76394

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK(toxin)
Location 96231..96771 Replicon 1 (D-7475) plasmid unnamed1
Accession NZ_CP021284
Organism Legionella pneumophila subsp. pneumophila strain Allentown

Toxin (Protein)


Gene name mazF Uniprot ID A0A7G4RL43
Locus tag CA921_RS16015 Protein ID WP_014845117.1
Coordinates 96231..96551 (-) Length 107 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A2H5FRI5
Locus tag CA921_RS16020 Protein ID WP_014845118.1
Coordinates 96538..96771 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA921_RS15985 91243..91545 - 303 WP_106229768.1 transposase -
CA921_RS15990 92171..92662 - 492 WP_014845113.1 Hsp20/alpha crystallin family protein -
CA921_RS15995 92810..93397 - 588 WP_014845114.1 Hsp20/alpha crystallin family protein -
CA921_RS16710 93762..93917 - 156 WP_154080667.1 hypothetical protein -
CA921_RS16000 93989..94480 + 492 WP_051009158.1 IS91 family transposase -
CA921_RS16005 94502..95035 + 534 WP_061637559.1 IS91 family transposase -
CA921_RS16010 95401..95793 + 393 Protein_100 methyltransferase domain-containing protein -
CA921_RS16015 96231..96551 - 321 WP_014845117.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CA921_RS16020 96538..96771 - 234 WP_014845118.1 hypothetical protein Antitoxin
CA921_RS16025 96880..97227 - 348 WP_080031944.1 WGR domain-containing protein -
CA921_RS16030 97333..98217 - 885 WP_014845120.1 recombinase family protein -
CA921_RS16035 98430..98690 + 261 WP_014845121.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
CA921_RS16040 98683..98964 + 282 WP_014845122.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family -
CA921_RS16045 99144..101642 - 2499 WP_014845123.1 DEAD/DEAH box helicase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Non-Mobilizable plasmid blaOXA-29 - 1..150433 150433


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 107 a.a.        Molecular weight: 11444.32 Da        Isoelectric Point: 9.4321

>T76394 WP_014845117.1 NZ_CP021284:c96551-96231 [Legionella pneumophila subsp. pneumophila]
MNRGDVYWVNLDPTTGSEINKLRPCVLVGATPINQARRTVVVVPLSSSATARPPITITVSCLGKQVTAVCDQIRTIDKSR
LKKVAGNLSDKDLNALDDGLRQVLCL

Download         Length: 321 bp

>T76394 NZ_CP021284:c96551-96231 [Legionella pneumophila subsp. pneumophila]
ATGAATCGTGGTGATGTATATTGGGTAAACCTCGACCCTACTACAGGCTCTGAAATCAACAAACTCAGACCTTGCGTTTT
AGTTGGAGCAACACCGATTAATCAAGCAAGACGCACTGTTGTCGTCGTTCCTCTTTCAAGCTCTGCTACAGCCAGACCAC
CAATTACCATCACTGTTTCTTGTCTTGGCAAACAAGTTACCGCAGTTTGTGATCAAATAAGAACGATTGATAAAAGTCGT
TTAAAAAAAGTGGCAGGAAACCTTTCAGATAAAGATTTAAATGCCTTGGATGATGGGTTACGACAAGTTTTATGCCTTTG
A

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8932.14 Da        Isoelectric Point: 4.8047

>AT76394 WP_014845118.1 NZ_CP021284:c96771-96538 [Legionella pneumophila subsp. pneumophila]
MNKVLISIPDQIASRMRAAIPQRQRSKVIAHLIEKEIERREKALYECALAVENDHGLQNEMNDWDITVQDGLTDESW

Download         Length: 234 bp

>AT76394 NZ_CP021284:c96771-96538 [Legionella pneumophila subsp. pneumophila]
ATGAATAAAGTTTTAATATCAATACCCGATCAAATCGCTTCTAGAATGAGAGCAGCAATACCTCAGCGACAACGCAGCAA
GGTGATTGCTCATCTTATTGAAAAAGAAATTGAACGACGTGAAAAAGCGCTCTATGAATGCGCTTTAGCTGTTGAAAATG
ATCATGGTTTACAAAATGAAATGAACGATTGGGATATAACGGTTCAAGATGGATTAACTGATGAATCGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7G4RL43

Antitoxin

Source ID Structure
AlphaFold DB A0A2H5FRI5

References