211433

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF-doc
Location 12358..12962 Replicon plasmid pNV_1
Accession NZ_CP080250
Organism Staphylococcus aureus strain NV_1

Toxin (Protein)


Gene name pemK Uniprot ID L7PFJ6
Locus tag KZW86_RS14925 Protein ID WP_001022435.1
Coordinates 12624..12962 (+) Length 113 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID L7PH55
Locus tag KZW86_RS14920 Protein ID WP_001129937.1
Coordinates 12358..12627 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZW86_RS14905 (KZW86_14810) 9462..9830 + 369 WP_041203784.1 hypothetical protein -
KZW86_RS14910 (KZW86_14815) 10134..10865 - 732 WP_000753929.1 CPBP family intramembrane metalloprotease -
KZW86_RS14915 (KZW86_14820) 10978..11787 - 810 WP_015379486.1 alpha/beta hydrolase -
KZW86_RS14920 (KZW86_14825) 12358..12627 + 270 WP_001129937.1 hypothetical protein Antitoxin
KZW86_RS14925 (KZW86_14830) 12624..12962 + 339 WP_001022435.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KZW86_RS14930 (KZW86_14835) 13137..13820 + 684 WP_000277644.1 type II CAAX endopeptidase family protein -
KZW86_RS14935 (KZW86_14840) 14399..15073 + 675 WP_001106066.1 IS6 family transposase -
KZW86_RS14940 (KZW86_14845) 15225..15635 - 411 WP_000872163.1 hypothetical protein -
KZW86_RS14945 (KZW86_14850) 15645..15878 - 234 WP_001002995.1 hypothetical protein -
KZW86_RS14950 (KZW86_14855) 15910..17193 - 1284 WP_000083517.1 replication initiator protein A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Non-Mobilizable plasmid - - 1..17257 17257
- flank IS/Tn - - 14399..15073 674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 113 a.a.        Molecular weight: 13001.20 Da        Isoelectric Point: 10.1988

>T211433 WP_001022435.1 NZ_CP080250:12624-12962 [Staphylococcus aureus]
MNIKQFDILYIDLNPTRGREKHNVRPCLVINNQMSIDGTNFVWVLPITTRGLRYPTDIQLKTKKGLVSGVIDTVQIRALD
LKARQYNYKDELQDNLKNDILKAIKTYLKPTL

Download         Length: 339 bp

>T211433 NZ_CP104861:3773541-3774425 [Pseudomonas fragi]
ATGCTCCACGCCACCGATCATCTAACCCCGACGCTGACCGCCGACCTTGAAGCAAGGCTGGCCGTTAACTTCCCCCTGCG
CACGCTGGACGTCGCAGGCGGGCGTCAAAGCTTGCGCGAATGCGGGAGCGGGCCGGCCATTGTGCTGTTGCACGGTATTG
GCTCTGGCGCGGCGTCCTGGTTGGCGGTGGCGCAGTTGCTGGCGCAACGCGTACGGGTAATTGCCTGGGATGCCCCCGGC
TACGGGGAGTCCAGCCCGCTACGTTCGCTGGCCCCGGATGCATCCGACTACGCCGAGCGCCTGCGGCAAATGCTCGACGC
ACTGGATATTGAGCGTTGTGTGCTGGTGGGCCATTCCCTTGGTGCAATCACGGCAACGGCCTTCGCCAGCGGCGCACAGC
AACAACGGGTAAGCCGACTGGTGTTGATCAGCCCGGCCCAGGGCTATGGCCATCCATCGCGCAAGATTCAGGGCCAGGCC
GTGCGTCTAAAACGCCTGGCCGACCTCGAATACCCAGGCATTCAGGCCATGGCCGAGCAACGCAGTGCCTACATGCTTTC
ACCCGGCGCCAGCCCCAAGGATCACGCCTGGGTGCGCTGGAACATGGCGCGGCTTAACCCCGAGGGTTATCGCCAGGCCA
TTGAACTGCTGTGCGGCGATGACCTGCTGCGCCACCCCCCGCTGTCGATGCCGTGCGACGTGCATTGCGGCGCCGCCGAC
GGCATCACCACACCCGACAGCTGCCGGGTACTGGCCCAGACCTTGGGCGCCAGCTTCAGCCTGATTGACAGCGCAGGACA
TGCCAGCCCTATCGAACAACCCTATGCGGTGGCAGGCCAACTGGCCATTGCCATCAAACAATCCCTGACAGGTGCATCGC
TATGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10185.65 Da        Isoelectric Point: 10.1641

>AT211433 WP_001129937.1 NZ_CP080250:12358-12627 [Staphylococcus aureus]
MAKSLKDLNKTSERVFKNGNSKAISLSKKTIRLANFDIGDTVEVQKMNGGLFITKKKESIEDRIKNFFQSGGKYTELEVD
WEERVGREI

Download         Length: 270 bp

>AT211433 NZ_CP104861:3774422-3775258 [Pseudomonas fragi]
ATGACAGATTCAGAACTGCCCAAGGACGCTCAGGACAAATATATCGTGCCGGGCCTGGAGCGCGGCTTGCTGCTGCTGTG
CGAGTTCACGCGCAGCAACCGCACCCTGACCGCCCCCGAGCTGGCGCGGCGTCTGGCCCTGCCGCGCTCGACGATTTTTC
GTCTGCTGACCACCCTTGAAACCATGGGTTTCGTCACCCGCAGCGGCAACGAATACCGCCTCGGCATGTCGGTACTGCGC
CTGGGCTTCGAGTACCTGGCCTCGCTGGAACTGACCGAGCTGGGGCAACCGTTGCTCGCGCGTTTGTGTGACCGGGTCAA
CTATCCGAGCAACCTGGTGGTCCGCGATGGCCGCTCCATCGTGTACGTGGCCAAGGTCTCGCCACCGTCGGTGTTCTCCA
GCGCAGTCAACGTCGGCACCCGCCTGCCCGCACACGCCACGGTGCTGGGGCGCATCCTGCTGGAAGACCTGTCACTGAGC
GAATTACGCGAGCTGTTCCCCGAAGAACATCTTGAACAGCACTCCCCCTGCACGCCAAAAACCGCCCTGGAACTGTTCGA
CATGGTACAGGCAGACCGCGAGCGCGGTTTCGTCAGCGGCGAAGGTTTCTTCGAGTCGTCGATCTCCACCGTTGCCGCTC
CGGTGCGCGATGAGTCAAGCAGGATTGTTGCCGCCCTGGGGGTGACCATTCCCACCGCGCAGATTGCCCACGTCAACTTC
AACGAGTTGCTGCTGCAAGTGCGCCGCAGCGCCGACGAGCTGTCGCGGTTGCTCAACTACAACAGCGGCGCGGCCAACGA
TGGCCACAACCGCGTCACCGCCTTGATGAGGGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7EWJ
PDB 7EWI

Antitoxin

Source ID Structure
PDB 7EWJ
AlphaFold DB L7PH55

References