Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) pemIK/-
Location 91354..91945 Replicon plasmid R100
Accession NC_002134
Organism Shigella flexneri 2b

Toxin (Protein)


Gene name pemK Uniprot ID Q7AK50
Locus tag HXF40_RS00560 Protein ID WP_001398199.1
Coordinates 91544..91945 (+) Length 134 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID V0TH33
Locus tag HXF40_RS00555 Protein ID WP_000557619.1
Coordinates 91354..91611 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXF40_RS00520 (D742_p2007) 86669..87130 + 462 WP_001233850.1 thermonuclease family protein -
HXF40_RS00525 87176..87385 + 210 WP_001298565.1 hemolysin expression modulator Hha -
HXF40_RS00530 (D742_p2006) 87423..88013 + 591 WP_000766807.1 DUF2726 domain-containing protein -
HXF40_RS00535 (D742_p2096) 88253..88507 + 255 WP_000083830.1 replication regulatory protein RepA -
HXF40_RS00540 (D742_p2094) 88745..88819 + 75 WP_001365705.1 RepA leader peptide Tap -
HXF40_RS00545 (D742_p2097) 88812..89669 + 858 WP_000130646.1 incFII family plasmid replication initiator RepA -
HXF40_RS00550 (D742_p2085) 90608..91261 + 654 WP_000616807.1 CPBP family intramembrane glutamic endopeptidase -
HXF40_RS00555 (D742_p2102) 91354..91611 + 258 WP_000557619.1 type II toxin-antitoxin system antitoxin PemI Antitoxin
HXF40_RS00560 (D742_p2101) 91544..91945 + 402 WP_001398199.1 type II toxin-antitoxin system toxin endoribonuclease PemK Toxin
HXF40_RS00565 (D742_p2005) 92230..93540 + 1311 WP_001262765.1 DUF262 domain-containing protein -
HXF40_RS00570 (D742_p2113) 93569..94266 + 698 WP_095033700.1 IS1-like element IS1B family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul1 / qacE / ant(3'')-Ia / catA1 / tet(B) - 1..94281 94281


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(24-132)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14822.25 Da        Isoelectric Point: 11.5854

>T1076 WP_001398199.1 NC_002134:91544-91945 [Shigella flexneri 2b]
MLKYQLKNENGWMHRRLVRRKSDMERGEIWLVSLDPTAGHEQQGTRPVLIVTPAAFNRVTRLPVVVPVTSGGNFARTAGF
AVSLDGVGIRTTGVVRCDQPRTIDMKARGGKRLERVPETIMNEVLGRLSTILT

Download         Length: 402 bp

>T1076 NC_002134:91544-91945 [Shigella flexneri 2b]
ATGCTGAAATATCAGCTGAAGAACGAGAATGGCTGGATGCACCGGCGACTGGTCAGGAGGAAATCTGACATGGAAAGAGG
GGAAATCTGGCTTGTCTCGCTTGATCCTACCGCAGGTCATGAGCAGCAGGGAACGCGGCCGGTGCTGATTGTCACACCGG
CGGCCTTTAATCGCGTGACCCGCCTGCCTGTTGTTGTGCCCGTAACCAGCGGAGGCAATTTTGCCCGCACTGCCGGCTTT
GCGGTGTCGTTGGATGGTGTTGGCATACGTACCACAGGTGTTGTACGTTGCGATCAACCCCGGACAATTGATATGAAAGC
ACGGGGCGGAAAACGACTCGAACGGGTTCCGGAGACTATCATGAACGAAGTTCTTGGCCGCCTGTCCACTATTCTGACTT
GA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9332.61 Da        Isoelectric Point: 4.2694

>AT1076 WP_000557619.1 NC_002134:91354-91611 [Shigella flexneri 2b]
MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIVEPYRRPQYSLAELLAQCDPNAEISAEEREWLDAPAT
GQEEI

Download         Length: 258 bp

>AT1076 NC_002134:91354-91611 [Shigella flexneri 2b]
ATGCATACCACCCGACTGAAGAGGGTTGGCGGCTCAGTTATGCTGACCGTCCCACCGGCACTGCTGAATGCGCTGTCTCT
GGGCACAGATAATGAAGTTGGCATGGTCATTGATAATGGCCGGCTGATTGTTGAGCCGTACAGACGCCCGCAATATTCAC
TGGCTGAGCTACTGGCACAGTGTGATCCGAATGCTGAAATATCAGCTGAAGAACGAGAATGGCTGGATGCACCGGCGACT
GGTCAGGAGGAAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T6120 Xylella fastidiosa

50.459

100

0.509
T1049 Escherichia coli str. K-12 substr. MG1655

43.86

98.276

0.431
T10069 Klebsiella pneumoniae subsp. pneumoniae MGH 78578

37.383

95.536

0.357
T10067 Agrobacterium tumefaciens str. C58

37.5

94.118

0.353
T137 Deinococcus radiodurans R1

39.423

88.889

0.35
T10131 Legionella pneumophila subsp. pneumophila str. Lorraine

35.238

99.057

0.349
T663 Streptococcus mutans UA159

32.11

99.091

0.318

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT6120 Xylella fastidiosa

50.575

100

0.518
AT10067 Agrobacterium tumefaciens str. C58

38.824

100

0.388
AT1049 Escherichia coli str. K-12 substr. MG1655

37.179

93.976

0.349
AT10090 Enterococcus faecalis ATCC 29212

30.952

100

0.342
AT10101 Nitrospira sp. ND1 isolate Nitrospira sp. strain ND1

32.11

100

0.321
AT1044 Escherichia coli str. K-12 substr. MG1655

39.062

78.049

0.305
AT10130 Escherichia coli str. K-12 substr. MC4100 strain K-12

39.062

78.049

0.305

Multiple sequence alignment    




Structures

Toxin

Source ID Structure
PDB 2C06
PDB 1M1F

Antitoxin

Source ID Structure
AlphaFold DB V0TH33

References

(1) Junjie Zhang et al. (2004) Interference of mRNA function by sequence-specific endoribonuclease PemK. The Journal of Biological Chemistry 279(20):20678-84. [PubMed:15024022] experimental


experimental experimental literature