Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 742855..743381 Replicon chromosome
Accession NZ_CP026776
Organism Shigella flexneri strain 98-3193

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag C1P84_RS04060 Protein ID WP_000323025.1
Coordinates 742855..743142 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag C1P84_RS04065 Protein ID WP_000534858.1
Coordinates 743142..743381 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P84_RS04005 (737913) 737913..738248 + 336 WP_000871291.1 anti-adapter protein IraM -
C1P84_RS04010 (738529) 738529..738637 - 109 Protein_757 DUF3927 family protein -
C1P84_RS04030 (739558) 739558..740379 - 822 WP_000762884.1 antitermination protein -
C1P84_RS04035 (740394) 740394..740750 - 357 WP_000904111.1 RusA family crossover junction endodeoxyribonuclease -
C1P84_RS04040 (740763) 740763..741812 - 1050 WP_001265249.1 DUF968 domain-containing protein -
C1P84_RS04045 (741814) 741814..742092 - 279 Protein_761 hypothetical protein -
C1P84_RS04050 (742195) 742195..742410 - 216 WP_000980986.1 hypothetical protein -
C1P84_RS04055 (742628) 742628..742783 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
C1P84_RS04060 (742855) 742855..743142 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
C1P84_RS04065 (743142) 743142..743381 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
C1P84_RS04070 (743406) 743406..743711 + 306 WP_071818640.1 hypothetical protein -
C1P84_RS04075 (743708) 743708..744586 - 879 Protein_767 IS3-like element IS600 family transposase -
C1P84_RS04085 (745358) 745358..745786 + 429 Protein_769 fimbrial protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 734716..753511 18795


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T95813 WP_000323025.1 NZ_CP026776:c743142-742855 [Shigella flexneri]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T95813 NZ_CP026776:c743142-742855 [Shigella flexneri]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT95813 WP_000534858.1 NZ_CP026776:c743381-743142 [Shigella flexneri]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT95813 NZ_CP026776:c743381-743142 [Shigella flexneri]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References