Detailed information of TA system TA001049

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/AbrB-MazF
• Location: 4446470..4447065
• Replicon: chromosome
• Accession: NC_000913
• Organism: Escherichia coli str. K-12 substr. MG1655

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: P33647
• Locus tag: b4225
• Protein ID: NP_418646.1
• Coordinates: 4448692..4449042 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: P08365
• Locus tag: b4224
• Protein ID: NP_418645.2
• Coordinates: 4448447..4448698 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
b4219	4441538..4442176	-	639	NP_418640.1	methionine sulfoxide reductase A	-
b4220	4442382..4444115	+	1734	NP_418641.1	translocation and assembly module subunit TamA	-
b4221	4444112..4447891	+	3780	NP_418642.1	translocation and assembly module subunit TamB	-
b4222	4447894..4448235	+	342	NP_418643.1	gamma-glutamylamine cyclotransferase family protein YtfP	-
b4224	4448447..4448698	+	252	NP_418645.2	ChpS antitoxin of the ChpB-ChpS toxin-antitoxin system	Antitoxin
b4225	4448692..4449042	+	351	NP_418646.1	endoribonuclease toxin ChpB	Toxin
b4226	4449122..4449652	-	531	NP_418647.1	inorganic pyrophosphatase	-
b4227	4449962..4450918	+	957	NP_418648.1	galactofuranose ABC transporter periplasmic binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12492.40 Da
• Isoelectric Point: 5.5572

>T1049 NP_418646.1 NC_000913:4448692-4449042 [Escherichia coli str. K-12 substr. MG1655]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLHARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T1049 NC_000913:4448692-4449042 [Escherichia coli str. K-12 substr. MG1655]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9271.58 Da
• Isoelectric Point: 4.4201

>AT1049 NP_418645.2 NC_000913:4448447-4448698 [Escherichia coli str. K-12 substr. MG1655]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT1049 NC_000913:4448447-4448698 [Escherichia coli str. K-12 substr. MG1655]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T1076	Shigella flexneri 2b	43.86	98.276	0.431
T10069	Klebsiella pneumoniae subsp. pneumoniae MGH 78578	37.615	97.321	0.366
T6120	Xylella fastidiosa	42.391	85.185	0.361
T1044	Escherichia coli str. K-12 substr. MG1655	38.835	92.793	0.36
T10130	Escherichia coli str. K-12 substr. MC4100 strain K-12	38.835	92.793	0.36
T137	Deinococcus radiodurans R1	37.383	92.241	0.345
T1499	Bacillus anthracis str. Sterne	33.929	96.552	0.328
T10067	Agrobacterium tumefaciens str. C58	38.947	81.897	0.319
T134	Bacillus subtilis subsp. subtilis str. 168	33.333	95.69	0.319
T663	Streptococcus mutans UA159	30.357	100	0.309

Multiple sequence alignment

Antitoxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
AT1076	Shigella flexneri 2b	37.179	93.976	0.349
AT10130	Escherichia coli str. K-12 substr. MC4100 strain K-12	32.927	100	0.329
AT1044	Escherichia coli str. K-12 substr. MG1655	32.927	100	0.329
AT10067	Agrobacterium tumefaciens str. C58	32.5	96.386	0.313
AT10069	Klebsiella pneumoniae subsp. pneumoniae MGH 78578	38.983	77.632	0.303

Multiple sequence alignment

Structures

Toxin

• AlphaFold DB: P33647

Antitoxin

• AlphaFold DB: P08365

References

(1) Monique B Kamphuis et al. (2006) Model for RNA binding and the catalytic site of the RNase Kid of the bacterial parD toxin-antitoxin system. Journal of Molecular Biology 357(1):115-26. [PubMed:16413033]

(2) Susanne K Christensen et al. (2003) Toxin-antitoxin loci as stress-response-elements: ChpAK/MazF and ChpBK cleave translated RNAs and are counteracted by tmRNA. Journal of Molecular Biology 332(4):809-19. [PubMed:12972253]

(3) Y Masuda et al. (1993) chpA and chpB, Escherichia coli chromosomal homologs of the pem locus responsible for stable maintenance of plasmid R100. Journal of Bacteriology 175(21):6850-6. [PubMed:8226627]

(4) Maria Belitsky et al. (2011) The Escherichia coli extracellular death factor EDF induces the endoribonucleolytic activities of the toxins MazF and ChpBK. Molecular Cell 41(6):625-35. [PubMed:21419338]


 experimental literature