10094

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) mazEF/-
Location 101823..102352 Replicon chromosome
Accession NZ_JPPU01000003
Organism Staphylococcus aureus strain HG003 isolate RN1 Scaffold_3

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag IM26_RS18050 Protein ID WP_000621175.1
Coordinates 101990..102352 (+) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID T1YCG8
Locus tag IM26_RS18045 Protein ID WP_000948331.1
Coordinates 101823..101993 (+) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IM26_RS18015 (IM26_02890) 96859..97419 + 561 WP_001092411.1 K(+)-transporting ATPase subunit C -
IM26_RS18020 (IM26_02895) 97628..98107 + 480 WP_001287088.1 hypothetical protein -
IM26_RS18025 (IM26_02900) 98100..99683 + 1584 WP_001294626.1 PH domain-containing protein -
IM26_RS18030 (IM26_02905) 99670..100161 + 492 WP_001205910.1 PH domain-containing protein -
IM26_RS18035 (IM26_02910) 100165..100524 + 360 WP_000581200.1 holo-ACP synthase -
IM26_RS18040 (IM26_02915) 100590..101738 + 1149 WP_001281145.1 alanine racemase -
IM26_RS18045 (IM26_02920) 101823..101993 + 171 WP_000948331.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
IM26_RS18050 (IM26_02925) 101990..102352 + 363 WP_000621175.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
IM26_RS18055 (IM26_02930) 102701..103702 + 1002 WP_000390829.1 PP2C family protein-serine/threonine phosphatase -
IM26_RS18060 (IM26_02935) 103821..104147 + 327 WP_001052491.1 anti-sigma factor antagonist -
IM26_RS18065 (IM26_02940) 104149..104628 + 480 WP_001190829.1 anti-sigma B factor RsbW -
IM26_RS18070 (IM26_02945) 104603..105373 + 771 WP_001041103.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13441.69 Da        Isoelectric Point: 10.1654

>T10094 WP_000621175.1 NZ_JPPU01000003:101990-102352 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Download         Length: 363 bp

>T10094 NZ_JPPU01000003:101990-102352 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6251.78 Da        Isoelectric Point: 3.8305

>AT10094 WP_000948331.1 NZ_JPPU01000003:101823-101993 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Download         Length: 171 bp

>AT10094 NZ_JPPU01000003:101823-101993 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.


Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0VRZ1

References

(1) Roberto Sierra et al. (2020) Insights into the global effect on Staphylococcus aureus growth arrest by induction of the endoribonuclease MazF toxin. Nucleic Acids Research 48(15):8545-8561. [PubMed:32735661] experimental


experimental experimental literature