Detailed information of TA system TA196745

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2010212..2010840
• Replicon: chromosome
• Accession: NZ_CP071698
• Organism: Actinobacillus pleuropneumoniae strain SC1810

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J3K87_RS09395
• Protein ID: WP_012263372.1
• Coordinates: 2010442..2010840 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B0BSF9
• Locus tag: J3K87_RS09390
• Protein ID: WP_005616131.1
• Coordinates: 2010212..2010442 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3K87_RS09375 (J3K87_09360)	2007611..2009026	+	1416	WP_005605761.1	AAA family ATPase	-
J3K87_RS09380 (J3K87_09365)	2009019..2009591	+	573	WP_005620147.1	hypothetical protein	-
J3K87_RS09385 (J3K87_09370)	2009964..2010104	-	141	WP_005602535.1	hypothetical protein	-
J3K87_RS09390 (J3K87_09375)	2010212..2010442	+	231	WP_005616131.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
J3K87_RS09395 (J3K87_09380)	2010442..2010840	+	399	WP_012263372.1	tRNA(fMet)-specific endonuclease VapC	Toxin
J3K87_RS09400 (J3K87_09385)	2010976..2011392	-	417	WP_012263373.1	DUF417 family protein	-
J3K87_RS09405 (J3K87_09390)	2011638..2012288	+	651	WP_012263374.1	DUF2202 domain-containing protein	-
J3K87_RS09410 (J3K87_09395)	2012361..2013020	-	660	WP_005605767.1	hypothetical protein	-
J3K87_RS09415 (J3K87_09400)	2013334..2014656	-	1323	WP_012263375.1	HslU--HslV peptidase ATPase subunit	-
J3K87_RS09420 (J3K87_09405)	2014802..2015323	-	522	WP_005613306.1	ATP-dependent protease subunit HslV	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_010278 Actinobacillus pleuropneumoniae serovar 3 str. JL03 Genomic island TA004922, TA022622 1913969..1931996 0	inside	Genomic island	-	-	1994992..2013020	18028

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (6-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15077.34 Da
• Isoelectric Point: 7.4195

>T196745 WP_012263372.1 NZ_CP071698:2010442-2010840 [Actinobacillus pleuropneumoniae]
MLTYMLDTNIAIYVVKRRPIEVLDKFNLNSTRLCVSSITAAELYYGAEKSQFPERNMAVIEDFLSRLTILDYTHKAATHF
GNIKAHLSKQGKIIGENDIHIAAHARSEGLVLVTNNLREFERVEGLRLDNWV

Nucleotide

• Length: 399 bp

>T196745 NZ_CP095280:2994838-2995845 [Aeromonas hydrophila]
ATGGCCAGCCTGACGGTCTACATGAACGGATACCGGGTAGGTACCTTTACCAAGGCGACCAATGGTGCTCACCAATTTCA
GTACGATGAATCGTGGGTGAAGTTGCCAGGCAGCCGCCCCATCTCGCTCTCCATGCCATTACGCCAGCAGCGCTACCAGG
GCGATGAGGCCTATAACTTCTTCGATAATCTGCTGCCAGATAACCCGGAAGTAAGAAACCGGGTGGTGGCGCGCTATCAA
GCCGATTCCACCCAACCCTTTGATCTGCTCAGTTGCATTGGCCAGGACAGCGTGGGGGCATTGCAACTGGTGGCTCAGGG
CAGACCTGTGCCAGACGTTAAGCGCATTGAGTGCAAGCCGCTCTCTGACGCCGAACTGGAGCAGATCATGACCAGCTACC
AGCAAGGGATCCCGCTTGGTATGGTGAGGGAAGAGGACGATTTTCGGATATCCATCGCCGGGGCTCAGGAAAAGACCGCG
TTACTCTACCTCGACAACCGTTGGTGCTTACCGCATGCAGCGACCCCAACCACCCACATTATCAAGCTGCCCATTGGCAA
GATCGAAAGCCACTCCTACTCGATAGACCTCTCCCAGAGCGTAGAAAACGAATATCTCTGCACCCTGATCGCCAAAGCAT
TGGGTCTTCCGGTTCCCCATTGCTTCATCATGCAGGTCGGCAAGGTCAAGGCTCTGGCGGTGGAACGCTTCGATCGGCGC
TACGCCACGGATCGCAGTTGGATCATGCGCCTGCCCCAAGAGGACTTCTGCCAGGTGTTGAATGTACCGTCAGCACGCAA
GTACGAGAGCCATGGTGGACCGGGCATTGCTGACATCATGACGACCCTGCTGGGATCGGCCACGCCGGAGCAGGATCGGT
ACCTGTTTATGAAGTCTCAGGTGTTGTTCTGGCTGCTGGCTGCCACTGATGCATATTCCCACGCATGGCGATCGCTCAAT
AACATTCATGCCGATCACGTAATAACATCCATGCCGATCACTCAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8595.68 Da
• Isoelectric Point: 4.5358

>AT196745 WP_005616131.1 NZ_CP071698:2010212-2010442 [Actinobacillus pleuropneumoniae]
MEASVFMTNRSQAVRLPAEVRFASDVKKLSVRVVGNERILAPINQSWDSFFLGSATVSDDFMTEREISTQAEREEL

Nucleotide

• Length: 231 bp

>AT196745 NZ_CP095280:2994584-2994838 [Aeromonas hydrophila]
ATGAAAGTAACCAGCGCCAAGCAACTTAGTGCCTGTCTCAAGGATGCCAGGCTTTCGCAAAAGCTCTCCCAAGGCAAAGT
GGCCGGCAAGGTGGGGATCAGGCAGGACACGGTGTCCAGCTTCGAGCTCAATCCCGAGTCAACCAAGCTGGAGACCTTGT
TCAAGATCCTCGCCGCACTGGAACTCTCCCTCGATATCAGGCCCCGCACCGATCCTGCGGAAAACGCCACGCCTGGCTGG
AAGGAGGAGTGGTAA

Structures

Antitoxin

• AlphaFold DB: B0BSF9