Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 29488..30131 Replicon plasmid pMBR_DHA-1_1CV2
Accession NZ_CP049949
Organism Klebsiella pneumoniae strain 19KM267

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag G9173_RS27350 Protein ID WP_000754566.1
Coordinates 29715..30131 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag G9173_RS27345 Protein ID WP_001261276.1
Coordinates 29488..29718 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9173_RS27310 24607..24876 + 270 WP_000339857.1 hypothetical protein -
G9173_RS27315 25053..25919 - 867 WP_004118283.1 replication initiation protein -
G9173_RS27320 26449..26553 + 105 WP_032409716.1 hypothetical protein -
G9173_RS27325 26682..26939 + 258 WP_000764642.1 hypothetical protein -
G9173_RS27330 26997..27773 - 777 WP_000015958.1 site-specific integrase -
G9173_RS27335 27770..28513 - 744 WP_000129823.1 hypothetical protein -
G9173_RS27340 28564..28914 - 351 WP_000493378.1 hypothetical protein -
G9173_RS27345 29488..29718 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
G9173_RS27350 29715..30131 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
G9173_RS27355 30335..31191 + 857 Protein_31 IS3-like element ISEc15 family transposase -
G9173_RS27360 31473..32549 + 1077 WP_000227969.1 IS110 family transposase -
G9173_RS27365 33190..33273 + 84 Protein_33 SOS response-associated peptidase -
G9173_RS27370 33436..33960 - 525 Protein_34 Tn3 family transposase -
G9173_RS27375 34025..34729 + 705 WP_001067834.1 IS6-like element IS26 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Mobilizable plasmid blaDHA-1 / sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / mph(A) - 1..43417 43417


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T151836 WP_000754566.1 NZ_CP049949:29715-30131 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T151836 NZ_CP065777:252694-252960 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT151836 WP_001261276.1 NZ_CP049949:29488-29718 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT151836 NZ_CP065777:252431-252694 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References