxin systems in Actinobacillus pleuropneumoniae. FEMS Microbiology Letters 364(12):10.1093/femsle/fnx126. [PubMed:28637172] experimental


experimental experimental literature