6140

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) pemIK/RHH-MazF
Location 2596564..2597287 Replicon chromosome
Accession NC_017482
Organism Lactobacillus rhamnosus GG

Toxin (Protein)


Gene name pemK Uniprot ID K8Q4Y0
Locus tag LRHM_RS12110 Protein ID WP_005686631.1
Coordinates 2596564..2596947 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K8QH19
Locus tag LRHM_RS12115 Protein ID WP_005686632.1
Coordinates 2596967..2597215 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRHM_RS12105 (LRHM_2430) 2595600..2596127 - 528 WP_005686630.1 QueT transporter family protein -
LRHM_RS15305 2596165..2596362 - 198 WP_064536013.1 hypothetical protein -
LRHM_RS12110 (LRHM_2431) 2596564..2596947 - 384 WP_005686631.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LRHM_RS12115 (LRHM_2432) 2596967..2597215 - 249 WP_005686632.1 antitoxin Antitoxin
LRHM_RS12120 (LRHM_2433) 2597327..2598466 - 1140 WP_005686633.1 alanine racemase -
LRHM_RS12125 (LRHM_2434) 2598453..2598827 - 375 WP_005686634.1 holo-ACP synthase -
LRHM_RS12130 (LRHM_2435) 2598996..2600504 - 1509 WP_005686635.1 DEAD/DEAH box helicase -
LRHM_RS12135 (LRHM_2436) 2600778..2602166 - 1389 WP_015765066.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-115)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13757.03 Da        Isoelectric Point: 10.9764

>T6140 WP_005686631.1 NC_017482:c2596947-2596564 [Lactobacillus rhamnosus GG]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T6140 NC_017482:c2596947-2596564 [Lactobacillus rhamnosus GG]
ATGGATGTAACAGTCAAACGCGGGGATGTATTTTTCGCCGATTTATCGCCGGTTGTCGGGTCGGAACAAGGCGGCAACCG
GCCCGTACTTATTATTCAAAATAACGTTGGCAATCACTATAGTCCCACCGTCATTGTGGCTGCCATTACCTCCAAAATCC
AAAAACCCAAAATGCCCACTCATGTCGGTTTGCGCGCCAAGCAAGACGGGGTTGAGCGTAATTCAGTCATTTTGCTTGAA
CAGATTCGTACAATTGATAAGCAACGGTTACAGGCACGCGTTACCGCTCTTTCAAGTGCCAAAATGGCAGCGGTGGATCG
AGCACTGGCGATATCGGTCGGACTCGTCAGCTTGCCTAAGCCGAAAACATATAATAAAAACTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8936.13 Da        Isoelectric Point: 4.4778

>AT6140 WP_005686632.1 NC_017482:c2597215-2596967 [Lactobacillus rhamnosus GG]
MKKETSVTVALDSAIKARLDAYCELNDMGVQDVITEAVAKFLQSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT6140 NC_017482:c2597215-2596967 [Lactobacillus rhamnosus GG]
ATGAAGAAGGAAACGTCAGTAACGGTCGCGCTTGATAGTGCTATCAAAGCGCGTTTGGATGCCTATTGTGAGTTGAATGA
TATGGGTGTTCAGGATGTCATTACTGAAGCAGTGGCGAAGTTTTTGCAGTCACAAGCACCTGAGATCAATCAGTTGATTT
CGGGCTATGTGGCAATGGGACAAATCAATACGGAAATCAGCCATGCTTTCTCCGCCTGCGAAAGTGAGGCATACGCCCAT
ATTCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
AT6135 Lactobacillus rhamnosus GG

100

100

1
AT6136 Lactobacillus rhamnosus Lc 705

100

100

1
AT6141 Lactobacillus rhamnosus ATCC 8530

100

100

1
AT10101 Nitrospira sp. ND1 isolate Nitrospira sp. strain ND1

39.091

100

0.394

Multiple sequence alignment    




Structures

Toxin

Source ID Structure
AlphaFold DB K8Q4Y0

Antitoxin

Source ID Structure
AlphaFold DB K8QH19

References

(1) K M Klimina et al. (2013) Identification and characterization of toxin-antitoxin systems in strains of Lactobacillus rhamnosus isolated from humans. Anaerobe 22:82-9. [PubMed:23727113] experimental


experimental experimental literature