Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK(toxin)
Location 93111..93651 Replicon plasmid unnamed
Accession NZ_CP011106
Organism Legionella pneumophila strain L10-023 isolate Ulm

Toxin (Protein)


Gene name mazF Uniprot ID A0A7G4RL43
Locus tag ULM_RS16940 Protein ID WP_014845117.1
Coordinates 93111..93431 (-) Length 107 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A2H5FRI5
Locus tag ULM_RS16945 Protein ID WP_014845118.1
Coordinates 93418..93651 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ULM_RS16910 88124..88426 - 303 WP_014845112.1 transposase -
ULM_RS16915 89052..89543 - 492 WP_014845113.1 Hsp20/alpha crystallin family protein -
ULM_RS16920 89691..90278 - 588 WP_014845114.1 Hsp20/alpha crystallin family protein -
ULM_RS18015 90643..90798 - 156 WP_154080667.1 hypothetical protein -
ULM_RS16925 90870..91361 + 492 WP_051009158.1 IS91 family transposase -
ULM_RS16930 91383..91916 + 534 WP_061637559.1 IS91 family transposase -
ULM_RS16935 92282..92674 + 393 Protein_100 methyltransferase domain-containing protein -
ULM_RS16940 93111..93431 - 321 WP_014845117.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
ULM_RS16945 93418..93651 - 234 WP_014845118.1 hypothetical protein Antitoxin
ULM_RS16950 93759..94106 - 348 WP_080031944.1 WGR domain-containing protein -
ULM_RS16955 94212..95096 - 885 WP_014845120.1 recombinase family protein -
ULM_RS16960 95309..95569 + 261 WP_014845121.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
ULM_RS16965 95562..95843 + 282 WP_014845122.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family -
ULM_RS16970 96023..98521 - 2499 WP_014845123.1 DEAD/DEAH box helicase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Non-Mobilizable plasmid blaOXA-29 - 1..143694 143694


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 107 a.a.        Molecular weight: 11444.32 Da        Isoelectric Point: 9.4321

>T53378 WP_014845117.1 NZ_CP011106:c93431-93111 [Legionella pneumophila]
MNRGDVYWVNLDPTTGSEINKLRPCVLVGATPINQARRTVVVVPLSSSATARPPITITVSCLGKQVTAVCDQIRTIDKSR
LKKVAGNLSDKDLNALDDGLRQVLCL

Download         Length: 321 bp

>T53378 NZ_CP011106:c93431-93111 [Legionella pneumophila]
ATGAATCGTGGTGATGTATATTGGGTAAACCTCGACCCTACTACAGGCTCTGAAATCAACAAACTCAGACCTTGCGTTTT
AGTTGGAGCAACACCGATTAATCAAGCAAGACGCACTGTTGTCGTCGTTCCTCTTTCAAGCTCTGCTACAGCCAGACCAC
CAATTACCATCACTGTTTCTTGTCTTGGCAAACAAGTTACCGCAGTTTGTGATCAAATAAGAACGATTGATAAAAGTCGT
TTAAAAAAAGTGGCAGGAAACCTTTCAGATAAAGATTTAAATGCCTTGGATGATGGGTTACGACAAGTTTTATGCCTTTG
A

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8932.14 Da        Isoelectric Point: 4.8047

>AT53378 WP_014845118.1 NZ_CP011106:c93651-93418 [Legionella pneumophila]
MNKVLISIPDQIASRMRAAIPQRQRSKVIAHLIEKEIERREKALYECALAVENDHGLQNEMNDWDITVQDGLTDESW

Download         Length: 234 bp

>AT53378 NZ_CP011106:c93651-93418 [Legionella pneumophila]
ATGAATAAAGTTTTAATATCAATACCCGATCAAATCGCTTCTAGAATGAGAGCAGCAATACCTCAGCGACAACGCAGCAA
GGTGATTGCTCATCTTATTGAAAAAGAAATTGAACGACGTGAAAAAGCGCTCTATGAATGCGCTTTAGCTGTTGAAAATG
ATCATGGTTTACAAAATGAAATGAACGATTGGGATATAACGGTTCAAGATGGATTAACTGATGAATCGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7G4RL43

Antitoxin

Source ID Structure
AlphaFold DB A0A2H5FRI5

References