Detailed information of TA system
Experimentally validatedOverview
TA module
Type | II | Classification (family/domain) | vapBC/PHD-PIN |
Location | 2013440..2014155 | Replicon | chromosome |
Accession | NC_010278 | ||
Organism | Actinobacillus pleuropneumoniae serovar 3 str. JL03 |
Toxin (Protein)
Gene name | vapC | Uniprot ID | - |
Locus tag | APJL_1864 | Protein ID | WP_012263404.1 |
Coordinates | 2013440..2013868 (-) | Length | 143 a.a. |
Antitoxin (Protein)
Gene name | vapB | Uniprot ID | B0BT01 |
Locus tag | APJL_1865 | Protein ID | WP_005599447.1 |
Coordinates | 2013868..2014155 (-) | Length | 96 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
APJL_RS09410 (APJL_1861) | 2009025..2010731 | - | 1707 | WP_005606484.1 | bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase | - |
APJL_RS09415 (APJL_1862) | 2010827..2011108 | - | 282 | WP_012263402.1 | DNA-directed RNA polymerase subunit omega | - |
APJL_RS09420 (APJL_1863) | 2011235..2013316 | - | 2082 | WP_012263403.1 | ATP-dependent DNA helicase RecG | - |
APJL_RS09425 (APJL_1864) | 2013440..2013868 | - | 429 | WP_012263404.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
APJL_RS09430 (APJL_1865) | 2013868..2014155 | - | 288 | WP_005599447.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
APJL_RS09435 (APJL_1867) | 2014361..2016076 | + | 1716 | WP_012263406.1 | proline--tRNA ligase | - |
APJL_RS09440 (APJL_1868) | 2016142..2016330 | - | 189 | WP_005602684.1 | hypothetical protein | - |
APJL_RS09445 (APJL_1869) | 2016333..2016914 | - | 582 | WP_012263407.1 | sigma-70 family RNA polymerase sigma factor | - |
APJL_RS09450 (APJL_1870) | 2017003..2017959 | + | 957 | WP_012263408.1 | protein-methionine-sulfoxide reductase catalytic subunit MsrP | - |
APJL_RS09455 (APJL_1871) | 2017959..2018552 | + | 594 | WP_005599453.1 | sulfoxide reductase heme-binding subunit YedZ | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 143 a.a. Molecular weight: 16495.92 Da Isoelectric Point: 7.7945
>T4923 WP_012263404.1 NC_010278:c2013868-2013440 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
MFQYLFDTNIISELYKLGNSRIDTNVRQWLETIKPSQTNISCITLSEIKTGILLKARKDPIQAERLNHWFTHNVLSVYQA
KSFSINNEIALLASEYHIPNKMDLNDAYIAATAKYHNLVLVTRNLKDFNCCDIRLFNPFEPN
MFQYLFDTNIISELYKLGNSRIDTNVRQWLETIKPSQTNISCITLSEIKTGILLKARKDPIQAERLNHWFTHNVLSVYQA
KSFSINNEIALLASEYHIPNKMDLNDAYIAATAKYHNLVLVTRNLKDFNCCDIRLFNPFEPN
Download Length: 429 bp
>T4923 NC_010278:c2013868-2013440 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
ATGTTTCAATATCTTTTTGATACCAACATCATTTCCGAGCTTTATAAGCTAGGAAACAGTAGAATAGATACCAATGTACG
ACAATGGTTAGAAACGATTAAACCGAGTCAGACGAATATTAGCTGTATCACGCTTTCCGAAATAAAAACGGGGATTTTAC
TTAAAGCTCGTAAAGATCCTATTCAAGCCGAGCGGTTAAATCATTGGTTTACCCATAATGTATTAAGCGTTTATCAAGCT
AAATCCTTTTCTATAAATAATGAAATTGCGTTACTGGCTTCTGAATACCACATTCCAAATAAAATGGATTTAAACGATGC
TTATATTGCCGCAACCGCTAAATATCATAATTTAGTGCTTGTTACTCGTAATTTGAAAGACTTCAACTGTTGCGATATAC
GTTTATTTAATCCTTTCGAACCGAATTAA
ATGTTTCAATATCTTTTTGATACCAACATCATTTCCGAGCTTTATAAGCTAGGAAACAGTAGAATAGATACCAATGTACG
ACAATGGTTAGAAACGATTAAACCGAGTCAGACGAATATTAGCTGTATCACGCTTTCCGAAATAAAAACGGGGATTTTAC
TTAAAGCTCGTAAAGATCCTATTCAAGCCGAGCGGTTAAATCATTGGTTTACCCATAATGTATTAAGCGTTTATCAAGCT
AAATCCTTTTCTATAAATAATGAAATTGCGTTACTGGCTTCTGAATACCACATTCCAAATAAAATGGATTTAAACGATGC
TTATATTGCCGCAACCGCTAAATATCATAATTTAGTGCTTGTTACTCGTAATTTGAAAGACTTCAACTGTTGCGATATAC
GTTTATTTAATCCTTTCGAACCGAATTAA
Antitoxin
Download Length: 96 a.a. Molecular weight: 11040.29 Da Isoelectric Point: 4.5578
>AT4923 WP_005599447.1 NC_010278:c2014155-2013868 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
MPITITSREFNQHASKVLKMAEKEPVFITKWGKIVSVVSNYVDYKQHTTVEPSFEELFASSTDESVSDEFWQDFEQTLTK
IRKSNKSEQIDFGDE
MPITITSREFNQHASKVLKMAEKEPVFITKWGKIVSVVSNYVDYKQHTTVEPSFEELFASSTDESVSDEFWQDFEQTLTK
IRKSNKSEQIDFGDE
Download Length: 288 bp
>AT4923 NC_010278:c2014155-2013868 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
ATGCCAATTACTATTACCTCTCGAGAATTTAATCAACACGCCAGTAAAGTGCTGAAAATGGCAGAAAAAGAACCGGTTTT
TATCACTAAATGGGGAAAAATTGTCAGCGTTGTCTCCAATTATGTTGATTACAAACAGCACACAACGGTTGAGCCTAGCT
TTGAAGAACTATTTGCCTCATCTACGGATGAATCGGTTAGCGATGAGTTTTGGCAAGATTTTGAACAGACATTAACAAAA
ATTCGCAAAAGTAATAAATCCGAACAAATAGATTTTGGAGATGAATAA
ATGCCAATTACTATTACCTCTCGAGAATTTAATCAACACGCCAGTAAAGTGCTGAAAATGGCAGAAAAAGAACCGGTTTT
TATCACTAAATGGGGAAAAATTGTCAGCGTTGTCTCCAATTATGTTGATTACAAACAGCACACAACGGTTGAGCCTAGCT
TTGAAGAACTATTTGCCTCATCTACGGATGAATCGGTTAGCGATGAGTTTTGGCAAGATTTTGAACAGACATTAACAAAA
ATTCGCAAAAGTAATAAATCCGAACAAATAGATTTTGGAGATGAATAA
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|---|---|---|---|
T4250 | Klebsiella pneumoniae subsp. pneumoniae MGH 78578 |
33.088 |
100 |
0.354 |
T6113 | Synechocystis sp. PCC 6803 |
35.461 |
99.296 |
0.352 |
T10034 | Thermus thermophilus HB27 |
31.25 |
100 |
0.326 |
T843 | Bradyrhizobium diazoefficiens USDA 110 |
31.206 |
100 |
0.312 |
Multiple sequence alignment
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|---|---|---|---|
AT10033 | Thermus thermophilus HB27 |
29.655 |
100 |
0.312 |
Multiple sequence alignment
References
(1) Chengkun Zheng et al. (2017) Identification of four type II toxin-antitoxin systems in Actinobacillus pleuropneumoniae. FEMS Microbiology Letters 364(12):10.1093/femsle/fnx126. [PubMed:28637172]
experimental literature