Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) mazEF/RHH-MazF
Location 2234305..2234891 Replicon chromosome
Accession NC_000962
Organism Mycobacterium tuberculosis H37Rv

Toxin (Protein)


Gene name mazF Uniprot ID P9WII3
Locus tag Rv1991c Protein ID NP_216507.1
Coordinates 2234305..2234649 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P9WJ87
Locus tag Rv1991A Protein ID YP_007410673.1
Coordinates 2234643..2234891 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Rv1986 2230011..2230610 + 600 NP_216502.1 amino acid transporter -
Rv1987 2231026..2231454 + 429 NP_216503.1 chitinase -
Rv1988 2231680..2232219 + 540 NP_216504.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
Rv1989c 2232739..2233299 - 561 NP_216505.1 hypothetical protein -
Rv1990c 2233296..2233637 - 342 NP_216506.1 transcriptional regulator -
Rv1991c 2234305..2234649 - 345 NP_216507.1 mRNA interferase MazF6 Toxin
Rv1991A 2234643..2234891 - 249 YP_007410673.1 antitoxin MazE6 Antitoxin
Rv1992c 2234991..2237306 - 2316 NP_216508.1 cation transporter ATPase G -
Rv1993c 2237303..2237575 - 273 NP_216509.1 hypothetical protein -
Rv1994c 2237628..2237984 - 357 NP_216510.1 HTH-type transcriptional regulator CmtR -
Rv1995 2238141..2238908 + 768 NP_216511.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T6247 NP_216507.1 NC_000962:c2234649-2234305 [Mycobacterium tuberculosis H37Rv]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T6247 NC_000962:c2234649-2234305 [Mycobacterium tuberculosis H37Rv]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT6247 YP_007410673.1 NC_000962:c2234891-2234643 [Mycobacterium tuberculosis H37Rv]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT6247 NC_000962:c2234891-2234643 [Mycobacterium tuberculosis H37Rv]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK0
PDB 5HK3
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB P9WJ87

References

(1) Hossein Kazemian et al. (2021) Comparison of toxin-antitoxin expression among drug-susceptible and drug-resistant clinical isolates of Mycobacterium tuberculosis. Advances in Respiratory Medicine 89(2):110-114. [PubMed:33966258] experimental

(2) Amita Gupta (2009) Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. FEMS Microbiology Letters 290(1):45-53. [PubMed:19016878] experimental

(3) Ji-Li Zhao et al. (2018) Viability, biofilm formation, and MazEF expression in drug-sensitive and drug-resistant Mycobacterium tuberculosis strains circulating in Xinjiang, China. Infection And Drug Resistance 11:345-358. [PubMed:29563815] experimental

(4) Melissa V Ramirez et al. (2013) MazF6 toxin of Mycobacterium tuberculosis demonstrates antitoxin specificity and is coupled to regulation of cell growth by a Soj-like protein. BMC Microbiology 13:240. [PubMed:24172039] experimental

(5) Holly R Ramage et al. (2009) Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genetics 5(12):e1000767. [PubMed:20011113] experimental

(6) Longxuan Zhao et al. (2008) Biochemical characterization of a chromosomal toxin-antitoxin system in Mycobacterium tuberculosis. FEBS Letters 582(5):710-4. [PubMed:18258191] experimental

(7) Soumyanetra Chandra et al. (2021) Rapid Identification of Secondary Structure and Binding Site Residues in an Intrinsically Disordered Protein Segment. Frontiers in Genetics 12:755292. [PubMed:34795695] experimental


experimental experimental literature