120372

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1631815..1632341 Replicon chromosome
Accession NZ_CP037923
Organism Shigella flexneri 5a str. M90T

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag EKN05_RS08360 Protein ID WP_000323025.1
Coordinates 1632054..1632341 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag EKN05_RS08355 Protein ID WP_000534858.1
Coordinates 1631815..1632054 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKN05_RS08330 (EKN05_008360) 1627463..1627666 + 204 WP_000214712.1 putative selenium delivery protein YdfZ -
EKN05_RS08335 (EKN05_008365) 1627702..1629162 - 1461 WP_000527766.1 mannitol dehydrogenase family protein -
EKN05_RS08340 (EKN05_008370) 1629251..1630270 - 1020 Protein_1610 MHS family MFS transporter YdfJ -
EKN05_RS08345 (EKN05_008375) 1630332..1631488 + 1157 WP_136350417.1 IS3 family transposase -
EKN05_RS08350 (EKN05_008380) 1631485..1631790 - 306 WP_071818640.1 hypothetical protein -
EKN05_RS08355 (EKN05_008385) 1631815..1632054 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
EKN05_RS08360 (EKN05_008390) 1632054..1632341 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
EKN05_RS08365 (EKN05_008395) 1632413..1632568 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
EKN05_RS08370 (EKN05_008400) 1632786..1633037 + 252 WP_000980987.1 protein Rem -
EKN05_RS08375 (EKN05_008405) 1633104..1633382 + 279 WP_024258459.1 hypothetical protein -
EKN05_RS08380 (EKN05_008410) 1633384..1634434 + 1051 Protein_1618 DUF968 domain-containing protein -
EKN05_RS08385 (EKN05_008415) 1634447..1634821 + 375 WP_000904117.1 RusA family crossover junction endodeoxyribonuclease -
EKN05_RS25085 1634818..1635639 + 822 Protein_1620 antitermination protein -
EKN05_RS08410 (EKN05_008440) 1636222..1636500 + 279 WP_001017801.1 hypothetical protein -
EKN05_RS08415 (EKN05_008445) 1636559..1636667 + 109 Protein_1622 DUF3927 family protein -
EKN05_RS08420 (EKN05_008450) 1636948..1637283 - 336 WP_000871291.1 anti-adapter protein IraM -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1629251..1643278 14027


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T120372 WP_000323025.1 NZ_CP037923:1632054-1632341 [Shigella flexneri 5a str. M90T]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T120372 NZ_CP037923:1632054-1632341 [Shigella flexneri 5a str. M90T]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT120372 WP_000534858.1 NZ_CP037923:1631815-1632054 [Shigella flexneri 5a str. M90T]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT120372 NZ_CP037923:1631815-1632054 [Shigella flexneri 5a str. M90T]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References