Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/AbrB-MazF |
| Location | 2484225..2484787 | Replicon | chromosome |
| Accession | NC_013199 | ||
| Organism | Lactobacillus rhamnosus Lc 705 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | C2JYM4 |
| Locus tag | LC705_RS11555 | Protein ID | WP_005692155.1 |
| Coordinates | 2484225..2484572 (-) | Length | 116 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | - |
| Locus tag | LC705_RS11560 | Protein ID | WP_014571589.1 |
| Coordinates | 2484572..2484787 (-) | Length | 72 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LC705_RS11535 (LC705_02438) | 2479361..2480062 | - | 702 | WP_005692159.1 | ABC transporter ATP-binding protein | - |
| LC705_RS11540 (LC705_02439) | 2480055..2480873 | - | 819 | WP_014571588.1 | ABC transporter permease subunit | - |
| LC705_RS11545 (LC705_02440) | 2481114..2481782 | - | 669 | WP_005692157.1 | tRNA ligase | - |
| LC705_RS11550 (LC705_02441) | 2482248..2483360 | - | 1113 | WP_015764265.1 | IS110 family transposase | - |
| LC705_RS15160 (LC705_02442) | 2483559..2483702 | + | 144 | WP_015764720.1 | hypothetical protein | - |
| LC705_RS11555 (LC705_02444) | 2484225..2484572 | - | 348 | WP_005692155.1 | type II toxin-antitoxin system PemK/MazF family toxin | Toxin |
| LC705_RS11560 (LC705_02445) | 2484572..2484787 | - | 216 | WP_014571589.1 | hypothetical protein | Antitoxin |
| LC705_RS11565 (LC705_02446) | 2484884..2486719 | - | 1836 | WP_015764721.1 | ABC transporter ATP-binding protein/permease | - |
| LC705_RS11570 (LC705_02447) | 2486706..2488496 | - | 1791 | WP_015764722.1 | ABC transporter ATP-binding protein/permease | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 116 a.a. Molecular weight: 13150.01 Da Isoelectric Point: 7.9817
>T6137 WP_005692155.1 NC_013199:c2484572-2484225 [Lactobacillus rhamnosus Lc 705]
MTYKAKQGDVVWLDFDPSEGHEIRKRCPAVVLSSNAYNRATGFVIVAPITSTIRTLPGYVDIVSNKIRGQVVTSQVYSLD
VTEQGNRKIDFIERMNIEDFYTVAQFTKMNFDFKF
MTYKAKQGDVVWLDFDPSEGHEIRKRCPAVVLSSNAYNRATGFVIVAPITSTIRTLPGYVDIVSNKIRGQVVTSQVYSLD
VTEQGNRKIDFIERMNIEDFYTVAQFTKMNFDFKF
Download Length: 348 bp
>T6137 NC_013199:c2484572-2484225 [Lactobacillus rhamnosus Lc 705]
ATGACATATAAGGCTAAGCAAGGTGATGTGGTGTGGCTTGATTTTGATCCGTCCGAGGGTCATGAAATTAGAAAAAGATG
CCCAGCTGTTGTTTTAAGCAGTAACGCATACAATCGTGCAACCGGATTTGTGATTGTCGCACCAATTACCTCAACAATTC
GGACACTACCTGGTTATGTTGATATTGTATCCAATAAAATTCGTGGTCAGGTTGTCACTTCGCAGGTTTATTCTCTGGAT
GTCACTGAACAAGGCAACCGAAAAATCGATTTTATTGAAAGAATGAACATTGAAGATTTTTATACAGTCGCTCAATTCAC
CAAAATGAATTTTGATTTTAAATTTTAG
ATGACATATAAGGCTAAGCAAGGTGATGTGGTGTGGCTTGATTTTGATCCGTCCGAGGGTCATGAAATTAGAAAAAGATG
CCCAGCTGTTGTTTTAAGCAGTAACGCATACAATCGTGCAACCGGATTTGTGATTGTCGCACCAATTACCTCAACAATTC
GGACACTACCTGGTTATGTTGATATTGTATCCAATAAAATTCGTGGTCAGGTTGTCACTTCGCAGGTTTATTCTCTGGAT
GTCACTGAACAAGGCAACCGAAAAATCGATTTTATTGAAAGAATGAACATTGAAGATTTTTATACAGTCGCTCAATTCAC
CAAAATGAATTTTGATTTTAAATTTTAG
Antitoxin
Download Length: 72 a.a. Molecular weight: 7986.13 Da Isoelectric Point: 5.7995
>AT6137 WP_014571589.1 NC_013199:c2484787-2484572 [Lactobacillus rhamnosus Lc 705]
MDVKVVKRGNSLALSLPSSAGFKRGEAFLLISDEKGGYLLIPKVKNPYTKAKPLSFHQEEAWPDFDYEDIE
MDVKVVKRGNSLALSLPSSAGFKRGEAFLLISDEKGGYLLIPKVKNPYTKAKPLSFHQEEAWPDFDYEDIE
Download Length: 216 bp
>AT6137 NC_013199:c2484787-2484572 [Lactobacillus rhamnosus Lc 705]
ATGGATGTGAAAGTTGTAAAACGTGGAAACTCGTTAGCTTTATCACTTCCGAGCAGTGCGGGTTTCAAACGAGGCGAGGC
GTTTTTGCTGATTTCAGATGAAAAAGGTGGTTATCTGTTGATCCCAAAGGTAAAAAATCCCTATACCAAAGCAAAACCAT
TATCATTCCATCAAGAAGAAGCTTGGCCGGATTTTGACTATGAGGATATTGAATAA
ATGGATGTGAAAGTTGTAAAACGTGGAAACTCGTTAGCTTTATCACTTCCGAGCAGTGCGGGTTTCAAACGAGGCGAGGC
GTTTTTGCTGATTTCAGATGAAAAAGGTGGTTATCTGTTGATCCCAAAGGTAAAAAATCCCTATACCAAAGCAAAACCAT
TATCATTCCATCAAGAAGAAGCTTGGCCGGATTTTGACTATGAGGATATTGAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T6238 | Lactobacillus rhamnosus ATCC 8530 |
100 |
100 |
1 |
| T6142 | Lactobacillus rhamnosus ATCC 8530 |
100 |
100 |
1 |
| T10090 | Enterococcus faecalis ATCC 29212 |
42.727 |
97.345 |
0.416 |
| T10069 | Klebsiella pneumoniae subsp. pneumoniae MGH 78578 |
38.71 |
83.036 |
0.321 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT6238 | Lactobacillus rhamnosus ATCC 8530 |
100 |
100 |
1 |
| AT6142 | Lactobacillus rhamnosus ATCC 8530 |
100 |
100 |
1 |
| AT137 | Deinococcus radiodurans R1 |
30.137 |
100 |
0.31 |
Multiple sequence alignment
References
(1) K M Klimina et al. (2013) Identification and characterization of toxin-antitoxin systems in strains of Lactobacillus rhamnosus isolated from humans. Anaerobe 22:82-9. [PubMed:23727113]