612

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) mazEF/RHH-MazF
Location 2149382..2149911 Replicon chromosome
Accession NC_003923
Organism Staphylococcus aureus subsp. aureus MW2

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag MW1992 Protein ID 21283721
Coordinates 2149382..2149744 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag MW1993 Protein ID 21283722
Coordinates 2149741..2149911 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW_RS10840 (MW1988) 2146362..2147132 - 771 WP_001041108.1 RNA polymerase sigma factor SigB -
MW_RS10845 (MW1989) 2147107..2147586 - 480 WP_001190830.1 anti-sigma B factor RsbW -
MW_RS10850 (MW1990) 2147588..2147914 - 327 WP_001052491.1 anti-sigma factor antagonist -
MW_RS10855 (MW1991) 2148033..2149034 - 1002 WP_000390829.1 PP2C family protein-serine/threonine phosphatase -
MW_RS10865 (MW1992) 2149382..2149744 - 363 WP_000621175.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MW_RS10870 (MW1993) 2149741..2149911 - 171 WP_000948331.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
MW_RS10875 (MW1994) 2149996..2151144 - 1149 WP_001281154.1 alanine racemase -
MW_RS10880 (MW1995) 2151210..2151569 - 360 WP_000581197.1 holo-ACP synthase -
MW_RS10885 (MW1996) 2151573..2152064 - 492 WP_001205912.1 PH domain-containing protein -
MW_RS10890 (MW1997) 2152051..2153634 - 1584 WP_001294626.1 PH domain-containing protein -
MW_RS10895 (MW1998) 2153627..2154106 - 480 WP_001287078.1 hypothetical protein -
MW_RS10900 (MW1999) 2154315..2154875 - 561 WP_001092409.1 K(+)-transporting ATPase subunit C -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13441.69 Da        Isoelectric Point: 10.1654

>T612 21283721 NC_003923:c2149744-2149382 [Staphylococcus aureus subsp. aureus MW2]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Download         Length: 363 bp

>T612 NC_003923:c2149744-2149382 [Staphylococcus aureus subsp. aureus MW2]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6251.78 Da        Isoelectric Point: 3.8305

>AT612 21283722 NC_003923:c2149911-2149741 [Staphylococcus aureus subsp. aureus MW2]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Download         Length: 171 bp

>AT612 NC_003923:c2149911-2149741 [Staphylococcus aureus subsp. aureus MW2]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.


Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References

(1) Gul Habib et al. (2018) Bioinformatics and Functional Assessment of Toxin-Antitoxin Systems in Staphylococcus aureus. Toxins 10(11):473. [PubMed:30441856] experimental


experimental experimental literature