Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/MazF(toxin) |
| Location | 2404180..2404742 | Replicon | chromosome |
| Accession | NC_017491 | ||
| Organism | Lactobacillus rhamnosus ATCC 8530 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | C2JYM4 |
| Locus tag | LRHK_RS11050 | Protein ID | WP_005692155.1 |
| Coordinates | 2404180..2404527 (-) | Length | 116 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | - |
| Locus tag | LRHK_RS11055 | Protein ID | WP_014571589.1 |
| Coordinates | 2404527..2404742 (-) | Length | 72 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LRHK_RS11030 (LRHK_2449) | 2399675..2400916 | - | 1242 | WP_014571587.1 | acyltransferase | - |
| LRHK_RS11035 (LRHK_2450) | 2400913..2401614 | - | 702 | WP_005692159.1 | ABC transporter ATP-binding protein | - |
| LRHK_RS11040 (LRHK_2451) | 2401607..2402425 | - | 819 | WP_014571588.1 | ABC transporter permease subunit | - |
| LRHK_RS11045 (LRHK_2452) | 2402666..2403334 | - | 669 | WP_005692157.1 | tRNA ligase | - |
| LRHK_RS14705 | 2403514..2403657 | + | 144 | WP_015764720.1 | hypothetical protein | - |
| LRHK_RS11050 (LRHK_2454) | 2404180..2404527 | - | 348 | WP_005692155.1 | type II toxin-antitoxin system PemK/MazF family toxin | Toxin |
| LRHK_RS11055 (LRHK_2455) | 2404527..2404742 | - | 216 | WP_014571589.1 | hypothetical protein | Antitoxin |
| LRHK_RS11060 (LRHK_2456) | 2404839..2406674 | - | 1836 | WP_005692152.1 | ABC transporter ATP-binding protein/permease | - |
| LRHK_RS11065 (LRHK_2457) | 2406661..2408451 | - | 1791 | WP_005692150.1 | ABC transporter ATP-binding protein/permease | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 116 a.a. Molecular weight: 13150.01 Da Isoelectric Point: 7.9817
>T6238 WP_005692155.1 NC_017491:c2404527-2404180 [Lactobacillus rhamnosus ATCC 8530]
MTYKAKQGDVVWLDFDPSEGHEIRKRCPAVVLSSNAYNRATGFVIVAPITSTIRTLPGYVDIVSNKIRGQVVTSQVYSLD
VTEQGNRKIDFIERMNIEDFYTVAQFTKMNFDFKF
MTYKAKQGDVVWLDFDPSEGHEIRKRCPAVVLSSNAYNRATGFVIVAPITSTIRTLPGYVDIVSNKIRGQVVTSQVYSLD
VTEQGNRKIDFIERMNIEDFYTVAQFTKMNFDFKF
Download Length: 348 bp
>T6238 NC_017491:c2404527-2404180 [Lactobacillus rhamnosus ATCC 8530]
ATGACATATAAGGCTAAGCAAGGTGATGTGGTGTGGCTTGATTTTGATCCGTCCGAGGGTCATGAAATTAGAAAAAGATG
CCCAGCTGTTGTTTTAAGCAGTAACGCATACAATCGTGCAACCGGATTTGTGATTGTCGCACCAATTACCTCAACAATTC
GGACACTACCTGGTTATGTTGATATTGTATCCAATAAAATTCGTGGTCAGGTTGTCACTTCGCAGGTTTATTCTCTGGAT
GTCACTGAACAAGGCAACCGAAAAATCGATTTTATTGAAAGAATGAACATTGAAGATTTTTATACAGTCGCTCAATTCAC
CAAAATGAATTTTGATTTTAAATTTTAG
ATGACATATAAGGCTAAGCAAGGTGATGTGGTGTGGCTTGATTTTGATCCGTCCGAGGGTCATGAAATTAGAAAAAGATG
CCCAGCTGTTGTTTTAAGCAGTAACGCATACAATCGTGCAACCGGATTTGTGATTGTCGCACCAATTACCTCAACAATTC
GGACACTACCTGGTTATGTTGATATTGTATCCAATAAAATTCGTGGTCAGGTTGTCACTTCGCAGGTTTATTCTCTGGAT
GTCACTGAACAAGGCAACCGAAAAATCGATTTTATTGAAAGAATGAACATTGAAGATTTTTATACAGTCGCTCAATTCAC
CAAAATGAATTTTGATTTTAAATTTTAG
Antitoxin
Download Length: 72 a.a. Molecular weight: 7986.13 Da Isoelectric Point: 5.7995
>AT6238 WP_014571589.1 NC_017491:c2404742-2404527 [Lactobacillus rhamnosus ATCC 8530]
MDVKVVKRGNSLALSLPSSAGFKRGEAFLLISDEKGGYLLIPKVKNPYTKAKPLSFHQEEAWPDFDYEDIE
MDVKVVKRGNSLALSLPSSAGFKRGEAFLLISDEKGGYLLIPKVKNPYTKAKPLSFHQEEAWPDFDYEDIE
Download Length: 216 bp
>AT6238 NC_017491:c2404742-2404527 [Lactobacillus rhamnosus ATCC 8530]
ATGGATGTGAAAGTTGTAAAACGTGGAAACTCGTTAGCTTTATCACTTCCGAGCAGTGCGGGTTTCAAACGAGGCGAGGC
GTTTTTGCTGATTTCAGATGAAAAAGGTGGTTATCTGTTGATCCCAAAGGTAAAAAATCCCTATACCAAAGCAAAACCAT
TATCATTCCATCAAGAAGAAGCTTGGCCGGATTTTGACTATGAGGATATTGAATAA
ATGGATGTGAAAGTTGTAAAACGTGGAAACTCGTTAGCTTTATCACTTCCGAGCAGTGCGGGTTTCAAACGAGGCGAGGC
GTTTTTGCTGATTTCAGATGAAAAAGGTGGTTATCTGTTGATCCCAAAGGTAAAAAATCCCTATACCAAAGCAAAACCAT
TATCATTCCATCAAGAAGAAGCTTGGCCGGATTTTGACTATGAGGATATTGAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T6137 | Lactobacillus rhamnosus Lc 705 |
100 |
100 |
1 |
| T6142 | Lactobacillus rhamnosus ATCC 8530 |
100 |
100 |
1 |
| T10090 | Enterococcus faecalis ATCC 29212 |
42.727 |
97.345 |
0.416 |
| T10069 | Klebsiella pneumoniae subsp. pneumoniae MGH 78578 |
38.71 |
83.036 |
0.321 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT6137 | Lactobacillus rhamnosus Lc 705 |
100 |
100 |
1 |
| AT6142 | Lactobacillus rhamnosus ATCC 8530 |
100 |
100 |
1 |
| AT137 | Deinococcus radiodurans R1 |
30.137 |
100 |
0.31 |
Multiple sequence alignment
References
(1) K M Klimina et al. (2013) Identification and characterization of toxin-antitoxin systems in strains of Lactobacillus rhamnosus isolated from humans. Anaerobe 22:82-9. [PubMed:23727113]