Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) pemIK/-
Location 91354..91945 Replicon plasmid R100
Accession NC_002134
Organism Shigella flexneri 2b

Toxin (Protein)


Gene name pemK Uniprot ID Q7AK50
Locus tag HXF40_RS00560 Protein ID WP_001398199.1
Coordinates 91544..91945 (+) Length 134 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID V0TH33
Locus tag HXF40_RS00555 Protein ID WP_000557619.1
Coordinates 91354..91611 (+) Length 86 a.a.

Genomic Context

HXF40_RS00520HXF40_RS00525HXF40_RS00530HXF40_RS00535HXF40_RS00540HXF40_RS00545HXF40_RS00550HXF40_RS00555HXF40_RS00560HXF40_RS00565HXF40_RS00570
Location: 86669..87130 (462 bp)
Type: Others
Protein ID: WP_001233850.1
Location: 87176..87385 (210 bp)
Type: Others
Protein ID: WP_001298565.1
Location: 87423..88013 (591 bp)
Type: Others
Protein ID: WP_000766807.1
Location: 88253..88507 (255 bp)
Type: Others
Protein ID: WP_000083830.1
Location: 88745..88819 (75 bp)
Type: Others
Protein ID: WP_001365705.1
Location: 88812..89669 (858 bp)
Type: Others
Protein ID: WP_000130646.1
Location: 90608..91261 (654 bp)
Type: Others
Protein ID: WP_000616807.1
Location: 91354..91611 (258 bp)
Type: Antitoxin
Protein ID: WP_000557619.1
Location: 91544..91945 (402 bp)
Type: Toxin
Protein ID: WP_001398199.1
Location: 92230..93540 (1311 bp)
Type: Others
Protein ID: WP_001262765.1
Location: 93569..94266 (698 bp)
Type: Others
Protein ID: WP_095033700.1
ToxinAntitoxinOther genes
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Locus tag
Coordinates
Strand
Size (bp)
Protein ID
Product
Description
HXF40_RS00520 (D742_p2007)86669..87130+462WP_001233850.1thermonuclease family protein-
HXF40_RS0052587176..87385+210WP_001298565.1hemolysin expression modulator Hha-
HXF40_RS00530 (D742_p2006)87423..88013+591WP_000766807.1DUF2726 domain-containing protein-
HXF40_RS00535 (D742_p2096)88253..88507+255WP_000083830.1replication regulatory protein RepA-
HXF40_RS00540 (D742_p2094)88745..88819+75WP_001365705.1RepA leader peptide Tap-
HXF40_RS00545 (D742_p2097)88812..89669+858WP_000130646.1incFII family plasmid replication initiator RepA-
HXF40_RS00550 (D742_p2085)90608..91261+654WP_000616807.1CPBP family intramembrane glutamic endopeptidase-
HXF40_RS00555 (D742_p2102)91354..91611+258WP_000557619.1type II toxin-antitoxin system antitoxin PemIAntitoxin
HXF40_RS00560 (D742_p2101)91544..91945+402WP_001398199.1type II toxin-antitoxin system toxin endoribonuclease PemKToxin
HXF40_RS00565 (D742_p2005)92230..93540+1311WP_001262765.1DUF262 domain-containing protein-
HXF40_RS00570 (D742_p2113)93569..94266+698WP_095033700.1IS1-like element IS1B family transposase-

Associated MGEs

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MGE
detail
Similar
MGEs
Relative
position
MGE Type
Cargo ARG
Virulence gene
Coordinates
Length (bp)
-insideConjugative plasmidsul1 / qacE / ant(3'')-Ia / catA1 / tet(B)-1..9428194281


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(24-132)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14822.25 Da        Isoelectric Point: 11.5854

>T1076 WP_001398199.1 NC_002134:91544-91945 [Shigella flexneri 2b]
MLKYQLKNENGWMHRRLVRRKSDMERGEIWLVSLDPTAGHEQQGTRPVLIVTPAAFNRVTRLPVVVPVTSGGNFARTAGF
AVSLDGVGIRTTGVVRCDQPRTIDMKARGGKRLERVPETIMNEVLGRLSTILT

Download         Length: 402 bp

>T1076 NC_002134:91544-91945 [Shigella flexneri 2b]
ATGCTGAAATATCAGCTGAAGAACGAGAATGGCTGGATGCACCGGCGACTGGTCAGGAGGAAATCTGACATGGAAAGAGG
GGAAATCTGGCTTGTCTCGCTTGATCCTACCGCAGGTCATGAGCAGCAGGGAACGCGGCCGGTGCTGATTGTCACACCGG
CGGCCTTTAATCGCGTGACCCGCCTGCCTGTTGTTGTGCCCGTAACCAGCGGAGGCAATTTTGCCCGCACTGCCGGCTTT
GCGGTGTCGTTGGATGGTGTTGGCATACGTACCACAGGTGTTGTACGTTGCGATCAACCCCGGACAATTGATATGAAAGC
ACGGGGCGGAAAACGACTCGAACGGGTTCCGGAGACTATCATGAACGAAGTTCTTGGCCGCCTGTCCACTATTCTGACTT
GA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9332.61 Da        Isoelectric Point: 4.2694

>AT1076 WP_000557619.1 NC_002134:91354-91611 [Shigella flexneri 2b]
MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIVEPYRRPQYSLAELLAQCDPNAEISAEEREWLDAPAT
GQEEI

Download         Length: 258 bp

>AT1076 NC_002134:91354-91611 [Shigella flexneri 2b]
ATGCATACCACCCGACTGAAGAGGGTTGGCGGCTCAGTTATGCTGACCGTCCCACCGGCACTGCTGAATGCGCTGTCTCT
GGGCACAGATAATGAAGTTGGCATGGTCATTGATAATGGCCGGCTGATTGTTGAGCCGTACAGACGCCCGCAATATTCAC
TGGCTGAGCTACTGGCACAGTGTGATCCGAATGCTGAAATATCAGCTGAAGAACGAGAATGGCTGGATGCACCGGCGACT
GGTCAGGAGGAAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

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Protein
Organism
Identities (%)
Coverage (%)
Ha-value
T6120Xylella fastidiosa

50.459

100

0.509

T1049Escherichia coli str. K-12 substr. MG1655

43.86

98.276

0.431

T10069Klebsiella pneumoniae subsp. pneumoniae MGH 78578

37.383

95.536

0.357

T10067Agrobacterium tumefaciens str. C58

37.5

94.118

0.353

T137Deinococcus radiodurans R1

39.423

88.889

0.35

T10131Legionella pneumophila subsp. pneumophila str. Lorraine

35.238

99.057

0.349

T663Streptococcus mutans UA159

32.11

99.091

0.318
Showing 1 to 7 of 7 rows

Multiple sequence alignment    


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Protein
Organism
Identities (%)
Coverage (%)
Ha-value
AT6120Xylella fastidiosa

50.575

100

0.518

AT10067Agrobacterium tumefaciens str. C58

38.824

100

0.388

AT1049Escherichia coli str. K-12 substr. MG1655

37.179

93.976

0.349

AT10090Enterococcus faecalis ATCC 29212

30.952

100

0.342

AT10101Nitrospira sp. ND1 isolate Nitrospira sp. strain ND1

32.11

100

0.321

AT1044Escherichia coli str. K-12 substr. MG1655

39.062

78.049

0.305

AT10130Escherichia coli str. K-12 substr. MC4100 strain K-12

39.062

78.049

0.305
Showing 1 to 7 of 7 rows

Multiple sequence alignment    




Structures

Toxin

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Source
ID
Structure
PDB2C06
PDB1M1F

Antitoxin

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Source
ID
Structure
AlphaFold DBV0TH33

References

(1) Junjie Zhang et al. (2004) Interference of mRNA function by sequence-specific endoribonuclease PemK. The Journal of Biological Chemistry 279(20):20678-84. [PubMed:15024022] experimental


experimental experimental literature