26989

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-VagC
Location 1007315..1007943 Replicon chromosome
Accession NC_017551
Organism Leptospira interrogans serovar Lai str. IPAV

Toxin (Protein)


Gene name vapC Uniprot ID Q8F7E4
Locus tag LIF_RS04330 Protein ID WP_000281042.1
Coordinates 1007315..1007713 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q8F7E3
Locus tag LIF_RS04335 Protein ID WP_001192482.1
Coordinates 1007713..1007943 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LIF_RS04305 1002607..1003374 + 768 WP_000636952.1 DUF1554 domain-containing protein -
LIF_RS04310 1003915..1004775 + 861 WP_000682233.1 hypothetical protein -
LIF_RS04315 1004992..1005450 - 459 WP_001239004.1 GNAT family N-acetyltransferase -
LIF_RS04320 1005451..1006527 - 1077 WP_001170569.1 hypothetical protein -
LIF_RS04325 1006669..1007217 - 549 WP_000479564.1 hypothetical protein -
LIF_RS04330 1007315..1007713 - 399 WP_000281042.1 type II toxin-antitoxin system VapC family toxin Toxin
LIF_RS04335 1007713..1007943 - 231 WP_001192482.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
LIF_RS04340 1008191..1008868 - 678 WP_000071998.1 hypothetical protein -
LIF_RS04345 1009100..1009789 - 690 WP_000590463.1 SH3 domain-containing protein -
LIF_RS04350 1010015..1010638 - 624 Protein_828 M20/M25/M40 family metallo-hydrolase -
LIF_RS04355 1010888..1011961 + 1074 WP_000445690.1 IS110-like element ISLin1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 1010888..1011961 1073


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-123)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15139.76 Da        Isoelectric Point: 9.6893

>T26989 WP_000281042.1 NC_017551:c1007713-1007315 [Leptospira interrogans serovar Lai str. IPAV]
MYLLDTNICIFLIKKKNATLLENLKKKLNKDLFVSSLTVAELEFGIQKSEFKEKNKVALIEFLTIFNILSFSDKDAESYG
IIRADLERKGNVIGSIDMLLAAQAIANNYIFVTNNTKEFKRIKALKIENWTQ

Download         Length: 399 bp

>T26989 NC_017551:c1007713-1007315 [Leptospira interrogans serovar Lai str. IPAV]
ATGTATCTTTTGGATACCAATATATGCATTTTTCTAATTAAAAAGAAAAATGCGACTCTGTTAGAAAATCTTAAGAAAAA
ATTAAATAAAGATCTATTTGTTTCGTCTTTAACTGTTGCGGAACTTGAGTTTGGAATTCAGAAAAGTGAATTTAAAGAAA
AGAATAAAGTTGCACTAATCGAATTTTTAACGATTTTCAATATACTTTCCTTTTCTGATAAAGATGCAGAGTCTTACGGT
ATCATCAGAGCGGATTTGGAGAGGAAAGGTAATGTAATCGGTTCAATAGACATGTTACTTGCCGCTCAAGCAATTGCTAA
TAATTATATCTTTGTTACTAATAATACAAAAGAATTTAAAAGAATTAAGGCTTTAAAAATAGAAAATTGGACACAGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8771.05 Da        Isoelectric Point: 8.6588

>AT26989 WP_001192482.1 NC_017551:c1007943-1007713 [Leptospira interrogans serovar Lai str. IPAV]
MQTAKLFINGRSQAVRLPKEFQFTGDDVLIQKVGEAVILVPKNKAWNVFLEGLNGFSDDFFKEEREQPKSDKREKL

Download         Length: 231 bp

>AT26989 NC_017551:c1007943-1007713 [Leptospira interrogans serovar Lai str. IPAV]
ATGCAAACAGCCAAATTATTTATAAATGGAAGAAGTCAAGCGGTTCGACTACCAAAAGAATTTCAATTTACCGGAGATGA
TGTTCTTATCCAAAAAGTTGGAGAGGCGGTAATATTGGTTCCGAAAAACAAGGCCTGGAACGTATTTTTAGAAGGATTGA
ACGGGTTTTCAGATGATTTCTTTAAGGAGGAGAGAGAACAGCCGAAATCTGATAAAAGAGAAAAGCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F6HAN6

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HAF7

References