113788

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 4566091..4566617 Replicon chromosome
Accession NZ_CP033510
Organism Shigella flexneri strain 2016AM-0877

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag EEL47_RS23330 Protein ID WP_000323025.1
Coordinates 4566091..4566378 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag EEL47_RS23335 Protein ID WP_000534858.1
Coordinates 4566378..4566617 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EEL47_RS23275 (4561149) 4561149..4561484 + 336 WP_000871291.1 anti-adapter protein IraM -
EEL47_RS23280 (4561765) 4561765..4561873 - 109 Protein_4442 DUF3927 family protein -
EEL47_RS23300 (4562794) 4562794..4563615 - 822 WP_000762882.1 antitermination protein -
EEL47_RS23305 (4563630) 4563630..4563986 - 357 WP_000904111.1 RusA family crossover junction endodeoxyribonuclease -
EEL47_RS23310 (4563999) 4563999..4565048 - 1050 WP_001265249.1 DUF968 domain-containing protein -
EEL47_RS23315 (4565050) 4565050..4565328 - 279 Protein_4446 hypothetical protein -
EEL47_RS23320 (4565395) 4565395..4565646 - 252 WP_000980987.1 protein Rem -
EEL47_RS23325 (4565864) 4565864..4566019 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
EEL47_RS23330 (4566091) 4566091..4566378 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
EEL47_RS23335 (4566378) 4566378..4566617 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
EEL47_RS23340 (4566642) 4566642..4566947 + 306 WP_071818640.1 hypothetical protein -
EEL47_RS23350 (4568162) 4568162..4569181 + 1020 Protein_4453 MHS family MFS transporter YdfJ -
EEL47_RS23355 (4569270) 4569270..4570730 + 1461 WP_000527804.1 mannitol dehydrogenase family protein -
EEL47_RS23360 (4570766) 4570766..4570969 - 204 WP_000214712.1 putative selenium delivery protein YdfZ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 4552808..4569181 16373


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T113788 WP_000323025.1 NZ_CP033510:c4566378-4566091 [Shigella flexneri]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T113788 NZ_CP033510:c4566378-4566091 [Shigella flexneri]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT113788 WP_000534858.1 NZ_CP033510:c4566617-4566378 [Shigella flexneri]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT113788 NZ_CP033510:c4566617-4566378 [Shigella flexneri]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References