Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vapBC/RHH-PIN |
| Location | 4585664..4586295 | Replicon | chromosome |
| Accession | NC_009648 | ||
| Organism | Klebsiella pneumoniae subsp. pneumoniae MGH 78578 | ||
Toxin (Protein)
| Gene name | vapC | Uniprot ID | - |
| Locus tag | KPN_04186 | Protein ID | 152972686 |
| Coordinates | 4585912..4586295 (+) | Length | 128 a.a. |
Antitoxin (Protein)
| Gene name | vapB | Uniprot ID | - |
| Locus tag | KPN_04185 | Protein ID | 152972685 |
| Coordinates | 4585670..4585912 (+) | Length | 81 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KPN_RS22540 (KPN_04180) | 4581661..4582260 | + | 600 | WP_004151865.1 | glucose-1-phosphatase | - |
| KPN_RS22545 (KPN_04181) | 4582254..4583114 | + | 861 | WP_004146232.1 | virulence factor BrkB family protein | - |
| KPN_RS22550 (KPN_04182) | 4583111..4583548 | + | 438 | WP_002882809.1 | D-tyrosyl-tRNA(Tyr) deacylase | - |
| KPN_RS22555 (KPN_04183) | 4583593..4584534 | + | 942 | WP_004178031.1 | fatty acid biosynthesis protein FabY | - |
| KPN_RS22560 (KPN_04184) | 4584548..4585465 | - | 918 | WP_015959199.1 | alpha/beta hydrolase | - |
| KPN_RS22565 (KPN_04185) | 4585670..4585912 | + | 243 | WP_004150355.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| KPN_RS22570 (KPN_04186) | 4585912..4586295 | + | 384 | WP_002882817.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| KPN_RS22575 (KPN_04187) | 4586469..4587398 | - | 930 | WP_004150358.1 | formate dehydrogenase accessory protein FdhE | - |
| KPN_RS22580 (KPN_04188) | 4587395..4588030 | - | 636 | WP_002882818.1 | formate dehydrogenase cytochrome b556 subunit | - |
| KPN_RS22585 (KPN_04189) | 4588027..4588929 | - | 903 | WP_002882822.1 | formate dehydrogenase subunit beta | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 128 a.a. Molecular weight: 14377.62 Da Isoelectric Point: 7.3178
>T4250 152972686 NC_009648:4585912-4586295 [Klebsiella pneumoniae subsp. pneumoniae MGH 78578]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE
Download Length: 384 bp
>T4250 NC_009648:4585912-4586295 [Klebsiella pneumoniae subsp. pneumoniae MGH 78578]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA
Antitoxin
Download Length: 81 a.a. Molecular weight: 9567.83 Da Isoelectric Point: 4.3509
>AT4250 152972685 NC_009648:4585670-4585912 [Klebsiella pneumoniae subsp. pneumoniae MGH 78578]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW
Download Length: 243 bp
>AT4250 NC_009648:4585670-4585912 [Klebsiella pneumoniae subsp. pneumoniae MGH 78578]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T4923 | Actinobacillus pleuropneumoniae serovar 3 str. JL03 |
33.088 |
100 |
0.354 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|
Antitoxin
| Source | ID | Structure |
|---|
References
(1) Sung-Min Kang et al. (2020) Structural and Functional Study of the Klebsiella pneumoniae VapBC Toxin-Antitoxin System, Including the Development of an Inhibitor That Activates VapC. Journal of Medicinal Chemistry 63(22):13669-13679. [PubMed:33146528]