Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) mazEF/-
Location 1279373..1279949 Replicon chromosome
Accession NC_004757
Organism Nitrosomonas europaea ATCC 19718

Toxin (Protein)


Gene name mazF Uniprot ID Q82VB5
Locus tag ALW85_RS06130 Protein ID WP_011111770.1
Coordinates 1279373..1279714 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag ALW85_RS06135 Protein ID WP_041356688.1
Coordinates 1279707..1279937 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ALW85_RS06100 (NE1175) 1275245..1275739 + 495 WP_011111766.1 disulfide bond formation protein B -
ALW85_RS06105 (NE1176) 1275767..1276555 + 789 WP_011111767.1 peptidoglycan-binding protein -
ALW85_RS06110 (NE1177) 1276713..1277102 + 390 WP_041356684.1 RidA family protein -
ALW85_RS06115 (NE1178) 1277247..1278290 - 1044 WP_011110809.1 IS481 family transposase -
ALW85_RS06120 (NE1179) 1278486..1278995 - 510 WP_011111768.1 hypothetical protein -
ALW85_RS06130 (NE1181) 1279373..1279714 - 342 WP_011111770.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
ALW85_RS06135 (NE1182) 1279707..1279937 - 231 WP_041356688.1 hypothetical protein Antitoxin
ALW85_RS06140 (NE1183) 1280204..1282648 - 2445 WP_011111772.1 S8 family peptidase -
ALW85_RS06145 (NE1184) 1282842..1283570 - 729 WP_011111773.1 1-acyl-sn-glycerol-3-phosphate acyltransferase -
ALW85_RS06150 (NE1185) 1283570..1284112 - 543 WP_011111774.1 D-glycero-beta-D-manno-heptose 1,7-bisphosphate 7-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1277247..1278290 1043


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13145.42 Da        Isoelectric Point: 10.3155

>T911 WP_011111770.1 NC_004757:c1279714-1279373 [Nitrosomonas europaea ATCC 19718]
MTDFKQRDIYWIDLEPTKGAETRKLRPCVIIQSDLVNVQSRTVIVAPLLLQHKPWPFAVNLEPTEKNGLDKDRHINLKQL
RAVDISRIGKKQGRLENRYKDPIKAALMIIFDL

Download         Length: 342 bp

>T911 NC_004757:c1279714-1279373 [Nitrosomonas europaea ATCC 19718]
ATGACTGATTTCAAGCAGCGGGATATTTACTGGATCGATCTTGAACCGACAAAGGGTGCGGAAACAAGAAAATTAAGGCC
ATGTGTAATTATTCAAAGTGACCTGGTTAACGTTCAATCCAGAACAGTGATAGTTGCCCCTTTGCTCCTTCAGCATAAAC
CCTGGCCATTTGCAGTGAATCTGGAGCCCACAGAAAAAAATGGTCTGGATAAGGATCGTCATATCAACCTCAAGCAATTA
CGCGCGGTTGATATTTCACGCATTGGAAAAAAACAAGGCAGGCTTGAAAATAGATACAAGGATCCTATCAAAGCAGCTTT
AATGATCATCTTTGATTTGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8649.48 Da        Isoelectric Point: 4.5026

>AT911 WP_041356688.1 NC_004757:c1279937-1279707 [Nitrosomonas europaea ATCC 19718]
MTHRVTITLDAETFAFLNDVASSNRSAYVNQLLKQDRKNFLQAALRKANQEEAEDTNYQEKLQAWESTLSDGLAND

Download         Length: 231 bp

>AT911 NC_004757:c1279937-1279707 [Nitrosomonas europaea ATCC 19718]
ATGACTCATAGAGTGACTATTACGCTCGACGCAGAAACATTTGCTTTTCTGAACGATGTTGCAAGTAGTAATCGTAGCGC
CTATGTTAATCAGCTCTTAAAACAGGATCGCAAAAATTTCCTGCAGGCAGCGCTACGCAAAGCGAATCAGGAAGAGGCAG
AAGATACAAACTACCAGGAAAAATTACAGGCATGGGAAAGTACGCTGTCAGATGGATTAGCCAATGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
AT10101 Nitrospira sp. ND1 isolate Nitrospira sp. strain ND1

35.577

95.413

0.339

Multiple sequence alignment    




Structures

Toxin

Source ID Structure
AlphaFold DB Q82VB5

Antitoxin

Source ID Structure

References

(1) Tatsuki Miyamoto et al. (2016) AAU-Specific RNA Cleavage Mediated by MazF Toxin Endoribonuclease Conserved in Nitrosomonas europaea. Toxins 8(6):174. [PubMed:27271670] experimental


experimental experimental literature