Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/RHH-MazF |
| Location | 2320831..2321467 | Replicon | chromosome |
| Accession | NC_000962 | ||
| Organism | Mycobacterium tuberculosis H37Rv | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | P0CL62 |
| Locus tag | Rv2063A | Protein ID | YP_004837055.2 |
| Coordinates | 2321057..2321467 (+) | Length | 137 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | P9WJ85 |
| Locus tag | Rv2063 | Protein ID | YP_177657.1 |
| Coordinates | 2320831..2321064 (+) | Length | 78 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Rv2060 | 2316279..2316680 | + | 402 | NP_216576.1 | integral membrane protein | - |
| Rv2061c | 2316681..2317085 | - | 405 | NP_216577.1 | hypothetical protein | - |
| Rv2062c | 2317169..2320753 | - | 3585 | NP_216578.1 | cobalamin biosynthesis protein CobN | - |
| Rv2063 | 2320831..2321064 | + | 234 | YP_177657.1 | antitoxin MazE7 | Antitoxin |
| Rv2063A | 2321057..2321467 | + | 411 | YP_004837055.2 | mRNA interferase MazF7 | Toxin |
| Rv2064 | 2321451..2322542 | + | 1092 | NP_216580.1 | precorrin-3B synthase | - |
| Rv2065 | 2322552..2323178 | + | 627 | NP_216581.1 | precorrin-8X methylmutase | - |
| Rv2066 | 2323175..2324701 | + | 1527 | NP_216582.1 | bifunctional S-adenosyl-L-methionine-precorrin-2 methyl transferase/precorrin-3 methylase | - |
| Rv2067c | 2324647..2325870 | - | 1224 | NP_216583.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 137 a.a. Molecular weight: 14235.42 Da Isoelectric Point: 10.6228
>T6248 YP_004837055.2 NC_000962:2321057-2321467 [Mycobacterium tuberculosis H37Rv]
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRGV
RAVARARLVERLGALKPATMRAIENALTLILGLPTGPERGEAATHSPVRWTGGRDP
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRGV
RAVARARLVERLGALKPATMRAIENALTLILGLPTGPERGEAATHSPVRWTGGRDP
Download Length: 411 bp
>T6248 NC_000962:2321057-2321467 [Mycobacterium tuberculosis H37Rv]
TTGGCTGAGCCACGGCGAGGAGACCTTTGGCTGGTCAGCCTCGGCGCCGCTCGCGCGGGTGAGCCCGGCAAGCATCGGCC
CGCGGTGGTCGTTTCCGTGGACGAGCTACTCACCGGAATCGACGACGAACTCGTTGTCGTCGTGCCGGTGTCAAGCTCGC
GCTCCCGCACCCCACTCCGGCCACCTGTCGCGCCCTCAGAAGGTGTAGCTGCCGATAGCGTCGCGGTGTGCCGCGGCGTC
CGCGCGGTCGCTCGTGCCCGACTCGTGGAGCGACTCGGCGCCCTCAAACCCGCCACGATGCGCGCAATCGAAAACGCCCT
GACCCTGATCCTCGGCCTCCCGACGGGACCTGAGCGCGGCGAGGCGGCGACCCATTCTCCCGTACGGTGGACGGGTGGCC
GGGACCCGTGA
TTGGCTGAGCCACGGCGAGGAGACCTTTGGCTGGTCAGCCTCGGCGCCGCTCGCGCGGGTGAGCCCGGCAAGCATCGGCC
CGCGGTGGTCGTTTCCGTGGACGAGCTACTCACCGGAATCGACGACGAACTCGTTGTCGTCGTGCCGGTGTCAAGCTCGC
GCTCCCGCACCCCACTCCGGCCACCTGTCGCGCCCTCAGAAGGTGTAGCTGCCGATAGCGTCGCGGTGTGCCGCGGCGTC
CGCGCGGTCGCTCGTGCCCGACTCGTGGAGCGACTCGGCGCCCTCAAACCCGCCACGATGCGCGCAATCGAAAACGCCCT
GACCCTGATCCTCGGCCTCCCGACGGGACCTGAGCGCGGCGAGGCGGCGACCCATTCTCCCGTACGGTGGACGGGTGGCC
GGGACCCGTGA
Antitoxin
Download Length: 78 a.a. Molecular weight: 8450.25 Da Isoelectric Point: 4.7954
>AT6248 YP_177657.1 NC_000962:2320831-2321064 [Mycobacterium tuberculosis H37Rv]
MSTSTTIRVSTQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG
MSTSTTIRVSTQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG
Download Length: 234 bp
>AT6248 NC_000962:2320831-2321064 [Mycobacterium tuberculosis H37Rv]
ATGTCTACATCCACGACGATTAGGGTTTCAACCCAGACTCGGGATCGTCTGGCCGCCCAAGCCCGCGAACGGGGAATCTC
GATGTCGGCTCTGCTCACCGAACTGGCCGCCCAGGCCGAGCGCCAGGCAATCTTCCGCGCCGAACGCGAGGCCTCGCACG
CCGAGACGACCACCCAGGCAGTCCGCGACGAGGACCGCGAGTGGGAGGGCACGGTAGGCGACGGCCTTGGCTGA
ATGTCTACATCCACGACGATTAGGGTTTCAACCCAGACTCGGGATCGTCTGGCCGCCCAAGCCCGCGAACGGGGAATCTC
GATGTCGGCTCTGCTCACCGAACTGGCCGCCCAGGCCGAGCGCCAGGCAATCTTCCGCGCCGAACGCGAGGCCTCGCACG
CCGAGACGACCACCCAGGCAGTCCGCGACGAGGACCGCGAGTGGGAGGGCACGGTAGGCGACGGCCTTGGCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10131 | Legionella pneumophila subsp. pneumophila str. Lorraine |
33.036 |
100 |
0.349 |
| T10042 | Candidatus Desulforudis audaxviator MP104C |
34.746 |
100 |
0.347 |
| T101 | Mycobacterium tuberculosis H37Rv |
33.333 |
100 |
0.339 |
| T6252 | Mycobacterium tuberculosis H37Rv |
34.211 |
96.61 |
0.331 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
References
(1) Ran Chen et al. (2021) Mechanistic Insight into the Peptide Binding Modes to Two M. tb MazF Toxins. Toxins 13(5):319. [PubMed:33925254]
(2) Amita Gupta (2009) Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. FEMS Microbiology Letters 290(1):45-53. [PubMed:19016878]
(3) Holly R Ramage et al. (2009) Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genetics 5(12):e1000767. [PubMed:20011113]
(4) Ran Chen et al. (2019) Structural and Biochemical Characterization of the Cognate and Heterologous Interactions of the MazEF-mt9 TA System. ACS Infectious Diseases 5(8):1306-1316. [PubMed:31267737]