Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | pemIK/AbrB-MazF |
| Location | 18177..18778 | Replicon | plasmid pSF118-20 |
| Accession | NC_006529 | ||
| Organism | Lactobacillus salivarius UCC118 | ||
Toxin (Protein)
| Gene name | pemK | Uniprot ID | U6FEM0 |
| Locus tag | LSL_RS00130 | Protein ID | WP_011241744.1 |
| Coordinates | 18434..18778 (+) | Length | 115 a.a. |
Antitoxin (Protein)
| Gene name | pemI | Uniprot ID | A0A806J926 |
| Locus tag | LSL_RS00125 | Protein ID | WP_001748109.1 |
| Coordinates | 18177..18440 (+) | Length | 88 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LSL_RS00085 (LSL_1977) | 13518..14090 | - | 573 | WP_011241737.1 | hypothetical protein | - |
| LSL_RS00090 | 14241..14345 | + | 105 | WP_041823142.1 | putative holin-like toxin | - |
| LSL_RS00095 (LSL_1978) | 14418..15797 | - | 1380 | WP_011241738.1 | IS3 family transposase | - |
| LSL_RS00100 (LSL_1979) | 16061..16240 | + | 180 | Protein_20 | UV-resistance | - |
| LSL_RS00105 (LSL_1980) | 16362..16766 | + | 405 | WP_164920212.1 | LysM peptidoglycan-binding domain-containing protein | - |
| LSL_RS00110 (LSL_1981) | 16759..17211 | + | 453 | WP_011241741.1 | hypothetical protein | - |
| LSL_RS00120 (LSL_1983) | 17833..18090 | - | 258 | WP_011241743.1 | hypothetical protein | - |
| LSL_RS00125 (LSL_1984) | 18177..18440 | + | 264 | WP_001748109.1 | AbrB/MazE/SpoVT family DNA-binding domain-containing protein | Antitoxin |
| LSL_RS00130 (LSL_1985) | 18434..18778 | + | 345 | WP_011241744.1 | type II toxin-antitoxin system PemK/MazF family toxin | Toxin |
| LSL_RS00135 | 18868..18930 | - | 63 | WP_143455695.1 | hypothetical protein | - |
| LSL_RS00140 (LSL_1986) | 19002..20222 | + | 1221 | WP_011241745.1 | IS256 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| inside | Non-Mobilizable plasmid | - | - | 1..20417 | 20417 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 115 a.a. Molecular weight: 13010.79 Da Isoelectric Point: 5.5556
>T1852 WP_011241744.1 NC_006529:18434-18778 [Lactobacillus salivarius UCC118]
MVSQGDIFYVNFNPSRGHEQMNRRPAIALSNDLVCQTSNMTIVAPISSTERNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVADEAIVDHVSREELEEIITLYKLLFSIDDK
MVSQGDIFYVNFNPSRGHEQMNRRPAIALSNDLVCQTSNMTIVAPISSTERNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVADEAIVDHVSREELEEIITLYKLLFSIDDK
Download Length: 345 bp
>T1852 NC_006529:18434-18778 [Lactobacillus salivarius UCC118]
ATGGTAAGTCAGGGCGATATATTCTATGTTAACTTTAATCCAAGCCGTGGTCATGAACAGATGAATAGGCGGCCAGCCAT
TGCTCTAAGCAATGATCTAGTCTGTCAGACAAGTAACATGACGATTGTGGCCCCAATTAGCAGTACCGAGCGGAATTTTC
CAATGTATCATCGACTAACTAGTAGTCAAACAGTATATGGAAAAGTATTATTAGATCAAACAATAGCTTTAGATTTGCGG
GCAAGACATGTCGCTGACGAAGCTATTGTCGATCATGTATCGCGTGAAGAATTAGAAGAAATAATCACTCTGTACAAATT
ATTGTTTAGTATTGATGACAAATAA
ATGGTAAGTCAGGGCGATATATTCTATGTTAACTTTAATCCAAGCCGTGGTCATGAACAGATGAATAGGCGGCCAGCCAT
TGCTCTAAGCAATGATCTAGTCTGTCAGACAAGTAACATGACGATTGTGGCCCCAATTAGCAGTACCGAGCGGAATTTTC
CAATGTATCATCGACTAACTAGTAGTCAAACAGTATATGGAAAAGTATTATTAGATCAAACAATAGCTTTAGATTTGCGG
GCAAGACATGTCGCTGACGAAGCTATTGTCGATCATGTATCGCGTGAAGAATTAGAAGAAATAATCACTCTGTACAAATT
ATTGTTTAGTATTGATGACAAATAA
Antitoxin
Download Length: 88 a.a. Molecular weight: 10141.47 Da Isoelectric Point: 5.7117
>AT1852 WP_001748109.1 NC_006529:18177-18440 [Lactobacillus salivarius UCC118]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWQDDGKRDHELDWGKS
EGNELQW
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWQDDGKRDHELDWGKS
EGNELQW
Download Length: 264 bp
>AT1852 NC_006529:18177-18440 [Lactobacillus salivarius UCC118]
ATGGAACTTATTAAAGAACAAACACGCCTGGCAAAGTGGGGCAACTCGAAAGCTGCTAGAATTCCTAGCCAGATTATTAA
ACAATTGAAATTAGATGATAACCAAGATATGACAATAACAATTGAAAATGGATCGATTGTTTTAACACCAATAAAAAAGA
ATCCGACTAATATTCACGAACTATTTAAGGACTGGCAAGATGACGGGAAACGTGATCACGAGCTTGATTGGGGAAAGTCA
GAAGGTAATGAACTTCAATGGTAA
ATGGAACTTATTAAAGAACAAACACGCCTGGCAAAGTGGGGCAACTCGAAAGCTGCTAGAATTCCTAGCCAGATTATTAA
ACAATTGAAATTAGATGATAACCAAGATATGACAATAACAATTGAAAATGGATCGATTGTTTTAACACCAATAAAAAAGA
ATCCGACTAATATTCACGAACTATTTAAGGACTGGCAAGATGACGGGAAACGTGATCACGAGCTTGATTGGGGAAAGTCA
GAAGGTAATGAACTTCAATGGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T663 | Streptococcus mutans UA159 |
39.815 |
98.182 |
0.391 |
| T6091 | Staphylococcus aureus |
31.776 |
95.536 |
0.304 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT10069 | Klebsiella pneumoniae subsp. pneumoniae MGH 78578 |
33.333 |
100 |
0.342 |
| AT10101 | Nitrospira sp. ND1 isolate Nitrospira sp. strain ND1 |
30 |
100 |
0.303 |
Multiple sequence alignment
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | U6FEM0 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A806J926 |
References
(1) Fang Fang et al. (2008) Characterization of endogenous plasmids from Lactobacillus salivarius UCC118. Applied And Environmental Microbiology 74(10):3216-28. [PubMed:18390685]