Detailed information of TA system TA001499

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/RHH-MazF
• Location: 241201..241843
• Replicon: chromosome
• Accession: NC_005945
• Organism: Bacillus anthracis str. Sterne

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: J8CWW5
• Locus tag: BAS_RS01325
• Protein ID: WP_000635963.1
• Coordinates: 241493..241843 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: R8I8H2
• Locus tag: BAS_RS01320
• Protein ID: WP_000004570.1
• Coordinates: 241201..241488 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BAS_RS01295 (BAS0234)	236517..237479	+	963	WP_000961037.1	UV DNA damage repair endonuclease UvsE	-
BAS_RS01300 (BAS0235)	237472..238044	-	573	WP_000906916.1	rhomboid family intramembrane serine protease	-
BAS_RS01305 (BAS0236)	238137..238496	+	360	WP_000635040.1	holo-ACP synthase	-
BAS_RS01310 (BAS0237)	238653..239603	+	951	WP_025388382.1	outer membrane lipoprotein carrier protein LolA	-
BAS_RS01315 (BAS0238)	239722..240891	+	1170	WP_000390596.1	alanine racemase	-
BAS_RS01320 (BAS0239)	241201..241488	+	288	WP_000004570.1	antitoxin EndoAI	Antitoxin
BAS_RS01325 (BAS0240)	241493..241843	+	351	WP_000635963.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
BAS_RS01330 (BAS0241)	241911..244079	+	2169	WP_000426225.1	RNA-binding transcriptional accessory protein	-
BAS_RS01335	244137..244253	-	117	WP_001143642.1	cortex morphogenetic protein CmpA	-
BAS_RS01340 (BAS0242)	244449..244907	+	459	WP_000344248.1	SprT family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12974.12 Da
• Isoelectric Point: 5.7168

>T1499 WP_000635963.1 NC_005945:241493-241843 [Bacillus anthracis str. Sterne]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Nucleotide

• Length: 351 bp

>T1499 NC_005945:241493-241843 [Bacillus anthracis str. Sterne]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGTGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10780.36 Da
• Isoelectric Point: 8.9605

>AT1499 WP_000004570.1 NC_005945:241201-241488 [Bacillus anthracis str. Sterne]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Nucleotide

• Length: 288 bp

>AT1499 NC_005945:241201-241488 [Bacillus anthracis str. Sterne]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T134	Bacillus subtilis subsp. subtilis str. 168	93.966	100	0.94
T10096	Clostridium difficile R20291	75.439	98.276	0.741
T10177	Staphylococcus epidermidis isolate BPH0662	70.435	99.138	0.698
T6136	Lactobacillus rhamnosus Lc 705	69.298	98.276	0.681
T6141	Lactobacillus rhamnosus ATCC 8530	69.298	98.276	0.681
T6135	Lactobacillus rhamnosus GG	69.298	98.276	0.681
T6140	Lactobacillus rhamnosus GG	69.298	98.276	0.681
T10094	Staphylococcus aureus strain HG003 isolate RN1 Scaffold_3	69.643	96.552	0.672
T10078	Staphylococcus aureus strain HG003 isolate RN1 Scaffold_3	69.643	96.552	0.672
T1073	Staphylococcus aureus subsp. aureus N315	69.643	96.552	0.672
T612	Staphylococcus aureus subsp. aureus MW2	69.643	96.552	0.672
T6090	Staphylococcus equorum	66.964	96.552	0.647
T10042	Candidatus Desulforudis audaxviator MP104C	42.342	95.69	0.405
T6247	Mycobacterium tuberculosis H37Rv	38.261	100	0.386
T655	Leptospira interrogans serovar Lai str. 56601	37.273	97.345	0.363
T6252	Mycobacterium tuberculosis H37Rv	34.188	100	0.345
T10160	Mycobacterium tuberculosis H37Rv	31.579	100	0.34
T1049	Escherichia coli str. K-12 substr. MG1655	33.929	96.552	0.328
T911	Nitrosomonas europaea ATCC 19718	33.636	97.345	0.327
T10131	Legionella pneumophila subsp. pneumophila str. Lorraine	28.829	100	0.302

Multiple sequence alignment

Antitoxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
AT134	Bacillus subtilis subsp. subtilis str. 168	52.632	100	0.538
AT10177	Staphylococcus epidermidis isolate BPH0662	32.075	94.643	0.304
AT10101	Nitrospira sp. ND1 isolate Nitrospira sp. strain ND1	33.673	89.908	0.303

Multiple sequence alignment

Structures

Toxin

• PDB: 4HKE
• PDB: 7BXY

Antitoxin

• AlphaFold DB: A0A366FY90

References

(1) Shivangi Agarwal et al. (2007) Identification and characterization of a novel toxin-antitoxin module from Bacillus anthracis. FEBS Letters 581(9):1727-34. [PubMed:17416361]

(2) Shivangi Agarwal et al. (2010) PemK toxin of Bacillus anthracis is a ribonuclease: an insight into its active site, structure, and function. The Journal of Biological Chemistry 285(10):7254-70. [PubMed:20022964]


 experimental literature