Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 28653..29296 Replicon plasmid p4_IncR
Accession NZ_CP071256
Organism Klebsiella pneumoniae strain MEI006

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag J0G26_RS28045 Protein ID WP_000754566.1
Coordinates 28880..29296 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag J0G26_RS28040 Protein ID WP_001261276.1
Coordinates 28653..28883 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J0G26_RS28005 (J0G26_27950) 23772..24041 + 270 WP_000339857.1 hypothetical protein -
J0G26_RS28010 (J0G26_27955) 24218..25084 - 867 WP_004118283.1 replication initiation protein -
J0G26_RS28015 (J0G26_27960) 25614..25718 + 105 WP_032409716.1 hypothetical protein -
J0G26_RS28020 (J0G26_27965) 25847..26104 + 258 WP_000764642.1 hypothetical protein -
J0G26_RS28025 (J0G26_27970) 26162..26938 - 777 WP_000015958.1 site-specific integrase -
J0G26_RS28030 (J0G26_27975) 26935..27678 - 744 WP_000129823.1 hypothetical protein -
J0G26_RS28035 (J0G26_27980) 27729..28079 - 351 WP_000493378.1 hypothetical protein -
J0G26_RS28040 (J0G26_27985) 28653..28883 + 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
J0G26_RS28045 (J0G26_27990) 28880..29296 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
J0G26_RS28050 (J0G26_27995) 29500..30356 + 857 Protein_32 IS3-like element ISEc15 family transposase -
J0G26_RS28055 (J0G26_28000) 30638..31714 + 1077 WP_000227969.1 IS110 family transposase -
J0G26_RS28060 (J0G26_28005) 32355..32438 + 84 Protein_34 SOS response-associated peptidase -
J0G26_RS28065 (J0G26_28010) 32601..33122 - 522 Protein_35 Tn3 family transposase -
J0G26_RS28070 (J0G26_28015) 33190..33894 + 705 WP_001067834.1 IS6-like element IS26 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Mobilizable plasmid sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / aph(3')-Ia / mph(A) / blaDHA-1 / qnrB4 - 1..56333 56333


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T196030 WP_000754566.1 NZ_CP071256:28880-29296 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T196030 NZ_CP095004:c76403-76119 [Klebsiella michiganensis]
ATGACCTATAAGTTAGCCTTCAATGAATCAGCTTTGAAAGAATGGAAAAAACTGGGTCATACTATTCGGGAGCAATTCAA
AAAGAAGCTTTCCGAACGGCTTCAGAACCCACGGGTGCCCGCAGCACAACTCCATGGAGGTAAAGACCAGTATAAAATAA
AGCTCCATGGCGCGGGTTACCGACTGGTTTACAGTGTCAACGATGAAATTGTGACGGTCACCGTAATTGGGGTGGGCAAA
CGCAACAATGATGATATCTACAACGCAACCCGACACAGAAATTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT196030 WP_001261276.1 NZ_CP071256:28653-28883 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT196030 NZ_CP095004:c76641-76393 [Klebsiella michiganensis]
ATGGCATTTCAGGTTTTAACCACCACAGCAGCCAGCATTACAGAACTTAAACGTGACCCTATGGGTACCTTCAATGCAGG
AGAAGGTGCACCGGTAGCAATCCTGAATCGCAACGAACCCGCTTTTTACTGTGTACCACCAGCCCTGTATGCTCAGCTGA
TGGAAATGCTGGAAGATCAAGAGTTGGGGCGTATTGCTGATGAACGAATCGGTGAGGACCGAATCGAGGTCAACATTGAT
GACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References