Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) mazEF/-
Location 3935641..3936280 Replicon chromosome
Accession NC_013316
Organism Clostridium difficile R20291

Toxin (Protein)


Gene name mazF Uniprot ID D5PZG4
Locus tag CDR20291_RS17750 Protein ID WP_003421356.1
Coordinates 3935641..3936024 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID Q180W1
Locus tag CDR20291_RS17755 Protein ID WP_003421359.1
Coordinates 3935993..3936280 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDR20291_RS17725 (CDR20291_3292) 3930799..3931368 + 570 WP_009892015.1 5-formyltetrahydrofolate cyclo-ligase -
CDR20291_RS17730 (CDR20291_3293) 3931550..3931966 - 417 WP_009894025.1 hypothetical protein -
CDR20291_RS17735 (CDR20291_3294) 3932192..3933286 - 1095 WP_003437819.1 hypothetical protein -
CDR20291_RS17740 (CDR20291_3295) 3933709..3934650 - 942 WP_009892019.1 transketolase family protein -
CDR20291_RS17745 (CDR20291_3296) 3934643..3935470 - 828 WP_003421354.1 transketolase -
CDR20291_RS17750 (CDR20291_3297) 3935641..3936024 - 384 WP_003421356.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CDR20291_RS17755 (CDR20291_3298) 3935993..3936280 - 288 WP_003421359.1 ribbon-helix-helix protein, CopG family Antitoxin
CDR20291_RS17760 (CDR20291_3299) 3936313..3937470 - 1158 WP_003437830.1 alanine racemase -
CDR20291_RS17765 (CDR20291_3300) 3937485..3938075 - 591 WP_009892025.1 hypothetical protein -
CDR20291_RS17770 (CDR20291_3301) 3938096..3938557 - 462 WP_003421362.1 CBS domain-containing protein -
CDR20291_RS17775 (CDR20291_3302) 3938572..3938952 - 381 WP_003437831.1 holo-ACP synthase -
CDR20291_RS17780 (CDR20291_3303) 3939414..3939674 - 261 WP_003425849.1 ATP synthase F1 subunit epsilon -
CDR20291_RS17785 (CDR20291_3304) 3939677..3941071 - 1395 WP_003425850.1 F0F1 ATP synthase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(13-124)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14092.36 Da        Isoelectric Point: 8.4694

>T10096 WP_003421356.1 NC_013316:c3936024-3935641 [Clostridioides difficile R20291]
MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQNDVGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGL
NKDSVILLEQIRTIDKKRLREKIGCLDENMMIKVDDGLQISLGLFTF

Download         Length: 384 bp

>T10096 NC_013316:c3936024-3935641 [Clostridioides difficile R20291]
ATGAAGGGAAGATTGCCCAAGGTGAGTACTAATTGTGAAATAAAGCGTGGTGATTTATATTACGCTGATTTAAGTCCAGT
TGTTGGTTCAGAGCAGGGAGGCGTTAGACCTGTTCTGATAATACAAAATGATGTAGGTAATAAATATAGTCCTACAGTTA
TAGTATCAGCTATAACATCTCAGATTAATAAAGCCAAATTACCTACTCATGTAGAGATAAGTTCCAATGAATATGGGCTT
AATAAAGACTCAGTAATTCTTTTAGAGCAAATTAGAACTATCGATAAAAAGAGACTGAGGGAAAAAATTGGTTGTCTAGA
TGAAAATATGATGATAAAAGTAGATGATGGACTTCAAATAAGTTTAGGATTGTTTACTTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10825.20 Da        Isoelectric Point: 4.5942

>AT10096 WP_003421359.1 NC_013316:c3936280-3935993 [Clostridioides difficile R20291]
VYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYITQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEV
SSDDKNEGKIAQGEY

Download         Length: 288 bp

>AT10096 NC_013316:c3936280-3935993 [Clostridioides difficile R20291]
GTGTACGATAAGAGTAAAGAAAAAATAGAAGTTACTTTACCTGACTCTCTGATTTCTGAAGTTGATAGTATAGTTCAGAT
GGAGAACAGTAACAGAGAAGATTTTGCAAAAGCTGCTTTTCAGTTCTATATAACTCAGAAAAAGAAAATTCATATTAAAG
AATCTATGAAAACGGGTTATAGAGAAATGGGTCAAATTAATCTGTCACTAGCTGAATTAGGTATGACAGTTGAAGAAGTA
TCTTCTGATGATAAAAATGAAGGGAAGATTGCCCAAGGTGAGTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A069AP01

Antitoxin

Source ID Structure
AlphaFold DB A0A125YDX2

References

(1) R Álvarez et al. (2020) Evaluation of functionality of type II toxin-antitoxin systems of Clostridioides difficile R20291. Microbiological Research 239:126539. [PubMed:32622285] experimental


experimental experimental literature