10131

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) mazEF/-
Location 102897..103437 Replicon plasmid pLELO
Accession NC_018141
Organism Legionella pneumophila subsp. pneumophila str. Lorraine

Toxin (Protein)


Gene name mazF Uniprot ID A0A7G4RL43
Locus tag LPO_RS16205 Protein ID WP_014845117.1
Coordinates 102897..103217 (-) Length 107 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A2H5FRI5
Locus tag LPO_RS16210 Protein ID WP_014845118.1
Coordinates 103204..103437 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPO_RS16180 (LPO_p0106) 97909..98211 - 303 WP_014845112.1 transposase -
LPO_RS16185 (LPO_p0107) 98837..99328 - 492 WP_014845113.1 Hsp20/alpha crystallin family protein -
LPO_RS16190 (LPO_p0108) 99476..100063 - 588 WP_014845114.1 Hsp20/alpha crystallin family protein -
LPO_RS17355 100428..100583 - 156 WP_154080667.1 hypothetical protein -
LPO_RS16545 100655..101146 + 492 WP_051009158.1 IS91 family transposase -
LPO_RS16550 (LPO_p0111) 101168..101701 + 534 WP_061637559.1 IS91 family transposase -
LPO_RS16200 (LPO_p0113) 102067..102459 + 393 Protein_108 methyltransferase domain-containing protein -
LPO_RS16205 (LPO_p0114) 102897..103217 - 321 WP_014845117.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LPO_RS16210 (LPO_p0115) 103204..103437 - 234 WP_014845118.1 hypothetical protein Antitoxin
LPO_RS16215 (LPO_p0117) 103546..103893 - 348 WP_080031944.1 WGR domain-containing protein -
LPO_RS16220 (LPO_p0118) 103999..104883 - 885 WP_014845120.1 recombinase family protein -
LPO_RS16225 (LPO_p0119) 105096..105356 + 261 WP_014845121.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
LPO_RS16230 (LPO_p0120) 105349..105630 + 282 WP_014845122.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family -
LPO_RS16235 (LPO_p0121) 105810..108308 - 2499 WP_014845123.1 DEAD/DEAH box helicase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Non-Mobilizable plasmid blaOXA-29 - 1..150432 150432


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 107 a.a.        Molecular weight: 11444.32 Da        Isoelectric Point: 9.4321

>T10131 WP_014845117.1 NC_018141:c103217-102897 [Legionella pneumophila subsp. pneumophila]
MNRGDVYWVNLDPTTGSEINKLRPCVLVGATPINQARRTVVVVPLSSSATARPPITITVSCLGKQVTAVCDQIRTIDKSR
LKKVAGNLSDKDLNALDDGLRQVLCL

Download         Length: 321 bp

>T10131 NC_018141:c103217-102897 [Legionella pneumophila subsp. pneumophila]
ATGAATCGTGGTGATGTATATTGGGTAAACCTCGACCCTACTACAGGCTCTGAAATCAACAAACTCAGACCTTGCGTTTT
AGTTGGAGCAACACCGATTAATCAAGCAAGACGCACTGTTGTCGTCGTTCCTCTTTCAAGCTCTGCTACAGCCAGACCAC
CAATTACCATCACTGTTTCTTGTCTTGGCAAACAAGTTACCGCAGTTTGTGATCAAATAAGAACGATTGATAAAAGTCGT
TTAAAAAAAGTGGCAGGAAACCTTTCAGATAAAGATTTAAATGCCTTGGATGATGGGTTACGACAAGTTTTATGCCTTTG
A

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8932.14 Da        Isoelectric Point: 4.8047

>AT10131 WP_014845118.1 NC_018141:c103437-103204 [Legionella pneumophila subsp. pneumophila]
MNKVLISIPDQIASRMRAAIPQRQRSKVIAHLIEKEIERREKALYECALAVENDHGLQNEMNDWDITVQDGLTDESW

Download         Length: 234 bp

>AT10131 NC_018141:c103437-103204 [Legionella pneumophila subsp. pneumophila]
ATGAATAAAGTTTTAATATCAATACCCGATCAAATCGCTTCTAGAATGAGAGCAGCAATACCTCAGCGACAACGCAGCAA
GGTGATTGCTCATCTTATTGAAAAAGAAATTGAACGACGTGAAAAAGCGCTCTATGAATGCGCTTTAGCTGTTGAAAATG
ATCATGGTTTACAAAATGAAATGAACGATTGGGATATAACGGTTCAAGATGGATTAACTGATGAATCGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
AT10101 Nitrospira sp. ND1 isolate Nitrospira sp. strain ND1

41.758

85.849

0.358

Multiple sequence alignment    




Structures

Toxin

Source ID Structure
AlphaFold DB A0A7G4RL43

Antitoxin

Source ID Structure
AlphaFold DB A0A2H5FRI5

References

(1) Mao Shaku et al. (2018) Identification of MazF Homologue in Legionella pneumophila Which Cleaves RNA at the AACU Sequence. Journal of Molecular Microbiology And Biotechnology 28(6):269-280. [PubMed:30893701] experimental


experimental experimental literature