6136

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) pemIK/RHH-MazF
Location 2581133..2581784 Replicon chromosome
Accession NC_013199
Organism Lactobacillus rhamnosus Lc 705

Toxin (Protein)


Gene name pemK Uniprot ID K8Q4Y0
Locus tag LC705_RS12025 Protein ID WP_005686631.1
Coordinates 2581133..2581516 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K8QH19
Locus tag LC705_RS12030 Protein ID WP_005686632.1
Coordinates 2581536..2581784 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LC705_RS12015 (LC705_02536) 2580092..2580619 - 528 WP_005691221.1 QueT transporter family protein -
LC705_RS12025 (LC705_02539) 2581133..2581516 - 384 WP_005686631.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LC705_RS12030 (LC705_02540) 2581536..2581784 - 249 WP_005686632.1 antitoxin Antitoxin
LC705_RS12035 (LC705_02541) 2581896..2583035 - 1140 WP_005691215.1 alanine racemase -
LC705_RS12040 (LC705_02542) 2583022..2583396 - 375 WP_005686634.1 holo-ACP synthase -
LC705_RS12045 (LC705_02543) 2583565..2585073 - 1509 WP_005686635.1 DEAD/DEAH box helicase -
LC705_RS12050 (LC705_02544) 2585347..2586735 - 1389 WP_014571607.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-115)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13757.03 Da        Isoelectric Point: 10.9764

>T6136 WP_005686631.1 NC_013199:c2581516-2581133 [Lactobacillus rhamnosus Lc 705]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T6136 NC_013199:c2581516-2581133 [Lactobacillus rhamnosus Lc 705]
ATGGATGTAACAGTCAAACGCGGGGATGTATTTTTCGCCGATTTATCGCCGGTTGTCGGGTCGGAACAAGGCGGCAACCG
GCCCGTACTTATTATTCAAAACAATGTTGGTAACCATTATAGCCCCACTGTTATCGTGGCTGCCATTACCTCCAAAATCC
AAAAACCCAAAATGCCCACCCATGTCGGTTTGCGCGCCAAGCAAGACGGGGTTGAGCGTAATTCAGTCATTTTGCTTGAA
CAGATTCGTACAATTGATAAGCAACGGTTACAGGCACGCGTTACCGCTCTTTCAAGTGCCAAAATGGCAGCGGTGGATCG
GGCACTGGCGATATCGGTCGGACTCGTCAGCTTGCCTAAGCCGAAAACATATAATAAAAACTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8936.13 Da        Isoelectric Point: 4.4778

>AT6136 WP_005686632.1 NC_013199:c2581784-2581536 [Lactobacillus rhamnosus Lc 705]
MKKETSVTVALDSAIKARLDAYCELNDMGVQDVITEAVAKFLQSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT6136 NC_013199:c2581784-2581536 [Lactobacillus rhamnosus Lc 705]
ATGAAGAAGGAAACGTCAGTAACGGTCGCGCTTGATAGTGCTATCAAAGCGCGTTTGGATGCCTATTGCGAGTTGAATGA
TATGGGTGTTCAGGATGTCATTACTGAAGCAGTGGCGAAGTTTTTGCAGTCACAAGCACCTGAGATCAATCAATTGATTT
CTGGCTATGTGGCAATGGGACAAATCAATACGGAAATCAGCCATGCTTTCTCCGCCTGCGAAAGTGAGGCATACGCCCAT
ATTCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
AT6135 Lactobacillus rhamnosus GG

100

100

1
AT6141 Lactobacillus rhamnosus ATCC 8530

100

100

1
AT6140 Lactobacillus rhamnosus GG

100

100

1
AT10101 Nitrospira sp. ND1 isolate Nitrospira sp. strain ND1

39.091

100

0.394

Multiple sequence alignment    




Structures

Toxin

Source ID Structure
AlphaFold DB K8Q4Y0

Antitoxin

Source ID Structure
AlphaFold DB K8QH19

References

(1) K M Klimina et al. (2013) Identification and characterization of toxin-antitoxin systems in strains of Lactobacillus rhamnosus isolated from humans. Anaerobe 22:82-9. [PubMed:23727113] experimental


experimental experimental literature