Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/RHH-MazF |
| Location | 2234305..2234891 | Replicon | chromosome |
| Accession | NC_000962 | ||
| Organism | Mycobacterium tuberculosis H37Rv | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | P9WII3 |
| Locus tag | Rv1991c | Protein ID | NP_216507.1 |
| Coordinates | 2234305..2234649 (-) | Length | 115 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | P9WJ87 |
| Locus tag | Rv1991A | Protein ID | YP_007410673.1 |
| Coordinates | 2234643..2234891 (-) | Length | 83 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Rv1986 | 2230011..2230610 | + | 600 | NP_216502.1 | amino acid transporter | - |
| Rv1987 | 2231026..2231454 | + | 429 | NP_216503.1 | chitinase | - |
| Rv1988 | 2231680..2232219 | + | 540 | NP_216504.1 | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) | - |
| Rv1989c | 2232739..2233299 | - | 561 | NP_216505.1 | hypothetical protein | - |
| Rv1990c | 2233296..2233637 | - | 342 | NP_216506.1 | transcriptional regulator | - |
| Rv1991c | 2234305..2234649 | - | 345 | NP_216507.1 | mRNA interferase MazF6 | Toxin |
| Rv1991A | 2234643..2234891 | - | 249 | YP_007410673.1 | antitoxin MazE6 | Antitoxin |
| Rv1992c | 2234991..2237306 | - | 2316 | NP_216508.1 | cation transporter ATPase G | - |
| Rv1993c | 2237303..2237575 | - | 273 | NP_216509.1 | hypothetical protein | - |
| Rv1994c | 2237628..2237984 | - | 357 | NP_216510.1 | HTH-type transcriptional regulator CmtR | - |
| Rv1995 | 2238141..2238908 | + | 768 | NP_216511.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 115 a.a. Molecular weight: 12230.12 Da Isoelectric Point: 9.8887
>T6247 NP_216507.1 NC_000962:c2234649-2234305 [Mycobacterium tuberculosis H37Rv]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL
Download Length: 345 bp
>T6247 NC_000962:c2234649-2234305 [Mycobacterium tuberculosis H37Rv]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA
Antitoxin
Download Length: 83 a.a. Molecular weight: 9290.34 Da Isoelectric Point: 4.3757
>AT6247 YP_007410673.1 NC_000962:c2234891-2234643 [Mycobacterium tuberculosis H37Rv]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW
Download Length: 249 bp
>AT6247 NC_000962:c2234891-2234643 [Mycobacterium tuberculosis H37Rv]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA
Similar Proteins
Only experimentally validated proteins are listed.
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
References
(1) Hossein Kazemian et al. (2021) Comparison of toxin-antitoxin expression among drug-susceptible and drug-resistant clinical isolates of Mycobacterium tuberculosis. Advances in Respiratory Medicine 89(2):110-114. [PubMed:33966258]
(2) Amita Gupta (2009) Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. FEMS Microbiology Letters 290(1):45-53. [PubMed:19016878]
(3) Ji-Li Zhao et al. (2018) Viability, biofilm formation, and MazEF expression in drug-sensitive and drug-resistant Mycobacterium tuberculosis strains circulating in Xinjiang, China. Infection And Drug Resistance 11:345-358. [PubMed:29563815]
(4) Melissa V Ramirez et al. (2013) MazF6 toxin of Mycobacterium tuberculosis demonstrates antitoxin specificity and is coupled to regulation of cell growth by a Soj-like protein. BMC Microbiology 13:240. [PubMed:24172039]
(5) Holly R Ramage et al. (2009) Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genetics 5(12):e1000767. [PubMed:20011113]
(6) Longxuan Zhao et al. (2008) Biochemical characterization of a chromosomal toxin-antitoxin system in Mycobacterium tuberculosis. FEBS Letters 582(5):710-4. [PubMed:18258191]
(7) Soumyanetra Chandra et al. (2021) Rapid Identification of Secondary Structure and Binding Site Residues in an Intrinsically Disordered Protein Segment. Frontiers in Genetics 12:755292. [PubMed:34795695]