Detailed information of TA system TA006252

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/RHH-MazF
• Location: 3110167..3110737
• Replicon: chromosome
• Accession: NC_000962
• Organism: Mycobacterium tuberculosis H37Rv

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: Rv2801c
• Protein ID: NP_217317.1
• Coordinates: 3110167..3110523 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: Rv2801A
• Protein ID: YP_007411507.1
• Coordinates: 3110507..3110737 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Rv2797c	3105619..3107307	-	1689	NP_217313.1	hypothetical protein	-
Rv2798c	3107311..3107637	-	327	NP_217314.1	hypothetical protein	-
Rv2799	3107768..3108397	+	630	NP_217315.1	membrane protein	-
Rv2800	3108416..3110065	+	1650	NP_217316.1	hydrolase	-
Rv2801c	3110167..3110523	-	357	NP_217317.1	mRNA interferase MazF9	Toxin
Rv2801A	3110507..3110737	-	231	YP_007411507.1	antitoxin MazE9	Antitoxin
Rv2802c	3110780..3111823	-	1044	NP_217318.1	arginine/hypothetical protein	-
Rv2803	3111822..3112289	+	468	YP_177678.1	hypothetical protein	-
Rv2804c	3112465..3113094	-	630	NP_217320.1	hypothetical protein	-
Rv2805	3112867..3113271	+	405	NP_217321.1	hypothetical protein	-
Rv2806	3113268..3113459	+	192	NP_217322.1	membrane protein	-
Rv2807	3113658..3114812	+	1155	NP_217323.1	hypothetical protein	-
Rv2808	3115046..3115303	+	258	NP_217324.1	hypothetical protein	-
Rv2809	3115408..3115719	+	312	NP_217325.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T6252 NP_217317.1 NC_000962:c3110523-3110167 [Mycobacterium tuberculosis H37Rv]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T6252 NC_000962:c3110523-3110167 [Mycobacterium tuberculosis H37Rv]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT6252 YP_007411507.1 NC_000962:c3110737-3110507 [Mycobacterium tuberculosis H37Rv]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT6252 NC_000962:c3110737-3110507 [Mycobacterium tuberculosis H37Rv]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T10131	Legionella pneumophila subsp. pneumophila str. Lorraine	36.522	100	0.396
T10042	Candidatus Desulforudis audaxviator MP104C	37.391	97.458	0.364
T6247	Mycobacterium tuberculosis H37Rv	35.043	100	0.36
T1499	Bacillus anthracis str. Sterne	34.188	100	0.345
T134	Bacillus subtilis subsp. subtilis str. 168	33.333	100	0.336
T6248	Mycobacterium tuberculosis H37Rv	34.211	96.61	0.331
T6136	Lactobacillus rhamnosus Lc 705	31.356	100	0.314
T6140	Lactobacillus rhamnosus GG	31.356	100	0.314
T6141	Lactobacillus rhamnosus ATCC 8530	31.356	100	0.314
T6135	Lactobacillus rhamnosus GG	31.356	100	0.314
T10096	Clostridium difficile R20291	31.356	100	0.314
T655	Leptospira interrogans serovar Lai str. 56601	31.25	99.115	0.31
T1044	Escherichia coli str. K-12 substr. MG1655	40.964	74.775	0.306
T10130	Escherichia coli str. K-12 substr. MC4100 strain K-12	40.964	74.775	0.306
T911	Nitrosomonas europaea ATCC 19718	29.31	100	0.301

Multiple sequence alignment

Structures

Toxin

• PDB: 6L29
• PDB: 6L2A
• PDB: 7DU5
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6KYS

Antitoxin

• PDB: 6KYT
• PDB: 7DU5
• AlphaFold DB: P0CL61

References

(1) Ran Chen et al. (2021) Mechanistic Insight into the Peptide Binding Modes to Two M. tb MazF Toxins. Toxins 13(5):319. [PubMed:33925254]

(2) Holly R Ramage et al. (2009) Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genetics 5(12):e1000767. [PubMed:20011113]

(3) Amita Gupta (2009) Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. FEMS Microbiology Letters 290(1):45-53. [PubMed:19016878]

(4) Ran Chen et al. (2020) Conserved Conformational Changes in the Regulation of Mycobacterium tuberculosis MazEF-mt1. ACS Infectious Diseases 6(7):1783-1795. [PubMed:32485099]

(5) Ji-Li Zhao et al. (2018) Viability, biofilm formation, and MazEF expression in drug-sensitive and drug-resistant Mycobacterium tuberculosis strains circulating in Xinjiang, China. Infection And Drug Resistance 11:345-358. [PubMed:29563815]


 experimental literature
 protein structure literature