Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | pemIK/- |
| Location | 3467412..3467926 | Replicon | chromosome |
| Accession | NC_000962 | ||
| Organism | Mycobacterium tuberculosis H37Rv | ||
Toxin (Protein)
| Gene name | pemK | Uniprot ID | P96295 |
| Locus tag | Rv3098A | Protein ID | YP_007411812.1 |
| Coordinates | 3467606..3467926 (+) | Length | 107 a.a. |
Antitoxin (Protein)
| Gene name | pemI | Uniprot ID | - |
| Locus tag | - | Protein ID | - |
| Coordinates | 3467412..3467612 (+) | Length | 67 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Rv3094c | 3462761..3463891 | - | 1131 | NP_217610.1 | hypothetical protein | - |
| Rv3095 | 3463973..3464449 | + | 477 | NP_217611.1 | HTH-type transcriptional regulator | - |
| Rv3096 | 3464547..3465686 | + | 1140 | NP_217612.1 | hypothetical protein | - |
| Rv3097c | 3465778..3467091 | - | 1314 | YP_177924.1 | triacylglycerol lipase Lip | - |
| Rv3098c | 3467210..3467662 | - | 453 | NP_217614.1 | hypothetical protein | - |
| - | 3467412..3467612 | + | 201 | - | - | Antitoxin |
| Rv3098A | 3467606..3467926 | + | 321 | YP_007411812.1 | PemK-like protein | Toxin |
| Rv3099c | 3468413..3469264 | - | 852 | NP_217615.1 | hypothetical protein | - |
| Rv3100c | 3469301..3469783 | - | 483 | NP_217616.1 | SsrA-binding protein | - |
| Rv3101c | 3469786..3470679 | - | 894 | NP_217617.1 | cell division protein FtsX | - |
| Rv3102c | 3470680..3471369 | - | 690 | NP_217618.1 | cell division ATP-binding protein FtsE | - |
| Rv3103c | 3471413..3471850 | - | 438 | NP_217619.1 | hypothetical protein | - |
| Rv3104c | 3471852..3472778 | - | 927 | NP_217620.1 | transmembrane protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 107 a.a. Molecular weight: 11826.63 Da Isoelectric Point: 9.8132
>T10160 YP_007411812.1 NC_000962:3467606-3467926 [Mycobacterium tuberculosis H37Rv]
VVIRGAVYRVDFGDAKRGHEQRGRRYAVVISPGSMPWSVVTVVPTSTSAQPAVFRPELEVMGTKTRFLVDQIRTIGIVYV
HGDPVDYLDRDQMAKVEHAVARYLGL
VVIRGAVYRVDFGDAKRGHEQRGRRYAVVISPGSMPWSVVTVVPTSTSAQPAVFRPELEVMGTKTRFLVDQIRTIGIVYV
HGDPVDYLDRDQMAKVEHAVARYLGL
Download Length: 321 bp
>T10160 NC_000962:3467606-3467926 [Mycobacterium tuberculosis H37Rv]
GTGGTGATTCGGGGAGCGGTCTACAGGGTCGACTTCGGCGATGCGAAGCGAGGCCACGAGCAACGCGGGCGGCGCTACGC
CGTGGTCATCAGCCCCGGCTCGATGCCGTGGAGTGTAGTAACCGTGGTGCCGACGTCGACAAGCGCCCAACCTGCGGTTT
TCCGACCAGAGCTGGAAGTCATGGGAACAAAGACACGGTTCCTGGTGGATCAGATCCGGACGATCGGCATCGTCTATGTG
CACGGCGATCCGGTCGACTATCTGGACCGTGACCAAATGGCCAAGGTGGAACACGCCGTGGCACGATACCTTGGTCTGTG
A
GTGGTGATTCGGGGAGCGGTCTACAGGGTCGACTTCGGCGATGCGAAGCGAGGCCACGAGCAACGCGGGCGGCGCTACGC
CGTGGTCATCAGCCCCGGCTCGATGCCGTGGAGTGTAGTAACCGTGGTGCCGACGTCGACAAGCGCCCAACCTGCGGTTT
TCCGACCAGAGCTGGAAGTCATGGGAACAAAGACACGGTTCCTGGTGGATCAGATCCGGACGATCGGCATCGTCTATGTG
CACGGCGATCCGGTCGACTATCTGGACCGTGACCAAATGGCCAAGGTGGAACACGCCGTGGCACGATACCTTGGTCTGTG
A
Antitoxin
Download Length: 67 a.a. Molecular weight: 15695.89 Da Isoelectric Point: 11.7195
>AT10160 - NC_000962:3467412-3467612 [Mycobacterium tuberculosis H37Rv]
MTATIGFRPTEKDEQIINAAMRSGERKSDVIRRALQLLEREVWIKQARTDAERLRDEDVSTEPDAW
MTATIGFRPTEKDEQIINAAMRSGERKSDVIRRALQLLEREVWIKQARTDAERLRDEDVSTEPDAW
Download Length: 201 bp
>AT10160 NC_000962:3467412-3467612 [Mycobacterium tuberculosis H37Rv]
ATGACCGCGACCATCGGCTTCCGACCTACTGAAAAAGACGAGCAGATCATCAACGCCGCAATGCGCAGCGGCGAGCGCAA
GAGCGACGTCATCCGGCGGGCACTGCAGCTGCTCGAACGGGAAGTGTGGATCAAGCAAGCTCGCACCGACGCTGAGCGAC
TTCGAGACGAGGATGTCTCCACTGAACCGGACGCGTGGTGA
ATGACCGCGACCATCGGCTTCCGACCTACTGAAAAAGACGAGCAGATCATCAACGCCGCAATGCGCAGCGGCGAGCGCAA
GAGCGACGTCATCCGGCGGGCACTGCAGCTGCTCGAACGGGAAGTGTGGATCAAGCAAGCTCGCACCGACGCTGAGCGAC
TTCGAGACGAGGATGTCTCCACTGAACCGGACGCGTGGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T1499 | Bacillus anthracis str. Sterne |
31.579 |
100 |
0.34 |
| T134 | Bacillus subtilis subsp. subtilis str. 168 |
29.825 |
100 |
0.321 |
| T10042 | Candidatus Desulforudis audaxviator MP104C |
30.357 |
100 |
0.321 |
| T1073 | Staphylococcus aureus subsp. aureus N315 |
28.829 |
100 |
0.302 |
| T10094 | Staphylococcus aureus strain HG003 isolate RN1 Scaffold_3 |
28.829 |
100 |
0.302 |
| T10078 | Staphylococcus aureus strain HG003 isolate RN1 Scaffold_3 |
28.829 |
100 |
0.302 |
| T612 | Staphylococcus aureus subsp. aureus MW2 |
28.829 |
100 |
0.302 |
| T6120 | Xylella fastidiosa |
32.653 |
92.453 |
0.302 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
References
(1) Xiaodong Chi et al. (2018) Biochemical characterization of mt-PemIK, a novel toxin-antitoxin system in Mycobacterium tuberculosis. FEBS Letters 592(24):4039-4050. [PubMed:30372528]