Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/- |
| Location | 928967..929592 | Replicon | chromosome |
| Accession | NC_003062 | ||
| Organism | Agrobacterium tumefaciens str. C58 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | Q7D0B1 |
| Locus tag | ATU_RS04645 | Protein ID | WP_006311244.1 |
| Coordinates | 929233..929592 (+) | Length | 120 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | Q7D0B2 |
| Locus tag | ATU_RS04640 | Protein ID | WP_010971270.1 |
| Coordinates | 928967..929233 (+) | Length | 89 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ATU_RS04610 (AGR_C_1701) | 924141..924476 | - | 336 | WP_010971265.1 | type II toxin-antitoxin system YafQ family toxin | - |
| ATU_RS04615 (AGR_C_1704) | 924473..924748 | - | 276 | WP_010971266.1 | type II toxin-antitoxin system RelB/DinJ family antitoxin | - |
| ATU_RS04620 (AGR_C_1706) | 924856..925464 | + | 609 | WP_010971267.1 | class I SAM-dependent methyltransferase | - |
| ATU_RS04625 (AGR_C_1708) | 925640..928225 | + | 2586 | WP_035256421.1 | heavy metal translocating P-type ATPase | - |
| ATU_RS04630 (AGR_C_1709) | 928222..928644 | + | 423 | WP_006311241.1 | Cu(I)-responsive transcriptional regulator | - |
| ATU_RS04635 | 928697..928897 | + | 201 | WP_006311242.1 | heavy-metal-associated domain-containing protein | - |
| ATU_RS04640 (AGR_C_1711) | 928967..929233 | + | 267 | WP_010971270.1 | AbrB/MazE/SpoVT family DNA-binding domain-containing protein | Antitoxin |
| ATU_RS04645 (AGR_C_1712) | 929233..929592 | + | 360 | WP_006311244.1 | type II toxin-antitoxin system PemK/MazF family toxin | Toxin |
| ATU_RS04650 | 929600..929848 | - | 249 | WP_006311245.1 | hypothetical protein | - |
| ATU_RS04655 (AGR_C_1716) | 930027..931415 | + | 1389 | WP_169539085.1 | MFS transporter | - |
| ATU_RS26520 (AGR_C_1719) | 931494..931667 | - | 174 | WP_010971273.1 | hypothetical protein | - |
| ATU_RS04660 (AGR_C_1721) | 931736..933454 | - | 1719 | WP_035256424.1 | glycoside hydrolase family 32 protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 120 a.a. Molecular weight: 12857.04 Da Isoelectric Point: 8.2782
>T10067 WP_006311244.1 NC_003062:929233-929592 [Agrobacterium fabrum str. C58]
MVRNQIPKRGDVYLVDLNPVVGSEIKDEHRCVVITPREINAVGLCLVVPVTTGGMFTRKAGLAVNISGHKTTGVALCNQV
RSMDIVARVAQKKAKYIETLDDATIDEIAGRVISMIDPA
MVRNQIPKRGDVYLVDLNPVVGSEIKDEHRCVVITPREINAVGLCLVVPVTTGGMFTRKAGLAVNISGHKTTGVALCNQV
RSMDIVARVAQKKAKYIETLDDATIDEIAGRVISMIDPA
Download Length: 360 bp
>T10067 NC_003062:929233-929592 [Agrobacterium fabrum str. C58]
ATGGTCCGCAACCAGATCCCCAAGCGCGGCGACGTTTATCTGGTTGATCTAAACCCTGTCGTAGGCAGCGAAATCAAGGA
CGAACATCGCTGTGTCGTCATCACGCCCAGAGAAATTAACGCGGTCGGACTCTGTCTCGTCGTCCCGGTGACCACCGGCG
GCATGTTTACGCGCAAGGCAGGGCTTGCCGTAAATATATCCGGCCACAAGACAACGGGCGTCGCTTTGTGCAATCAGGTG
AGAAGCATGGATATCGTCGCCCGGGTTGCCCAGAAGAAAGCGAAATATATCGAAACCCTCGATGATGCGACGATCGATGA
AATCGCCGGGCGCGTCATCAGCATGATCGATCCAGCTTGA
ATGGTCCGCAACCAGATCCCCAAGCGCGGCGACGTTTATCTGGTTGATCTAAACCCTGTCGTAGGCAGCGAAATCAAGGA
CGAACATCGCTGTGTCGTCATCACGCCCAGAGAAATTAACGCGGTCGGACTCTGTCTCGTCGTCCCGGTGACCACCGGCG
GCATGTTTACGCGCAAGGCAGGGCTTGCCGTAAATATATCCGGCCACAAGACAACGGGCGTCGCTTTGTGCAATCAGGTG
AGAAGCATGGATATCGTCGCCCGGGTTGCCCAGAAGAAAGCGAAATATATCGAAACCCTCGATGATGCGACGATCGATGA
AATCGCCGGGCGCGTCATCAGCATGATCGATCCAGCTTGA
Antitoxin
Download Length: 89 a.a. Molecular weight: 9423.02 Da Isoelectric Point: 5.1106
>AT10067 WP_010971270.1 NC_003062:928967-929233 [Agrobacterium fabrum str. C58]
MTVTTKIRRQGGAAVMTIPPALLKMLGLEIGEQLTLEVDNGALVASPVRLEKKRFTLAELLDGAEEVAALNARERAWDTA
PPVGKEAL
MTVTTKIRRQGGAAVMTIPPALLKMLGLEIGEQLTLEVDNGALVASPVRLEKKRFTLAELLDGAEEVAALNARERAWDTA
PPVGKEAL
Download Length: 267 bp
>AT10067 NC_003062:928967-929233 [Agrobacterium fabrum str. C58]
ATGACCGTGACCACGAAAATAAGACGCCAGGGCGGCGCGGCGGTGATGACGATCCCGCCCGCTCTTTTGAAGATGCTGGG
GCTGGAAATCGGTGAGCAACTGACGCTCGAGGTCGATAATGGCGCTCTGGTGGCAAGCCCGGTTCGACTGGAAAAGAAAC
GCTTCACGCTGGCCGAATTACTGGATGGTGCAGAGGAAGTGGCAGCTCTAAACGCCAGGGAGCGGGCGTGGGACACAGCG
CCTCCTGTCGGCAAAGAAGCGTTGTGA
ATGACCGTGACCACGAAAATAAGACGCCAGGGCGGCGCGGCGGTGATGACGATCCCGCCCGCTCTTTTGAAGATGCTGGG
GCTGGAAATCGGTGAGCAACTGACGCTCGAGGTCGATAATGGCGCTCTGGTGGCAAGCCCGGTTCGACTGGAAAAGAAAC
GCTTCACGCTGGCCGAATTACTGGATGGTGCAGAGGAAGTGGCAGCTCTAAACGCCAGGGAGCGGGCGTGGGACACAGCG
CCTCCTGTCGGCAAAGAAGCGTTGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T6120 | Xylella fastidiosa |
36.036 |
100 |
0.37 |
| T1076 | Shigella flexneri 2b |
37.5 |
94.118 |
0.353 |
| T10130 | Escherichia coli str. K-12 substr. MC4100 strain K-12 |
38.947 |
85.586 |
0.333 |
| T1044 | Escherichia coli str. K-12 substr. MG1655 |
38.947 |
85.586 |
0.333 |
| T10069 | Klebsiella pneumoniae subsp. pneumoniae MGH 78578 |
32.143 |
100 |
0.321 |
| T1049 | Escherichia coli str. K-12 substr. MG1655 |
38.947 |
81.897 |
0.319 |
| T134 | Bacillus subtilis subsp. subtilis str. 168 |
32.727 |
94.828 |
0.31 |
| T10090 | Enterococcus faecalis ATCC 29212 |
30.631 |
98.23 |
0.301 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT6120 | Xylella fastidiosa |
42.353 |
97.701 |
0.414 |
| AT1076 | Shigella flexneri 2b |
38.824 |
100 |
0.388 |
| AT1049 | Escherichia coli str. K-12 substr. MG1655 |
32.5 |
96.386 |
0.313 |
Multiple sequence alignment
References
(1) Wonho Choi et al. (2021) Functional Characterization of the mazEF Toxin-Antitoxin System in the Pathogenic Bacterium Agrobacterium tumefaciens. Microorganisms 9(5):1107. [PubMed:34065548]