134861

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF-doc
Location 5806..6410 Replicon plasmid pSALNCL17
Accession NZ_CP042045
Organism Staphylococcus aureus strain B2-15A

Toxin (Protein)


Gene name pemK Uniprot ID L7PFJ6
Locus tag FP478_RS14295 Protein ID WP_001022435.1
Coordinates 5806..6144 (-) Length 113 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID L7PH55
Locus tag FP478_RS14300 Protein ID WP_001129937.1
Coordinates 6141..6410 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FP478_RS14270 1575..2858 + 1284 WP_000083517.1 replication initiator protein A -
FP478_RS14275 2890..3123 + 234 WP_001002995.1 hypothetical protein -
FP478_RS14280 3133..3543 + 411 WP_114306678.1 hypothetical protein -
FP478_RS14285 3695..4369 - 675 WP_001106066.1 IS6 family transposase -
FP478_RS14290 4948..5631 - 684 WP_000277644.1 CPBP family intramembrane metalloprotease -
FP478_RS14295 5806..6144 - 339 WP_001022435.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FP478_RS14300 6141..6410 - 270 WP_001129937.1 hypothetical protein Antitoxin
FP478_RS14305 6981..7790 + 810 WP_001106667.1 alpha/beta hydrolase -
FP478_RS14310 7903..8634 + 732 WP_000753929.1 CPBP family intramembrane metalloprotease -
FP478_RS14315 8938..9306 - 369 WP_041203784.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Non-Mobilizable plasmid - - 1..17035 17035
- flank IS/Tn - - 3695..4369 674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 113 a.a.        Molecular weight: 13001.20 Da        Isoelectric Point: 10.1988

>T134861 WP_001022435.1 NZ_CP042045:c6144-5806 [Staphylococcus aureus]
MNIKQFDILYIDLNPTRGREKHNVRPCLVINNQMSIDGTNFVWVLPITTRGLRYPTDIQLKTKKGLVSGVIDTVQIRALD
LKARQYNYKDELQDNLKNDILKAIKTYLKPTL

Download         Length: 339 bp

>T134861 NZ_CP042045:c6144-5806 [Staphylococcus aureus]
ATGAACATTAAACAATTTGACATACTCTACATTGATTTAAATCCAACTCGTGGACGTGAAAAGCATAACGTAAGGCCCTG
CTTAGTGATAAATAATCAGATGAGTATTGATGGCACTAATTTTGTTTGGGTTTTACCCATAACAACTCGGGGTTTACGTT
ATCCAACAGATATCCAACTTAAAACAAAAAAAGGATTAGTATCTGGTGTGATAGACACTGTTCAAATACGCGCTTTAGAC
CTAAAAGCACGTCAATACAACTATAAAGACGAACTACAGGACAATTTAAAAAATGATATATTAAAAGCGATAAAAACTTA
TCTTAAGCCAACGTTATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10185.65 Da        Isoelectric Point: 10.1641

>AT134861 WP_001129937.1 NZ_CP042045:c6410-6141 [Staphylococcus aureus]
MAKSLKDLNKTSERVFKNGNSKAISLSKKTIRLANFDIGDTVEVQKMNGGLFITKKKESIEDRIKNFFQSGGKYTELEVD
WEERVGREI

Download         Length: 270 bp

>AT134861 NZ_CP042045:c6410-6141 [Staphylococcus aureus]
ATGGCTAAATCATTAAAAGACTTAAACAAGACTTCGGAACGAGTTTTTAAAAATGGAAACAGTAAAGCAATCAGTTTAAG
TAAAAAAACTATCAGGTTAGCTAATTTTGATATAGGAGATACAGTTGAAGTTCAAAAAATGAATGGTGGTTTGTTCATTA
CTAAAAAAAAGGAGTCTATTGAAGATAGAATTAAAAATTTCTTTCAAAGTGGTGGAAAGTATACAGAATTAGAAGTAGAT
TGGGAAGAAAGAGTTGGCCGAGAAATATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7EWJ
PDB 7EWI

Antitoxin

Source ID Structure
PDB 7EWJ
AlphaFold DB L7PH55

References