Detailed information of TA system TA010177

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/-
• Location: 1934159..1934688
• Replicon: chromosome
• Accession: NZ_LT571449
• Organism: Staphylococcus epidermidis isolate BPH0662

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q5HME7
• Locus tag: BN6763_RS09900
• Protein ID: WP_001829891.1
• Coordinates: 1934159..1934521 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: Q5HME6
• Locus tag: BN6763_RS09905
• Protein ID: WP_001829931.1
• Coordinates: 1934518..1934688 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BN6763_RS09880	1931160..1931930	-	771	WP_002468952.1	RNA polymerase sigma factor SigB	-
BN6763_RS09885	1931905..1932384	-	480	WP_001829903.1	anti-sigma B factor RsbW	-
BN6763_RS09890	1932386..1932712	-	327	WP_001829952.1	anti-sigma factor antagonist	-
BN6763_RS09895	1932812..1933813	-	1002	WP_002504876.1	PP2C family protein-serine/threonine phosphatase	-
BN6763_RS09900	1934159..1934521	-	363	WP_001829891.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BN6763_RS09905	1934518..1934688	-	171	WP_001829931.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
BN6763_RS09910	1934775..1935923	-	1149	WP_002468951.1	alanine racemase	-
BN6763_RS09915	1935989..1936342	-	354	WP_001829915.1	holo-ACP synthase	-
BN6763_RS09920	1936390..1936899	-	510	WP_001829888.1	PH domain-containing protein	-
BN6763_RS09925	1936886..1938391	-	1506	WP_001829976.1	PH domain-containing protein	-
BN6763_RS09930	1938384..1938863	-	480	WP_001829909.1	PH domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13497.65 Da
• Isoelectric Point: 9.9522

>T10177 WP_001829891.1 NZ_LT571449:c1934521-1934159 [Staphylococcus epidermidis]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTFLSESKMIEVDNALDISLGLNNFDHHKS

Nucleotide

• Length: 363 bp

>T10177 NZ_LT571449:c1934521-1934159 [Staphylococcus epidermidis]
ATGATTAGAAGAGGAGATGTTTATTTAGCGGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAGTAAGACCTGTAGT
TATCATTCAAAATGATACTGGTAATAAATATAGTCCAACTGTAATTGTAGCTGCGATTACTGGTAGGATTAATAAAGCGA
AAATACCAACCCACGTAGAAATTGAAAAGAAAAAGTATAAATTAGACAAAGATTCAGTTATTCTTCTTGAACAAATTAGA
ACACTAGATAAAAAGCGTTTAAAAGAAAAATTAACATTTTTATCAGAGAGTAAAATGATAGAGGTTGATAATGCCTTAGA
TATTAGTTTGGGATTAAATAACTTTGATCATCATAAATCTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6289.92 Da
• Isoelectric Point: 4.1920

>AT10177 WP_001829931.1 NZ_LT571449:c1934688-1934518 [Staphylococcus epidermidis]
MLSFNQNRNHSLEQSLKEGYAQMADLNLSLATEAFPIECEACDCNESHLISNSKNE

Nucleotide

• Length: 171 bp

>AT10177 NZ_LT571449:c1934688-1934518 [Staphylococcus epidermidis]
ATGTTATCTTTTAATCAAAATAGAAACCACAGTCTTGAACAATCTTTAAAAGAAGGTTATGCACAAATGGCCGATTTAAA
CCTCTCCCTAGCAACAGAAGCTTTTCCGATAGAGTGTGAAGCTTGTGATTGCAATGAATCACATTTAATATCTAATTCAA
AGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T10094	Staphylococcus aureus strain HG003 isolate RN1 Scaffold_3	91.667	100	0.917
T612	Staphylococcus aureus subsp. aureus MW2	91.667	100	0.917
T10078	Staphylococcus aureus strain HG003 isolate RN1 Scaffold_3	91.667	100	0.917
T1073	Staphylococcus aureus subsp. aureus N315	91.667	100	0.917
T6090	Staphylococcus equorum	88.333	100	0.883
T10096	Clostridium difficile R20291	73.684	95	0.7
T1499	Bacillus anthracis str. Sterne	70.435	99.138	0.698
T134	Bacillus subtilis subsp. subtilis str. 168	66.087	99.138	0.655
T6141	Lactobacillus rhamnosus ATCC 8530	60.504	99.167	0.6
T6135	Lactobacillus rhamnosus GG	60.504	99.167	0.6
T6136	Lactobacillus rhamnosus Lc 705	60.504	99.167	0.6
T6140	Lactobacillus rhamnosus GG	60.504	99.167	0.6
T10042	Candidatus Desulforudis audaxviator MP104C	46.847	94.068	0.441
T655	Leptospira interrogans serovar Lai str. 56601	43.243	98.23	0.425
T6247	Mycobacterium tuberculosis H37Rv	37.383	93.86	0.351
T10131	Legionella pneumophila subsp. pneumophila str. Lorraine	32.381	99.057	0.321
T911	Nitrosomonas europaea ATCC 19718	40	79.646	0.319

Multiple sequence alignment

Antitoxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
AT612	Staphylococcus aureus subsp. aureus MW2	85.714	100	0.857
AT1073	Staphylococcus aureus subsp. aureus N315	85.714	100	0.857
AT10078	Staphylococcus aureus strain HG003 isolate RN1 Scaffold_3	85.714	100	0.857
AT10094	Staphylococcus aureus strain HG003 isolate RN1 Scaffold_3	85.714	100	0.857
AT6090	Staphylococcus equorum	76.786	100	0.768
AT1499	Bacillus anthracis str. Sterne	32.075	94.643	0.304

Multiple sequence alignment

Structures

Toxin

• AlphaFold DB: A0A2G7HWR0

Antitoxin

• AlphaFold DB: A0A0N1EF65

References

(1) Sadeghi Kalani Behrooz et al. (2018) Study of MazEF, sam, and phd-doc putative toxin-antitoxin systems in Staphylococcus epidermidis. Acta Microbiologica Et Immunologica Hungarica 65(1):81-91. [PubMed:29471693]


 in silico analysis literature