Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 28549..29192 Replicon plasmid pMBR_DHA-1_1CV2_18KM2517
Accession NZ_CP039940
Organism Klebsiella pneumoniae strain 18KM2517

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag FC642_RS26955 Protein ID WP_000754566.1
Coordinates 28776..29192 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag FC642_RS26950 Protein ID WP_001261276.1
Coordinates 28549..28779 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FC642_RS26915 23668..23937 + 270 WP_000339857.1 hypothetical protein -
FC642_RS26920 24114..24980 - 867 WP_004118283.1 replication initiation protein -
FC642_RS26925 25510..25614 + 105 WP_032409716.1 hypothetical protein -
FC642_RS26930 25743..26000 + 258 WP_000764642.1 hypothetical protein -
FC642_RS26935 26058..26834 - 777 WP_000015958.1 site-specific integrase -
FC642_RS26940 26831..27574 - 744 WP_000129823.1 hypothetical protein -
FC642_RS26945 27625..27975 - 351 WP_000493378.1 hypothetical protein -
FC642_RS26950 28549..28779 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FC642_RS26955 28776..29192 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
FC642_RS26960 29396..30252 + 857 Protein_32 IS3-like element ISEc15 family transposase -
FC642_RS26965 30534..31610 + 1077 WP_000227969.1 IS110 family transposase -
FC642_RS26970 32251..32334 + 84 Protein_34 SOS response-associated peptidase -
FC642_RS26975 32497..33021 - 525 Protein_35 Tn3 family transposase -
FC642_RS26980 33086..33790 + 705 WP_001067834.1 IS6-like element IS26 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Mobilizable plasmid sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / mph(A) / blaDHA-1 / qnrB4 - 1..54444 54444


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T124564 WP_000754566.1 NZ_CP039940:28776-29192 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T124564 NZ_CP039940:28776-29192 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT124564 WP_001261276.1 NZ_CP039940:28549-28779 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT124564 NZ_CP039940:28549-28779 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References