6141

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) pemIK/RHH-MazF
Location 2500879..2501471 Replicon chromosome
Accession NC_017491
Organism Lactobacillus rhamnosus ATCC 8530

Toxin (Protein)


Gene name pemK Uniprot ID K8Q4Y0
Locus tag LRHK_RS11520 Protein ID WP_005686631.1
Coordinates 2501088..2501471 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K8QH19
Locus tag LRHK_RS11525 Protein ID WP_005686632.1
Coordinates 2501491..2501739 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRHK_RS11510 (LRHK_2557) 2500047..2500574 - 528 WP_005691221.1 QueT transporter family protein -
LRHK_RS11520 (LRHK_2558) 2501088..2501471 - 384 WP_005686631.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LRHK_RS11525 (LRHK_2560) 2501491..2501739 - 249 WP_005686632.1 antitoxin Antitoxin
LRHK_RS11530 (LRHK_2561) 2501851..2502990 - 1140 WP_005691215.1 alanine racemase -
LRHK_RS11535 (LRHK_2562) 2502977..2503351 - 375 WP_005686634.1 holo-ACP synthase -
LRHK_RS11540 (LRHK_2563) 2503520..2505028 - 1509 WP_005686635.1 DEAD/DEAH box helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 2494796..2598376 103580


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-115)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13757.03 Da        Isoelectric Point: 10.9764

>T6141 WP_005686631.1 NC_017491:c2501471-2501088 [Lactobacillus rhamnosus ATCC 8530]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T6141 NC_017491:c2501471-2501088 [Lactobacillus rhamnosus ATCC 8530]
ATGGATGTAACAGTCAAACGCGGGGATGTATTTTTCGCCGATTTATCGCCGGTTGTCGGGTCGGAACAAGGCGGCAACCG
GCCCGTACTTATTATTCAAAACAATGTTGGTAACCATTATAGCCCCACTGTTATCGTGGCTGCCATTACCTCCAAAATCC
AAAAACCCAAAATGCCCACCCATGTCGGTTTGCGCGCCAAGCAAGACGGGGTTGAGCGTAATTCAGTCATTTTGCTTGAA
CAGATTCGTACAATTGATAAGCAACGGTTACAGGCACGCGTTACCGCTCTTTCAAGTGCCAAAATGGCAGCGGTGGATCG
GGCACTGGCGATATCGGTCGGACTCGTCAGCTTGCCTAAGCCGAAAACATATAATAAAAACTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8936.13 Da        Isoelectric Point: 4.4778

>AT6141 WP_005686632.1 NC_017491:c2501739-2501491 [Lactobacillus rhamnosus ATCC 8530]
MKKETSVTVALDSAIKARLDAYCELNDMGVQDVITEAVAKFLQSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT6141 NC_017491:c2501739-2501491 [Lactobacillus rhamnosus ATCC 8530]
ATGAAGAAGGAAACGTCAGTAACGGTCGCGCTTGATAGTGCTATCAAAGCGCGTTTGGATGCCTATTGCGAGTTGAATGA
TATGGGTGTTCAGGATGTCATTACTGAAGCAGTGGCGAAGTTTTTGCAGTCACAAGCACCTGAGATCAATCAATTGATTT
CTGGCTATGTGGCAATGGGACAAATCAATACGGAAATCAGCCATGCTTTCTCCGCCTGCGAAAGTGAGGCATACGCCCAT
ATTCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
AT6135 Lactobacillus rhamnosus GG

100

100

1
AT6136 Lactobacillus rhamnosus Lc 705

100

100

1
AT6140 Lactobacillus rhamnosus GG

100

100

1
AT10101 Nitrospira sp. ND1 isolate Nitrospira sp. strain ND1

39.091

100

0.394

Multiple sequence alignment    




Structures

Toxin

Source ID Structure
AlphaFold DB K8Q4Y0

Antitoxin

Source ID Structure
AlphaFold DB K8QH19

References

(1) K M Klimina et al. (2013) Identification and characterization of toxin-antitoxin systems in strains of Lactobacillus rhamnosus isolated from humans. Anaerobe 22:82-9. [PubMed:23727113] experimental


experimental experimental literature