655

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) chpIK/RHH-MazF
Location 2824394..2824970 Replicon chromosome
Accession NC_004342
Organism Leptospira interrogans serovar Lai str. 56601

Toxin (Protein)


Gene name chpK Uniprot ID Q93MT8
Locus tag LA_RS11750 Protein ID WP_000617906.1
Coordinates 2824228..2824569 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name chpI Uniprot ID Q93MT9
Locus tag LA_RS11755 Protein ID WP_000844758.1
Coordinates 2824556..2824804 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LA_RS11730 (LA2835) 2819749..2820153 - 405 WP_001974506.1 pyridoxamine 5'-phosphate oxidase family protein -
LA_RS11735 (LA2836) 2820150..2820617 - 468 WP_000060051.1 hypothetical protein -
LA_RS11740 (LA2840) 2821421..2822356 + 936 WP_000004530.1 ornithine carbamoyltransferase -
LA_RS11745 (LA2841) 2822496..2823488 + 993 WP_000041092.1 aldolase -
LA_RS11750 (LA2843) 2824228..2824569 - 342 WP_000617906.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LA_RS11755 (LA2844) 2824556..2824804 - 249 WP_000844758.1 ribbon-helix-helix domain-containing protein Antitoxin
LA_RS11760 (LA2845) 2825621..2825917 - 297 WP_000477061.1 helix-turn-helix domain-containing protein -
LA_RS11765 2826071..2826530 + 460 Protein_2328 hypothetical protein -
LA_RS11770 (LA2847) 2827172..2829154 - 1983 WP_000836895.1 HD family phosphohydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(2-41)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12372.25 Da        Isoelectric Point: 6.3272

>T655 WP_000617906.1 NC_004342:c2824569-2824228 [Leptospira interrogans serovar Lai str. 56601]
MIRGEIWWVDLGIPFGSEPGFKRPVLIIQDDSFNQSNINTIVSIAITSNLNLSEAPGNVFISKKDSSLSKDSVINVSQIV
TLDKERFLNKAGKLKSNKLGEVEIGLKLVTGLD

Download         Length: 342 bp

>T655 NC_004342:c2824569-2824228 [Leptospira interrogans serovar Lai str. 56601]
ATGATTCGTGGTGAAATTTGGTGGGTAGACTTGGGAATTCCATTTGGAAGTGAGCCTGGATTTAAACGTCCAGTTCTTAT
AATTCAAGATGACTCTTTTAATCAAAGTAATATTAATACAATAGTTTCGATTGCAATTACATCGAATTTAAATCTATCTG
AAGCACCAGGTAATGTTTTCATTAGTAAAAAAGATTCGAGTTTATCAAAGGACTCGGTCATAAATGTTTCTCAAATTGTG
ACTTTAGATAAAGAAAGATTTCTTAACAAAGCGGGTAAACTTAAATCAAATAAATTAGGTGAAGTTGAAATTGGGCTTAA
GTTGGTTACAGGCTTAGATTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9380.77 Da        Isoelectric Point: 10.3815

>AT655 WP_000844758.1 NC_004342:c2824804-2824556 [Leptospira interrogans serovar Lai str. 56601]
MKTAISIPDELFRAAEKIAKKLGIPRSQLFAKALEEFIQSHSKESVTEKLNKIYSNKSKETRNNITDLSVESLRKSLKND
SW

Download         Length: 249 bp

>AT655 NC_004342:c2824804-2824556 [Leptospira interrogans serovar Lai str. 56601]
ATGAAGACGGCGATTTCAATTCCTGATGAGTTATTTAGGGCTGCCGAGAAAATAGCTAAAAAACTTGGGATTCCTCGAAG
CCAGTTATTTGCAAAAGCATTAGAAGAATTTATCCAATCTCATAGTAAAGAATCTGTAACTGAGAAATTAAATAAAATCT
ACAGTAATAAATCCAAAGAAACTAGAAATAATATTACTGACTTATCCGTAGAATCGCTTCGTAAGAGTTTAAAAAATGAT
TCGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MS74

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HD92

References

(1) M Picardeau et al. (2001) Killing effect and antitoxic activity of the Leptospira interrogans toxin-antitoxin system in Escherichia coli. Journal of Bacteriology 183(21):6494-7. [PubMed:11591696] experimental


experimental experimental literature