Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/- |
| Location | 1433..1998 | Replicon | plasmid pKPN7 |
| Accession | NC_009653 | ||
| Organism | Klebsiella pneumoniae subsp. pneumoniae MGH 78578 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | A6TJ73 |
| Locus tag | KPN_RS28325 | Protein ID | WP_011977836.1 |
| Coordinates | 1660..1998 (+) | Length | 113 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | A6TJ72 |
| Locus tag | KPN_RS28320 | Protein ID | WP_011977835.1 |
| Coordinates | 1433..1663 (+) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KPN_RS28315 (KPN_pKPN7p10262) | 564..1241 | - | 678 | WP_117058735.1 | helix-turn-helix domain-containing protein | - |
| KPN_RS28320 (KPN_pKPN7p10263) | 1433..1663 | + | 231 | WP_011977835.1 | AbrB/MazE/SpoVT family DNA-binding domain-containing protein | Antitoxin |
| KPN_RS28325 (KPN_pKPN7p10264) | 1660..1998 | + | 339 | WP_011977836.1 | endoribonuclease MazF | Toxin |
| KPN_RS28330 (KPN_pKPN7p10265) | 2667..2858 | + | 192 | WP_011977837.1 | Rop family plasmid primer RNA-binding protein | - |
| KPN_RS28335 (KPN_pKPN7p10266) | 2858..3259 | + | 402 | WP_011977838.1 | transcriptional regulator | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| inside | Non-Mobilizable plasmid | - | - | 1..3478 | 3478 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 113 a.a. Molecular weight: 11979.70 Da Isoelectric Point: 5.7830
>T10069 WP_011977836.1 NC_009653:1660-1998 [Klebsiella pneumoniae subsp. pneumoniae MGH 78578]
VTTYCPARGDVILLDFNPQSGHEQAGKRPALVVSDDLFNQVTGFAVVCPITNQIKGYPFEVPVDGTTKTTGVILADQVKS
LDWKARAARTVDSVSGETVTTVVDMVSKIIKA
VTTYCPARGDVILLDFNPQSGHEQAGKRPALVVSDDLFNQVTGFAVVCPITNQIKGYPFEVPVDGTTKTTGVILADQVKS
LDWKARAARTVDSVSGETVTTVVDMVSKIIKA
Download Length: 339 bp
>T10069 NC_009653:1660-1998 [Klebsiella pneumoniae subsp. pneumoniae MGH 78578]
GTGACGACATATTGTCCAGCCAGAGGCGATGTCATTCTTTTAGATTTTAACCCTCAATCAGGGCATGAGCAGGCAGGGAA
AAGGCCTGCTCTTGTCGTTTCTGATGATTTGTTTAACCAAGTCACTGGGTTTGCTGTTGTTTGCCCCATAACTAATCAGA
TAAAAGGGTACCCTTTTGAAGTTCCTGTTGATGGGACTACTAAAACAACAGGGGTTATCTTGGCTGATCAAGTGAAGTCT
TTAGACTGGAAGGCCAGAGCGGCACGTACTGTTGATAGCGTTTCCGGCGAGACAGTAACTACAGTTGTTGATATGGTTTC
AAAAATTATTAAAGCATAA
GTGACGACATATTGTCCAGCCAGAGGCGATGTCATTCTTTTAGATTTTAACCCTCAATCAGGGCATGAGCAGGCAGGGAA
AAGGCCTGCTCTTGTCGTTTCTGATGATTTGTTTAACCAAGTCACTGGGTTTGCTGTTGTTTGCCCCATAACTAATCAGA
TAAAAGGGTACCCTTTTGAAGTTCCTGTTGATGGGACTACTAAAACAACAGGGGTTATCTTGGCTGATCAAGTGAAGTCT
TTAGACTGGAAGGCCAGAGCGGCACGTACTGTTGATAGCGTTTCCGGCGAGACAGTAACTACAGTTGTTGATATGGTTTC
AAAAATTATTAAAGCATAA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8399.68 Da Isoelectric Point: 6.4459
>AT10069 WP_011977835.1 NC_009653:1433-1663 [Klebsiella pneumoniae subsp. pneumoniae MGH 78578]
MTTAIGKWGNGAALRIPQPFMKQLGLSIGDVVNFRVTERDLVITKAGPTLEELLGQCTAENRHHEYLCDSQGKEML
MTTAIGKWGNGAALRIPQPFMKQLGLSIGDVVNFRVTERDLVITKAGPTLEELLGQCTAENRHHEYLCDSQGKEML
Download Length: 231 bp
>AT10069 NC_009653:1433-1663 [Klebsiella pneumoniae subsp. pneumoniae MGH 78578]
ATGACTACGGCTATTGGTAAATGGGGTAATGGCGCTGCTTTGCGTATCCCGCAGCCGTTCATGAAGCAGCTTGGGCTTAG
TATCGGTGATGTTGTTAATTTCCGCGTCACTGAAAGGGATTTAGTAATTACTAAAGCTGGCCCGACGCTTGAAGAACTGC
TCGGACAGTGCACTGCAGAAAATCGCCATCACGAGTATCTATGTGACTCTCAAGGTAAGGAGATGCTGTGA
ATGACTACGGCTATTGGTAAATGGGGTAATGGCGCTGCTTTGCGTATCCCGCAGCCGTTCATGAAGCAGCTTGGGCTTAG
TATCGGTGATGTTGTTAATTTCCGCGTCACTGAAAGGGATTTAGTAATTACTAAAGCTGGCCCGACGCTTGAAGAACTGC
TCGGACAGTGCACTGCAGAAAATCGCCATCACGAGTATCTATGTGACTCTCAAGGTAAGGAGATGCTGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T1044 | Escherichia coli str. K-12 substr. MG1655 |
44.545 |
99.099 |
0.441 |
| T10130 | Escherichia coli str. K-12 substr. MC4100 strain K-12 |
44.545 |
99.099 |
0.441 |
| T137 | Deinococcus radiodurans R1 |
40.909 |
98.214 |
0.402 |
| T6120 | Xylella fastidiosa |
45.556 |
83.333 |
0.38 |
| T1049 | Escherichia coli str. K-12 substr. MG1655 |
37.615 |
97.321 |
0.366 |
| T1076 | Shigella flexneri 2b |
37.383 |
95.536 |
0.357 |
| T10090 | Enterococcus faecalis ATCC 29212 |
34.906 |
94.643 |
0.33 |
| T6142 | Lactobacillus rhamnosus ATCC 8530 |
38.71 |
83.036 |
0.321 |
| T6137 | Lactobacillus rhamnosus Lc 705 |
38.71 |
83.036 |
0.321 |
| T6238 | Lactobacillus rhamnosus ATCC 8530 |
38.71 |
83.036 |
0.321 |
| T10042 | Candidatus Desulforudis audaxviator MP104C |
36.735 |
87.5 |
0.321 |
| T10067 | Agrobacterium tumefaciens str. C58 |
32.143 |
100 |
0.321 |
| T134 | Bacillus subtilis subsp. subtilis str. 168 |
34 |
89.286 |
0.304 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT137 | Deinococcus radiodurans R1 |
38.235 |
89.474 |
0.342 |
| AT1852 | Lactobacillus salivarius UCC118 |
33.333 |
100 |
0.342 |
| AT1044 | Escherichia coli str. K-12 substr. MG1655 |
36.923 |
85.526 |
0.316 |
| AT10130 | Escherichia coli str. K-12 substr. MC4100 strain K-12 |
36.923 |
85.526 |
0.316 |
| AT6120 | Xylella fastidiosa |
35.385 |
85.526 |
0.303 |
| AT1049 | Escherichia coli str. K-12 substr. MG1655 |
38.983 |
77.632 |
0.303 |
Multiple sequence alignment
References
(1) Chenglong Jin et al. (2021) Structural and functional analysis of the Klebsiella pneumoniae MazEF toxin-antitoxin system. IUCrJ 8(Pt 3):362-371. [PubMed:33953923]