Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2320338..2320864 Replicon chromosome
Accession NZ_CP084879
Organism Shigella flexneri strain CMCC51574

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag LGV73_RS11930 Protein ID WP_000323025.1
Coordinates 2320577..2320864 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag LGV73_RS11925 Protein ID WP_000534858.1
Coordinates 2320338..2320577 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGV73_RS11905 (2317908) 2317908..2318336 - 429 Protein_2329 fimbrial protein -
LGV73_RS11910 (2318393) 2318393..2319090 + 698 WP_225620329.1 IS1 family transposase -
LGV73_RS11915 (2319106) 2319106..2320011 + 906 Protein_2331 IS3-like element IS600 family transposase -
LGV73_RS11920 (2320008) 2320008..2320313 - 306 WP_071818640.1 hypothetical protein -
LGV73_RS11925 (2320338) 2320338..2320577 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
LGV73_RS11930 (2320577) 2320577..2320864 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
LGV73_RS11935 (2320936) 2320936..2321091 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
LGV73_RS11940 (2321309) 2321309..2321560 + 252 WP_000980987.1 protein Rem -
LGV73_RS11945 (2321627) 2321627..2321905 + 279 Protein_2337 hypothetical protein -
LGV73_RS11950 (2321907) 2321907..2322956 + 1050 WP_001265249.1 DUF968 domain-containing protein -
LGV73_RS11955 (2322969) 2322969..2323325 + 357 WP_000904111.1 RusA family crossover junction endodeoxyribonuclease -
LGV73_RS11960 (2323340) 2323340..2324161 + 822 WP_000762884.1 antitermination protein -
LGV73_RS11975 (2325082) 2325082..2325190 + 109 Protein_2341 DUF3927 family protein -
LGV73_RS11980 (2325471) 2325471..2325806 - 336 WP_000871291.1 anti-adapter protein IraM -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2317908..2329002 11094


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T219781 WP_000323025.1 NZ_CP084879:2320577-2320864 [Shigella flexneri]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T219781 NZ_CP116710:c249473-249138 [Streptococcus agalactiae]
TTGGACTATAAGAAATATCAGATTATCTATGCTCCTGATGTTTTAGAGAAGCTAAAAGAAATTCGTGATTATATTTCCCA
AAACTATTCCTCAACATCAGGTCAGCGTAAAATGGAGCAAATCATTAATGACATAGAAAAACTTGAAGTTTTCCCAGAAG
TTGGTTTTGATGCCGATGAAAAATATGGTTCAGAAATCAGCAATTATCACAGTACTAGAGGTTACACCTTAAGTAAAGAT
TATATTGTCCTATACCATATCGAAGAGGAAGAGAATAGCGTTGTCATTGATTACTTGCTTCCTACTCGAAGCGACTATAT
GAAATTGTTTAAATAG

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT219781 WP_000534858.1 NZ_CP084879:2320338-2320577 [Shigella flexneri]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT219781 NZ_CP116710:c249750-249463 [Streptococcus agalactiae]
ATGGTTACAGTAGAAAAAAATAGAGCTGTCACATTCCAAGCTAACAAAGAATTGGTAAGCGAAGCAATGACAGTATTAAA
CAAAAAAAATTTAACCTTATCATCTGCTTTAAGATTATTCCTACAAAATGTCGTTGTCACAAACGAGGTTGACTTATTGA
CGGAAGAGGAGTTAGAGAAAGAAAAACTTTTCAAGCAATTTCAAGCCGAAATCAGCAAAAATATTGAGGATGTTCGTCAA
GGGAAGTTTTATACCTCTGAAGAAGTGAGGGCTGAACTTGGACTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References