134

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) mazEF/RHH-MazF
Location 518218..518854 Replicon chromosome
Accession NC_000964
Organism Bacillus subtilis subsp. subtilis str. 168

Toxin (Protein)


Gene name mazF Uniprot ID P96622
Locus tag BSU_04660 Protein ID NP_388347.1
Coordinates 518943..519293 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P96621
Locus tag BSU_04650 Protein ID NP_388346.1
Coordinates 518657..518938 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BSU_04600 (BSU04600) 513283..514764 + 1482 NP_388341.1 resistance to heterologous antibiotics -
BSU_04610 (BSU04610) 515016..515615 - 600 NP_388342.1 putative rhomboid protease -
BSU_04620 (BSU04620) 515710..516075 + 366 NP_388343.1 holo-acyl carrier protein synthase (phosphopantetheinyl transferase) -
BSU_04630 (BSU04630) 516241..517257 + 1017 NP_388344.2 putative lipoprotein -
BSU_04640 (BSU04640) 517372..518541 + 1170 NP_388345.1 D-alanine racemase -
BSU_04650 (BSU04650) 518657..518938 + 282 NP_388346.1 antitoxin EndoAI Antitoxin
BSU_04660 (BSU04660) 518943..519293 + 351 NP_388347.1 endoribonuclease toxin Toxin
BSU_04670 (BSU04670) 519408..520232 + 825 NP_388348.1 component of the anxiosome (stressosome); positive regulation of sigma(B) activity in response to salt and heat stress -
BSU_04680 (BSU04680) 520237..520602 + 366 NP_388349.1 antagonist of RsbT -
BSU_04690 (BSU04690) 520606..521007 + 402 NP_388350.1 switch protein/serine-threonine kinase; controls the activity of the anxiosome (stressosome) -
BSU_04700 (BSU04700) 521019..522026 + 1008 NP_388351.1 protein serine phosphatase; controls the activity of the anxiosome (stressosome) -
BSU_04710 (BSU04710) 522088..522417 + 330 NP_388352.1 anti-anti-sigma factor (antagonist of RsbW) -
BSU_04720 (BSU04720) 522414..522896 + 483 NP_388353.1 switch protein/serine kinase and anti-sigma factor (inhibitory sigma-B binding protein) -
BSU_04730 (BSU04730) 522862..523650 + 789 NP_388354.2 RNA polymerase sigma-37 factor (sigma(B)) -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T134 NP_388347.1 NC_000964:518943-519293 [Bacillus subtilis subsp. subtilis str. 168]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T134 NC_000964:518943-519293 [Bacillus subtilis subsp. subtilis str. 168]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT134 NP_388346.1 NC_000964:518657-518938 [Bacillus subtilis subsp. subtilis str. 168]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT134 NC_000964:518657-518938 [Bacillus subtilis subsp. subtilis str. 168]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
AT1499 Bacillus anthracis str. Sterne

52.632

100

0.538

Multiple sequence alignment    




Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB P96621

References

(1) Olivier Pellegrini et al. (2005) The Bacillus subtilis ydcDE operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor. Molecular Microbiology 56(5):1139-48. [PubMed:15882409] experimental

(2) Dhirendra K Simanshu et al. (2013) Structural basis of mRNA recognition and cleavage by toxin MazF and its regulation by antitoxin MazE in Bacillus subtilis. Molecular Cell 52(3):447-58. [PubMed:24120662] experimental


experimental experimental literature