10101

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) mazEF/-
Location 530539..531110 Replicon chromosome
Accession NZ_FWEX01000005
Organism Nitrospira sp. ND1 isolate Nitrospira sp. strain ND1

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag NSND_RS02605 Protein ID WP_080877477.1
Coordinates 530539..530868 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag NSND_RS02610 Protein ID WP_080877478.1
Coordinates 530856..531110 (-) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NSND_RS02590 (NSND_50517) 528476..528835 + 360 WP_080877474.1 type II toxin-antitoxin system RelE/ParE family toxin -
NSND_RS02595 (NSND_50518) 528832..529224 + 393 WP_080877475.1 helix-turn-helix transcriptional regulator -
NSND_RS02600 (NSND_50519) 529727..530206 + 480 WP_080877476.1 NUDIX hydrolase -
NSND_RS02605 (NSND_50520) 530539..530868 - 330 WP_080877477.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
NSND_RS02610 (NSND_50521) 530856..531110 - 255 WP_080877478.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
NSND_RS21795 (NSND_50522) 531374..531499 + 126 WP_255373830.1 hypothetical protein -
NSND_RS20835 (NSND_50523) 531640..532368 + 729 WP_159450595.1 UPF0149 family protein -
NSND_RS02620 (NSND_50526) 532634..532945 - 312 WP_200810462.1 type II toxin-antitoxin system PemK/MazF family toxin -
NSND_RS02625 532971..533207 - 237 WP_080877479.1 hypothetical protein -
NSND_RS02630 (NSND_50527) 533329..534573 - 1245 WP_143833360.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

mRNA interferase PemK-like

(7-107)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 9783.00 Da        Isoelectric Point: 6.7543

>T10101 WP_080877478.1 NZ_FWEX01000005:c530868-530539 [Nitrospira sp. ND1]
MDMVVSRFDVFLVRLDATQGREIRKTRPCLIISPDEMNQHIDTVIIAPMTTKGRPYPTRVPLRFKGKSGQVVLDQIRTVD
KSRLVKRVGTITDVTRAQILTLLAELFAP

Download         Length: 330 bp

>T10101 NZ_FWEX01000005:c530868-530539 [Nitrospira sp. ND1]
ATGGACATGGTAGTGTCCCGCTTCGATGTCTTTCTCGTCCGTCTCGATGCTACACAAGGACGTGAGATCCGTAAGACCCG
CCCCTGTCTCATCATTTCCCCGGATGAAATGAATCAGCACATCGACACGGTCATCATCGCTCCCATGACGACGAAAGGCC
GTCCCTACCCGACTCGTGTCCCGCTTCGATTCAAAGGTAAGTCCGGGCAAGTTGTGCTGGATCAAATCCGGACAGTGGAC
AAAAGCCGATTGGTCAAGCGTGTAGGAACGATCACTGACGTGACACGCGCCCAGATCCTCACTCTGCTTGCCGAACTCTT
TGCCCCGTAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 12361.61 Da        Isoelectric Point: 11.0158

>AT10101 WP_080877477.1 NZ_FWEX01000005:c531110-530856 [Nitrospira sp. ND1]
MKARVVRIGNSQGIRIPKSVIEQCHLHGDVDLEIQHGQLVIRAASKPRAGWRQAFEQMHRHEDDHLLDEESAAMSTWDRS
EWTW

Download         Length: 255 bp

>AT10101 NZ_FWEX01000005:c531110-530856 [Nitrospira sp. ND1]
ATGAAAGCTCGTGTTGTGCGAATCGGCAATTCCCAGGGCATCCGGATTCCGAAATCTGTCATTGAACAGTGTCACCTGCA
CGGCGACGTCGATCTGGAGATTCAGCACGGACAATTGGTTATTCGCGCGGCATCCAAACCGCGCGCCGGCTGGCGTCAAG
CGTTTGAGCAGATGCATCGACACGAGGATGACCACCTGCTGGATGAAGAATCTGCAGCGATGTCTACATGGGATCGGAGC
GAATGGACATGGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References

(1) Rie Aoi et al. (2020) MazF Endoribonucleolytic Toxin Conserved in Nitrospira Specifically Cleaves the AACU, AACG, and AAUU Motifs. Toxins 12(5):287. [PubMed:32365819] experimental


experimental experimental literature