Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/- |
| Location | 2000373..2000944 | Replicon | chromosome |
| Accession | NZ_CP008816 | ||
| Organism | Enterococcus faecalis ATCC 29212 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | - |
| Locus tag | DR75_RS10510 | Protein ID | WP_002354774.1 |
| Coordinates | 2000373..2000714 (-) | Length | 114 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | R3JGB1 |
| Locus tag | DR75_RS10515 | Protein ID | WP_002354773.1 |
| Coordinates | 2000714..2000944 (-) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| DR75_RS10505 (DR75_1947) | 1996388..2000002 | - | 3615 | WP_002389470.1 | DNA-directed RNA polymerase subunit beta | - |
| DR75_RS10510 (DR75_1948) | 2000373..2000714 | - | 342 | WP_002354774.1 | type II toxin-antitoxin system PemK/MazF family toxin | Toxin |
| DR75_RS10515 (DR75_1949) | 2000714..2000944 | - | 231 | WP_002354773.1 | hypothetical protein | Antitoxin |
| DR75_RS10520 (DR75_1950) | 2001119..2001334 | - | 216 | WP_002354768.1 | zinc ribbon domain-containing protein | - |
| DR75_RS10525 (DR75_1951) | 2001473..2002465 | + | 993 | WP_002354765.1 | biotin--[acetyl-CoA-carboxylase] ligase | - |
| DR75_RS10530 (DR75_1952) | 2002729..2003358 | - | 630 | WP_002354763.1 | lytic polysaccharide monooxygenase | - |
| DR75_RS10540 (DR75_1953) | 2004051..2005667 | + | 1617 | WP_002381036.1 | phosphatase PAP2/LCP family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| inside | Integrative and Conjugative Element | - | - | 1970908..2015454 | 44546 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 114 a.a. Molecular weight: 13156.40 Da Isoelectric Point: 9.3984
>T10090 WP_002354774.1 NZ_CP008816:c2000714-2000373 [Enterococcus faecalis ATCC 29212]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF
Download Length: 342 bp
>T10090 NZ_CP008816:c2000714-2000373 [Enterococcus faecalis ATCC 29212]
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGCCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCAAGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGCCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCAAGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG
Antitoxin
Download Length: 77 a.a. Molecular weight: 8677.67 Da Isoelectric Point: 4.3313
>AT10090 WP_002354773.1 NZ_CP008816:c2000944-2000714 [Enterococcus faecalis ATCC 29212]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF
Download Length: 231 bp
>AT10090 NZ_CP008816:c2000944-2000714 [Enterococcus faecalis ATCC 29212]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GCCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GCCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T6137 | Lactobacillus rhamnosus Lc 705 |
42.727 |
97.345 |
0.416 |
| T6142 | Lactobacillus rhamnosus ATCC 8530 |
42.727 |
97.345 |
0.416 |
| T6238 | Lactobacillus rhamnosus ATCC 8530 |
42.727 |
97.345 |
0.416 |
| T10069 | Klebsiella pneumoniae subsp. pneumoniae MGH 78578 |
34.906 |
94.643 |
0.33 |
| T137 | Deinococcus radiodurans R1 |
36.364 |
87.611 |
0.319 |
| T6120 | Xylella fastidiosa |
33.333 |
91.667 |
0.306 |
| T10067 | Agrobacterium tumefaciens str. C58 |
30.631 |
98.23 |
0.301 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT1076 | Shigella flexneri 2b |
30.952 |
100 |
0.342 |
Multiple sequence alignment
References
(1) Zhen Li et al. (2020) Characteristic and role of chromosomal type II toxin-antitoxin systems locus in Enterococcus faecalis ATCC29212. Journal of Microbiology (Seoul, Korea) 58(12):1027-1036. [PubMed:33095389]