Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | pemIK/AbrB-MazF |
| Location | 14508..15112 | Replicon | plasmid pCH91 |
| Accession | NC_020227 | ||
| Organism | Staphylococcus aureus | ||
Toxin (Protein)
| Gene name | pemK | Uniprot ID | L7PFJ6 |
| Locus tag | HS951_RS00090 | Protein ID | WP_001022435.1 |
| Coordinates | 14508..14846 (-) | Length | 113 a.a. |
Antitoxin (Protein)
| Gene name | pemI | Uniprot ID | - |
| Locus tag | HS951_RS00095 | Protein ID | WP_237342570.1 |
| Coordinates | 14843..15226 (-) | Length | 128 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HS951_RS00070 (SACH_a14) | 10096..10875 | + | 780 | WP_015379483.1 | replication initiator protein A | - |
| HS951_RS00075 (SACH_a15) | 10872..11546 | - | 675 | WP_015379484.1 | IS6 family transposase | - |
| HS951_RS00080 (SACH_a16) | 11762..12922 | - | 1161 | WP_015379485.1 | aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme | - |
| HS951_RS00085 (SACH_a17) | 13650..14333 | - | 684 | WP_000277644.1 | type II CAAX endopeptidase family protein | - |
| HS951_RS00090 (SACH_a18) | 14508..14846 | - | 339 | WP_001022435.1 | type II toxin-antitoxin system PemK/MazF family toxin | Toxin |
| HS951_RS00095 (SACH_a19) | 14843..15226 | - | 384 | WP_237342570.1 | hypothetical protein | Antitoxin |
| HS951_RS00100 (SACH_a20) | 15683..16492 | + | 810 | WP_015379486.1 | alpha/beta hydrolase | - |
| HS951_RS00105 (SACH_a21) | 16605..17336 | + | 732 | WP_015379487.1 | CPBP family intramembrane glutamic endopeptidase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| inside | Non-Mobilizable plasmid | - | - | 1..17515 | 17515 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 113 a.a. Molecular weight: 13001.20 Da Isoelectric Point: 10.1988
>T6091 WP_001022435.1 NC_020227:c14846-14508 [Staphylococcus aureus]
MNIKQFDILYIDLNPTRGREKHNVRPCLVINNQMSIDGTNFVWVLPITTRGLRYPTDIQLKTKKGLVSGVIDTVQIRALD
LKARQYNYKDELQDNLKNDILKAIKTYLKPTL
MNIKQFDILYIDLNPTRGREKHNVRPCLVINNQMSIDGTNFVWVLPITTRGLRYPTDIQLKTKKGLVSGVIDTVQIRALD
LKARQYNYKDELQDNLKNDILKAIKTYLKPTL
Download Length: 339 bp
>T6091 NC_020227:c14846-14508 [Staphylococcus aureus]
ATGAACATTAAACAATTTGACATACTCTACATTGATTTAAATCCAACTCGTGGACGTGAAAAGCATAACGTAAGGCCCTG
CTTAGTGATAAATAATCAGATGAGTATTGATGGCACTAATTTTGTTTGGGTTTTACCCATAACAACTCGGGGTTTACGTT
ATCCAACAGATATCCAACTTAAAACAAAAAAAGGATTAGTATCTGGTGTGATAGACACTGTTCAAATACGCGCTTTAGAC
CTAAAAGCACGTCAATACAACTATAAAGACGAACTACAGGACAATTTAAAAAATGATATATTAAAAGCGATAAAAACTTA
TCTTAAGCCAACGTTATAA
ATGAACATTAAACAATTTGACATACTCTACATTGATTTAAATCCAACTCGTGGACGTGAAAAGCATAACGTAAGGCCCTG
CTTAGTGATAAATAATCAGATGAGTATTGATGGCACTAATTTTGTTTGGGTTTTACCCATAACAACTCGGGGTTTACGTT
ATCCAACAGATATCCAACTTAAAACAAAAAAAGGATTAGTATCTGGTGTGATAGACACTGTTCAAATACGCGCTTTAGAC
CTAAAAGCACGTCAATACAACTATAAAGACGAACTACAGGACAATTTAAAAAATGATATATTAAAAGCGATAAAAACTTA
TCTTAAGCCAACGTTATAA
Antitoxin
Download Length: 128 a.a. Molecular weight: 14584.02 Da Isoelectric Point: 10.4411
>AT6091 WP_237342570.1 NC_020227:c15226-14843 [Staphylococcus aureus]
MQSRIVKAMFLIFINYSTSDVFTLVTTFVMMNKRGLIVMAKSLKDLNKTSERVFKNGNSKAISLSKKTIRLANFDIGDTV
EVQKMNGGLFITKKKESIEDRIKNFFQSGGKYTELEVDWEERVGREI
MQSRIVKAMFLIFINYSTSDVFTLVTTFVMMNKRGLIVMAKSLKDLNKTSERVFKNGNSKAISLSKKTIRLANFDIGDTV
EVQKMNGGLFITKKKESIEDRIKNFFQSGGKYTELEVDWEERVGREI
Download Length: 384 bp
>AT6091 NC_020227:c15226-14843 [Staphylococcus aureus]
ATGCAGTCGAGGATAGTAAAAGCGATGTTTTTGATTTTTATAAATTATTCAACTTCAGATGTATTTACTTTGGTAACTAC
ATTTGTTATGATGAACAAAAGGGGGCTGATAGTAATGGCTAAATCATTAAAAGACTTAAACAAGACTTCGGAACGAGTTT
TTAAAAATGGAAACAGTAAAGCAATCAGTTTAAGTAAAAAAACTATCAGGTTAGCTAATTTTGATATAGGAGATACAGTT
GAAGTTCAAAAAATGAATGGTGGTTTGTTCATTACTAAAAAAAAGGAGTCTATTGAAGATAGAATTAAAAATTTCTTTCA
AAGTGGTGGAAAGTATACAGAATTAGAAGTAGATTGGGAAGAAAGAGTTGGCCGAGAAATATGA
ATGCAGTCGAGGATAGTAAAAGCGATGTTTTTGATTTTTATAAATTATTCAACTTCAGATGTATTTACTTTGGTAACTAC
ATTTGTTATGATGAACAAAAGGGGGCTGATAGTAATGGCTAAATCATTAAAAGACTTAAACAAGACTTCGGAACGAGTTT
TTAAAAATGGAAACAGTAAAGCAATCAGTTTAAGTAAAAAAACTATCAGGTTAGCTAATTTTGATATAGGAGATACAGTT
GAAGTTCAAAAAATGAATGGTGGTTTGTTCATTACTAAAAAAAAGGAGTCTATTGAAGATAGAATTAAAAATTTCTTTCA
AAGTGGTGGAAAGTATACAGAATTAGAAGTAGATTGGGAAGAAAGAGTTGGCCGAGAAATATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T6120 | Xylella fastidiosa |
31.481 |
100 |
0.315 |
| T1852 | Lactobacillus salivarius UCC118 |
31.776 |
95.536 |
0.304 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT10101 | Nitrospira sp. ND1 isolate Nitrospira sp. strain ND1 |
31.818 |
100 |
0.321 |
Multiple sequence alignment
References
(1) Michal Bukowski et al. (2013) A regulatory role for Staphylococcus aureus toxin-antitoxin system PemIKSa. Nature Communications 4:2012. [PubMed:23774061]
(2) Do-Hee Kim et al. (2022) Role of PemI in the Staphylococcus aureus PemIK toxin-antitoxin complex: PemI controls PemK by acting as a PemK loop mimic. Nucleic Acids Research 50(4):2319-2333. [PubMed:35141752]