Detailed information of TA system TA001044

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/AbrB-MazF
• Location: 2908778..2909361
• Replicon: chromosome
• Accession: NC_000913
• Organism: Escherichia coli str. K-12 substr. MG1655

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P0AE70
• Locus tag: b2782
• Protein ID: NP_417262.1
• Coordinates: 2910756..2911091 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0AE72
• Locus tag: b2783
• Protein ID: NP_417263.1
• Coordinates: 2911091..2911339 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
b2777	2904747..2905418	-	672	NP_417257.1	putative 7-carboxy-7-deazaguanine synthase QueE	-
b4682	2905557..2905697	+	141	YP_002791252.1	cell envelope stress response protein YqcG	-
b2778	2905711..2906583	+	873	NP_417258.4	TPM domain-containing protein YgcG	-
b2779	2906643..2907941	-	1299	NP_417259.1	enolase	-
b2780	2908029..2909666	-	1638	NP_417260.1	CTP synthetase	-
b2781	2909894..2910685	-	792	NP_417261.1	nucleoside triphosphate pyrophosphohydrolase	-
b2782	2910756..2911091	-	336	NP_417262.1	endoribonuclease toxin MazF	Toxin
b2783	2911091..2911339	-	249	NP_417263.1	antitoxin of the MazF-MazE toxin-antitoxin system MazE	Antitoxin
b2784	2911417..2913651	-	2235	NP_417264.1	GDP/GTP pyrophosphokinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12098.04 Da
• Isoelectric Point: 8.2618

>T1044 NP_417262.1 NC_000913:c2911091-2910756 [Escherichia coli str. K-12 substr. MG1655]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T1044 NC_000913:c2911091-2910756 [Escherichia coli str. K-12 substr. MG1655]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9355.74 Da
• Isoelectric Point: 4.4665

>AT1044 NP_417263.1 NC_000913:c2911339-2911091 [Escherichia coli str. K-12 substr. MG1655]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT1044 NC_000913:c2911339-2911091 [Escherichia coli str. K-12 substr. MG1655]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T10130	Escherichia coli str. K-12 substr. MC4100 strain K-12	100	100	1
T137	Deinococcus radiodurans R1	45.536	100	0.459
T10069	Klebsiella pneumoniae subsp. pneumoniae MGH 78578	44.545	99.099	0.441
T10042	Candidatus Desulforudis audaxviator MP104C	37.736	95.495	0.36
T1049	Escherichia coli str. K-12 substr. MG1655	38.835	92.793	0.36
T6120	Xylella fastidiosa	36.364	91.667	0.333
T10067	Agrobacterium tumefaciens str. C58	38.947	85.586	0.333
T6252	Mycobacterium tuberculosis H37Rv	40.964	74.775	0.306

Multiple sequence alignment

Antitoxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
AT10130	Escherichia coli str. K-12 substr. MC4100 strain K-12	100	100	1
AT137	Deinococcus radiodurans R1	41.975	100	0.425
AT1049	Escherichia coli str. K-12 substr. MG1655	32.927	100	0.329
AT10069	Klebsiella pneumoniae subsp. pneumoniae MGH 78578	36.923	85.526	0.316
AT1076	Shigella flexneri 2b	39.062	78.049	0.305

Multiple sequence alignment

Structures

Toxin

• PDB: 1UB4
• PDB: 5CQX
• PDB: 5CK9
• PDB: 5CKF
• PDB: 3NFC
• PDB: 5CKH
• PDB: 5CR2
• PDB: 5CO7
• PDB: 5CKB
• PDB: 5CQY
• PDB: 5CKD
• PDB: 5CKE

Antitoxin

• AlphaFold DB: P0AE72

References

(1) Cyrielle I Kint et al. (2012) The Escherichia coli GTPase ObgE modulates hydroxyl radical levels in response to DNA replication fork arrest. The FEBS Journal 279(19):3692-3704. [PubMed:22863262]

(2) Annabel S Lemma et al. (2021) Toxin Induction or Inhibition of Transcription or Translation Posttreatment Increases Persistence to Fluoroquinolones. MBio 12(4):e0198321. [PubMed:34399616]

(3) Toomas Mets et al. (2019) Fragmentation of Escherichia coli mRNA by MazF and MqsR. Biochimie 156:79-91. [PubMed:30315853]

(4) Nela Nikolic et al. (2018) Autoregulation of mazEF expression underlies growth heterogeneity in bacterial populations. Nucleic Acids Research 46(6):2918-2931. [PubMed:29432616]

(5) Nela Nikolic et al. (2017) MazF activation promotes translational heterogeneity of the grcA mRNA in Escherichia coli populations. PeerJ 5:e3830. [PubMed:28948108]


 experimental literature