Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) mazEF/AbrB-MazF
Location 417705..418294 Replicon chromosome
Accession NC_001263
Organism Deinococcus radiodurans R1

Toxin (Protein)


Gene name mazF Uniprot ID Q9RX98
Locus tag DR_RS02150 Protein ID WP_010887062.1
Coordinates 417941..418294 (+) Length 118 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID Q9RX99
Locus tag DR_RS02145 Protein ID WP_010887061.1
Coordinates 417705..417947 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DR_RS02110 (DR_0409) 412718..413137 + 420 WP_010887054.1 DinB family protein -
DR_RS02115 (DR_0410) 413130..413501 + 372 Protein_403 inorganic pyrophosphatase -
DR_RS02120 (DR_0411) 413498..414181 + 684 WP_010887056.1 SDR family oxidoreductase -
DR_RS02125 (DR_0412) 414202..415164 + 963 WP_034349507.1 NAD(P)/FAD-dependent oxidoreductase -
DR_RS02130 (DR_0413) 415187..415366 + 180 WP_034349506.1 hypothetical protein -
DR_RS02135 (DR_0414) 415368..415996 + 629 Protein_407 HAD family hydrolase -
DR_RS02140 (DR_0415) 416044..417525 - 1482 Protein_408 carboxylesterase/lipase family protein -
DR_RS02145 (DR_0416) 417705..417947 + 243 WP_010887061.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
DR_RS02150 (DR_0417) 417941..418294 + 354 WP_010887062.1 endoribonuclease MazF Toxin
DR_RS02155 (DR_0418) 418347..418571 + 225 WP_162177573.1 hypothetical protein -
DR_RS02160 (DR_0419) 418568..418981 + 414 WP_010887064.1 type II toxin-antitoxin system VapC family toxin -
DR_RS02165 (DR_0420) 418978..421446 + 2469 WP_010887065.1 ATP-dependent helicase HrpB -
DR_RS02170 (DR_0421) 421627..421851 - 225 WP_162177572.1 hypothetical protein -
DR_RS02175 (DR_0422) 422007..422783 - 777 WP_010887067.1 trans-aconitate 2-methyltransferase -
DR_RS02180 422904..423083 - 180 WP_028328008.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Antitoxin PemI-like

(1-80)


Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 12450.24 Da        Isoelectric Point: 7.3219

>T137 WP_010887062.1 NC_001263:417941-418294 [Deinococcus radiodurans R1]
MVSDYVPDAGHLVWLNFTPQAGHEQGGRRPALVLSPAAYNGVTGLMQACPVTSRAKGYPFEVTLPAHLGVSGVVLADHCR
SLDWRSRRAEQLAEAPADVLAEVRGKLGSLLGMSEKA

Download         Length: 354 bp

>T137 NC_001263:417941-418294 [Deinococcus radiodurans R1]
ATGGTAAGCGATTATGTCCCGGACGCTGGGCATCTGGTGTGGCTCAACTTCACACCTCAGGCAGGGCATGAACAGGGGGG
ACGGCGACCGGCGCTGGTGCTTTCGCCTGCCGCTTATAACGGCGTCACTGGACTGATGCAGGCGTGTCCGGTGACCAGCC
GCGCCAAGGGGTATCCGTTTGAAGTGACGCTCCCTGCTCACCTTGGAGTCAGCGGCGTGGTGCTGGCCGACCATTGCCGC
AGCCTCGACTGGCGCAGTCGCCGGGCCGAACAACTTGCGGAGGCGCCGGCGGACGTCCTGGCCGAAGTCCGTGGCAAGTT
GGGCAGCTTGCTGGGCATGTCCGAGAAGGCATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8911.12 Da        Isoelectric Point: 4.3178

>AT137 WP_010887061.1 NC_001263:417705-417947 [Deinococcus radiodurans R1]
MTSQIQKWGNSLALRIPKALAQQVGLTQSSEVELLLQDGQIVIRPVPARQYDLAALLAEMTPENLHGETDWGALEGREEW

Download         Length: 243 bp

>AT137 NC_001263:417705-417947 [Deinococcus radiodurans R1]
ATGACGAGTCAAATTCAGAAATGGGGCAACAGCCTCGCGCTCCGCATTCCCAAAGCTCTGGCGCAGCAGGTGGGACTGAC
GCAGAGTTCAGAAGTGGAGCTGCTTCTTCAGGACGGTCAGATTGTCATCCGGCCAGTTCCTGCTCGGCAGTACGATCTCG
CCGCGCTGCTGGCCGAAATGACACCTGAAAATCTGCATGGGGAAACAGACTGGGGCGCACTGGAAGGACGCGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T1044 Escherichia coli str. K-12 substr. MG1655

45.536

100

0.459
T10130 Escherichia coli str. K-12 substr. MC4100 strain K-12

45.536

100

0.459
T10069 Klebsiella pneumoniae subsp. pneumoniae MGH 78578

40.909

98.214

0.402
T1076 Shigella flexneri 2b

39.423

88.889

0.35
T1049 Escherichia coli str. K-12 substr. MG1655

37.383

92.241

0.345
T10090 Enterococcus faecalis ATCC 29212

36.364

87.611

0.319
T6120 Xylella fastidiosa

34

92.593

0.315

Multiple sequence alignment    



Structures

Toxin

Source ID Structure
PDB 7D2M
PDB 7D2P
PDB 7DHP
PDB 7D2Q
PDB 7D28
PDB 7D2N

Antitoxin

Source ID Structure
PDB 7D2P
PDB 7D2Q
PDB 7D2N
AlphaFold DB Q9RX99

References

(1) Katsuhiko Kamada et al. (2003) Crystal structure of the MazE/MazF complex: molecular bases of antidote-toxin recognition. Molecular Cell 11(4):875-84. [PubMed:12718874] 3D_structure

(2) Tatsuki Miyamoto et al. (2017) Characterization of a Deinococcus radiodurans MazF: A UACA-specific RNA endoribonuclease. MicrobiologyOpen 6(5):e00501. [PubMed:28675659] experimental

(3) Tao Li et al. (2017) Deinococcus radiodurans Toxin-Antitoxin MazEF-dr Mediates Cell Death in Response to DNA Damage Stress. Frontiers in Microbiology 8:1427. [PubMed:28798741] experimental


experimental experimental literature
3D_structure protein structure literature