Detailed information of TA system TA000084

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/RHH-MazF
• Location: 754685..755225
• Replicon: chromosome
• Accession: NC_000962
• Organism: Mycobacterium tuberculosis H37Rv

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P9WII1
• Locus tag: Rv0659c
• Protein ID: NP_215173.1
• Coordinates: 754685..754993 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: O06779
• Locus tag: Rv0660c
• Protein ID: NP_215174.1
• Coordinates: 754980..755225 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Rv0654	750000..751505	+	1506	NP_215168.1	carotenoid cleavage oxygenase	-
Rv0655	751517..752596	+	1080	NP_215169.1	ABC transporter ATP-binding protein	-
Rv0656c	752984..753367	-	384	NP_215170.1	ribonuclease VapC6	-
Rv0657c	753462..753617	-	156	NP_215171.1	antitoxin VapB6	-
Rv0658c	753693..754409	-	717	NP_215172.1	integral membrane protein	-
Rv0659c	754685..754993	-	309	NP_215173.1	toxin MazF2	Toxin
Rv0660c	754980..755225	-	246	NP_215174.1	antitoxin MazE2	Antitoxin
Rv0661c	755335..755772	-	438	NP_215175.1	ribonuclease VapC7	-
Rv0662c	755769..756023	-	255	NP_215176.2	antitoxin VapB7	-
Rv0663	756137..758500	+	2364	NP_215177.1	arylsulfatase AtsD	-
Rv0664	758532..758804	+	273	NP_215178.1	antitoxin VapB8	-
Rv0665	758801..759139	+	339	NP_215179.1	ribonuclease VapC8	-
Rv0666	759136..759309	+	174	NP_215180.1	membrane protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-101)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11320.10 Da
• Isoelectric Point: 11.6554

>T84 NP_215173.1 NC_000962:c754993-754685 [Mycobacterium tuberculosis H37Rv]
MRRGELWFAATPGGDRPVLVLTRDPVADRIGAVVVVALTRTRRGLVSELELTAVENRVPSDCVVNFDNIHTLPRTAFRRR
ITRLSPARLHEACQTLRASTGC

Nucleotide

• Length: 309 bp

>T84 NC_000962:c754993-754685 [Mycobacterium tuberculosis H37Rv]
ATGCGGCGCGGTGAATTGTGGTTTGCCGCCACACCTGGTGGTGACAGACCAGTACTTGTCCTTACCAGAGATCCGGTGGC
AGACCGCATCGGCGCGGTCGTTGTGGTGGCCCTAACCCGCACCCGCCGAGGCCTGGTGTCGGAATTGGAGCTCACGGCCG
TCGAAAACCGTGTTCCGAGCGACTGCGTCGTCAACTTCGACAACATTCATACGTTGCCACGCACCGCATTCCGACGCCGC
ATCACCCGGCTGTCCCCGGCCCGCCTGCACGAAGCCTGTCAAACACTCCGGGCGAGCACGGGGTGTTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9169.01 Da
• Isoelectric Point: 4.2070

>AT84 NP_215174.1 NC_000962:c755225-754980 [Mycobacterium tuberculosis H37Rv]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Nucleotide

• Length: 246 bp

>AT84 NC_000962:c755225-754980 [Mycobacterium tuberculosis H37Rv]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T101	Mycobacterium tuberculosis H37Rv	32.673	99.02	0.324
T6247	Mycobacterium tuberculosis H37Rv	29.907	100	0.314
T655	Leptospira interrogans serovar Lai str. 56601	28.07	100	0.314

Multiple sequence alignment

Structures

Toxin

• AlphaFold DB: P9WII1

Antitoxin

• AlphaFold DB: O06779

References

(1) Holly R Ramage et al. (2009) Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genetics 5(12):e1000767. [PubMed:20011113]


 experimental literature