Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) mazEF/-
Location 3872442..3873025 Replicon chromosome
Accession NZ_HG738867
Organism Escherichia coli str. K-12 substr. MC4100 strain K-12

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag BN896_RS19135 Protein ID WP_000254738.1
Coordinates 3872690..3873025 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag BN896_RS19130 Protein ID WP_000581937.1
Coordinates 3872442..3872690 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BN896_RS19105 (BN896_2523) 3867445..3868746 + 1302 WP_000046812.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
BN896_RS19110 (BN896_2522) 3868794..3869054 + 261 Protein_3621 GTP diphosphokinase -
BN896_RS19120 (BN896_2520) 3869145..3870373 + 1229 WP_088895425.1 IS3-like element IS2 family transposase -
BN896_RS19125 (BN896_2519) 3870385..3872364 + 1980 Protein_3623 GTP diphosphokinase -
BN896_RS19130 (BN896_2518) 3872442..3872690 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
BN896_RS19135 (BN896_2517) 3872690..3873025 + 336 WP_000254738.1 endoribonuclease MazF Toxin
BN896_RS19140 (BN896_2516) 3873096..3873887 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
BN896_RS19145 (BN896_2515) 3874115..3875752 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
BN896_RS19150 (BN896_2514) 3875840..3877138 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T10130 WP_000254738.1 NZ_HG738867:3872690-3873025 [Escherichia coli str. K-12 substr. MC4100]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T10130 NZ_HG738867:3872690-3873025 [Escherichia coli str. K-12 substr. MC4100]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT10130 WP_000581937.1 NZ_HG738867:3872442-3872690 [Escherichia coli str. K-12 substr. MC4100]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT10130 NZ_HG738867:3872442-3872690 [Escherichia coli str. K-12 substr. MC4100]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T1044 Escherichia coli str. K-12 substr. MG1655

100

100

1
T137 Deinococcus radiodurans R1

45.536

100

0.459
T10069 Klebsiella pneumoniae subsp. pneumoniae MGH 78578

44.545

99.099

0.441
T10042 Candidatus Desulforudis audaxviator MP104C

37.736

95.495

0.36
T1049 Escherichia coli str. K-12 substr. MG1655

38.835

92.793

0.36
T6120 Xylella fastidiosa

36.364

91.667

0.333
T10067 Agrobacterium tumefaciens str. C58

38.947

85.586

0.333
T6252 Mycobacterium tuberculosis H37Rv

40.964

74.775

0.306

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT1044 Escherichia coli str. K-12 substr. MG1655

100

100

1
AT137 Deinococcus radiodurans R1

41.975

100

0.425
AT1049 Escherichia coli str. K-12 substr. MG1655

32.927

100

0.329
AT10069 Klebsiella pneumoniae subsp. pneumoniae MGH 78578

36.923

85.526

0.316
AT1076 Shigella flexneri 2b

39.062

78.049

0.305

Multiple sequence alignment    




Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References

(1) Akanksha Nigam et al. (2019) Stress-Induced MazF-Mediated Proteins in Escherichia coli. MBio 10(2):e00340-19. [PubMed:30914510] experimental


experimental experimental literature