Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1631815..1632341 Replicon chromosome
Accession NZ_CP037923
Organism Shigella flexneri 5a str. M90T

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag EKN05_RS08360 Protein ID WP_000323025.1
Coordinates 1632054..1632341 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag EKN05_RS08355 Protein ID WP_000534858.1
Coordinates 1631815..1632054 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKN05_RS08330 (EKN05_008360) 1627463..1627666 + 204 WP_000214712.1 putative selenium delivery protein YdfZ -
EKN05_RS08335 (EKN05_008365) 1627702..1629162 - 1461 WP_000527766.1 mannitol dehydrogenase family protein -
EKN05_RS08340 (EKN05_008370) 1629251..1630270 - 1020 Protein_1610 MHS family MFS transporter YdfJ -
EKN05_RS08345 (EKN05_008375) 1630332..1631488 + 1157 WP_136350417.1 IS3 family transposase -
EKN05_RS08350 (EKN05_008380) 1631485..1631790 - 306 WP_071818640.1 hypothetical protein -
EKN05_RS08355 (EKN05_008385) 1631815..1632054 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
EKN05_RS08360 (EKN05_008390) 1632054..1632341 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
EKN05_RS08365 (EKN05_008395) 1632413..1632568 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
EKN05_RS08370 (EKN05_008400) 1632786..1633037 + 252 WP_000980987.1 protein Rem -
EKN05_RS08375 (EKN05_008405) 1633104..1633382 + 279 WP_024258459.1 hypothetical protein -
EKN05_RS08380 (EKN05_008410) 1633384..1634434 + 1051 Protein_1618 DUF968 domain-containing protein -
EKN05_RS08385 (EKN05_008415) 1634447..1634821 + 375 WP_000904117.1 RusA family crossover junction endodeoxyribonuclease -
EKN05_RS25085 1634818..1635639 + 822 Protein_1620 antitermination protein -
EKN05_RS08410 (EKN05_008440) 1636222..1636500 + 279 WP_001017801.1 hypothetical protein -
EKN05_RS08415 (EKN05_008445) 1636559..1636667 + 109 Protein_1622 DUF3927 family protein -
EKN05_RS08420 (EKN05_008450) 1636948..1637283 - 336 WP_000871291.1 anti-adapter protein IraM -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1629251..1643278 14027


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T120372 WP_000323025.1 NZ_CP037923:1632054-1632341 [Shigella flexneri 5a str. M90T]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T120372 NZ_CP037923:1632054-1632341 [Shigella flexneri 5a str. M90T]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT120372 WP_000534858.1 NZ_CP037923:1631815-1632054 [Shigella flexneri 5a str. M90T]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT120372 NZ_CP037923:1631815-1632054 [Shigella flexneri 5a str. M90T]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References