Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/- |
| Location | 3935641..3936280 | Replicon | chromosome |
| Accession | NC_013316 | ||
| Organism | Clostridium difficile R20291 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | D5PZG4 |
| Locus tag | CDR20291_RS17750 | Protein ID | WP_003421356.1 |
| Coordinates | 3935641..3936024 (-) | Length | 128 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | Q180W1 |
| Locus tag | CDR20291_RS17755 | Protein ID | WP_003421359.1 |
| Coordinates | 3935993..3936280 (-) | Length | 96 a.a. |
Genomic Context
Location: 3930799..3931368 (570 bp)
Type: Others
Protein ID: WP_009892015.1
Type: Others
Protein ID: WP_009892015.1
Location: 3931550..3931966 (417 bp)
Type: Others
Protein ID: WP_009894025.1
Type: Others
Protein ID: WP_009894025.1
Location: 3932192..3933286 (1095 bp)
Type: Others
Protein ID: WP_003437819.1
Type: Others
Protein ID: WP_003437819.1
Location: 3933709..3934650 (942 bp)
Type: Others
Protein ID: WP_009892019.1
Type: Others
Protein ID: WP_009892019.1
Location: 3934643..3935470 (828 bp)
Type: Others
Protein ID: WP_003421354.1
Type: Others
Protein ID: WP_003421354.1
Location: 3935641..3936024 (384 bp)
Type: Toxin
Protein ID: WP_003421356.1
Type: Toxin
Protein ID: WP_003421356.1
Location: 3935993..3936280 (288 bp)
Type: Antitoxin
Protein ID: WP_003421359.1
Type: Antitoxin
Protein ID: WP_003421359.1
Location: 3936313..3937470 (1158 bp)
Type: Others
Protein ID: WP_003437830.1
Type: Others
Protein ID: WP_003437830.1
Location: 3937485..3938075 (591 bp)
Type: Others
Protein ID: WP_009892025.1
Type: Others
Protein ID: WP_009892025.1
Location: 3938096..3938557 (462 bp)
Type: Others
Protein ID: WP_003421362.1
Type: Others
Protein ID: WP_003421362.1
Location: 3938572..3938952 (381 bp)
Type: Others
Protein ID: WP_003437831.1
Type: Others
Protein ID: WP_003437831.1
Location: 3939414..3939674 (261 bp)
Type: Others
Protein ID: WP_003425849.1
Type: Others
Protein ID: WP_003425849.1
Location: 3939677..3941071 (1395 bp)
Type: Others
Protein ID: WP_003425850.1
Type: Others
Protein ID: WP_003425850.1
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Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CDR20291_RS17725 (CDR20291_3292) | 3930799..3931368 | + | 570 | WP_009892015.1 | 5-formyltetrahydrofolate cyclo-ligase | - |
| CDR20291_RS17730 (CDR20291_3293) | 3931550..3931966 | - | 417 | WP_009894025.1 | hypothetical protein | - |
| CDR20291_RS17735 (CDR20291_3294) | 3932192..3933286 | - | 1095 | WP_003437819.1 | hypothetical protein | - |
| CDR20291_RS17740 (CDR20291_3295) | 3933709..3934650 | - | 942 | WP_009892019.1 | transketolase family protein | - |
| CDR20291_RS17745 (CDR20291_3296) | 3934643..3935470 | - | 828 | WP_003421354.1 | transketolase | - |
| CDR20291_RS17750 (CDR20291_3297) | 3935641..3936024 | - | 384 | WP_003421356.1 | type II toxin-antitoxin system PemK/MazF family toxin | Toxin |
| CDR20291_RS17755 (CDR20291_3298) | 3935993..3936280 | - | 288 | WP_003421359.1 | ribbon-helix-helix protein, CopG family | Antitoxin |
| CDR20291_RS17760 (CDR20291_3299) | 3936313..3937470 | - | 1158 | WP_003437830.1 | alanine racemase | - |
| CDR20291_RS17765 (CDR20291_3300) | 3937485..3938075 | - | 591 | WP_009892025.1 | hypothetical protein | - |
| CDR20291_RS17770 (CDR20291_3301) | 3938096..3938557 | - | 462 | WP_003421362.1 | CBS domain-containing protein | - |
| CDR20291_RS17775 (CDR20291_3302) | 3938572..3938952 | - | 381 | WP_003437831.1 | holo-ACP synthase | - |
| CDR20291_RS17780 (CDR20291_3303) | 3939414..3939674 | - | 261 | WP_003425849.1 | ATP synthase F1 subunit epsilon | - |
| CDR20291_RS17785 (CDR20291_3304) | 3939677..3941071 | - | 1395 | WP_003425850.1 | F0F1 ATP synthase subunit beta | - |
Associated MGEs
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MGE detail | Similar MGEs | Relative position | MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| No matching records found | |||||||
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 128 a.a. Molecular weight: 14092.36 Da Isoelectric Point: 8.4694
>T10096 WP_003421356.1 NC_013316:c3936024-3935641 [Clostridioides difficile R20291]
MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQNDVGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGL
NKDSVILLEQIRTIDKKRLREKIGCLDENMMIKVDDGLQISLGLFTF
MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQNDVGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGL
NKDSVILLEQIRTIDKKRLREKIGCLDENMMIKVDDGLQISLGLFTF
Download Length: 384 bp
>T10096 NC_013316:c3936024-3935641 [Clostridioides difficile R20291]
ATGAAGGGAAGATTGCCCAAGGTGAGTACTAATTGTGAAATAAAGCGTGGTGATTTATATTACGCTGATTTAAGTCCAGT
TGTTGGTTCAGAGCAGGGAGGCGTTAGACCTGTTCTGATAATACAAAATGATGTAGGTAATAAATATAGTCCTACAGTTA
TAGTATCAGCTATAACATCTCAGATTAATAAAGCCAAATTACCTACTCATGTAGAGATAAGTTCCAATGAATATGGGCTT
AATAAAGACTCAGTAATTCTTTTAGAGCAAATTAGAACTATCGATAAAAAGAGACTGAGGGAAAAAATTGGTTGTCTAGA
TGAAAATATGATGATAAAAGTAGATGATGGACTTCAAATAAGTTTAGGATTGTTTACTTTTTAA
ATGAAGGGAAGATTGCCCAAGGTGAGTACTAATTGTGAAATAAAGCGTGGTGATTTATATTACGCTGATTTAAGTCCAGT
TGTTGGTTCAGAGCAGGGAGGCGTTAGACCTGTTCTGATAATACAAAATGATGTAGGTAATAAATATAGTCCTACAGTTA
TAGTATCAGCTATAACATCTCAGATTAATAAAGCCAAATTACCTACTCATGTAGAGATAAGTTCCAATGAATATGGGCTT
AATAAAGACTCAGTAATTCTTTTAGAGCAAATTAGAACTATCGATAAAAAGAGACTGAGGGAAAAAATTGGTTGTCTAGA
TGAAAATATGATGATAAAAGTAGATGATGGACTTCAAATAAGTTTAGGATTGTTTACTTTTTAA
Antitoxin
Download Length: 96 a.a. Molecular weight: 10825.20 Da Isoelectric Point: 4.5942
>AT10096 WP_003421359.1 NC_013316:c3936280-3935993 [Clostridioides difficile R20291]
VYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYITQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEV
SSDDKNEGKIAQGEY
VYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYITQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEV
SSDDKNEGKIAQGEY
Download Length: 288 bp
>AT10096 NC_013316:c3936280-3935993 [Clostridioides difficile R20291]
GTGTACGATAAGAGTAAAGAAAAAATAGAAGTTACTTTACCTGACTCTCTGATTTCTGAAGTTGATAGTATAGTTCAGAT
GGAGAACAGTAACAGAGAAGATTTTGCAAAAGCTGCTTTTCAGTTCTATATAACTCAGAAAAAGAAAATTCATATTAAAG
AATCTATGAAAACGGGTTATAGAGAAATGGGTCAAATTAATCTGTCACTAGCTGAATTAGGTATGACAGTTGAAGAAGTA
TCTTCTGATGATAAAAATGAAGGGAAGATTGCCCAAGGTGAGTACTAA
GTGTACGATAAGAGTAAAGAAAAAATAGAAGTTACTTTACCTGACTCTCTGATTTCTGAAGTTGATAGTATAGTTCAGAT
GGAGAACAGTAACAGAGAAGATTTTGCAAAAGCTGCTTTTCAGTTCTATATAACTCAGAAAAAGAAAATTCATATTAAAG
AATCTATGAAAACGGGTTATAGAGAAATGGGTCAAATTAATCTGTCACTAGCTGAATTAGGTATGACAGTTGAAGAAGTA
TCTTCTGATGATAAAAATGAAGGGAAGATTGCCCAAGGTGAGTACTAA
Similar Proteins
Only experimentally validated proteins are listed.
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Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T1499 | Bacillus anthracis str. Sterne | 75.439 | 98.276 | 0.741 |
| T134 | Bacillus subtilis subsp. subtilis str. 168 | 72.807 | 98.276 | 0.716 |
| T10177 | Staphylococcus epidermidis isolate BPH0662 | 73.684 | 95 | 0.7 |
| T10078 | Staphylococcus aureus strain HG003 isolate RN1 Scaffold_3 | 72.973 | 92.5 | 0.675 |
| T612 | Staphylococcus aureus subsp. aureus MW2 | 72.973 | 92.5 | 0.675 |
| T10094 | Staphylococcus aureus strain HG003 isolate RN1 Scaffold_3 | 72.973 | 92.5 | 0.675 |
| T1073 | Staphylococcus aureus subsp. aureus N315 | 72.973 | 92.5 | 0.675 |
| T6090 | Staphylococcus equorum | 68.75 | 93.333 | 0.642 |
| T6135 | Lactobacillus rhamnosus GG | 60.684 | 92.126 | 0.559 |
| T6141 | Lactobacillus rhamnosus ATCC 8530 | 60.684 | 92.126 | 0.559 |
Multiple sequence alignment
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Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| No matching records found | ||||
Structures
Toxin
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Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A069AP01 |
Antitoxin
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Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A125YDX2 |
References
(1) R Álvarez et al. (2020) Evaluation of functionality of type II toxin-antitoxin systems of Clostridioides difficile R20291. Microbiological Research 239:126539. [PubMed:32622285]