Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2246338..2246864 Replicon chromosome
Accession NZ_LR213452
Organism Shigella flexneri strain AUSMDU00008355 isolate AUSMDU00008355

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag E0E33_RS11520 Protein ID WP_000323025.1
Coordinates 2246338..2246625 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag E0E33_RS11525 Protein ID WP_000534858.1
Coordinates 2246625..2246864 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E0E33_RS11465 2241396..2241731 + 336 WP_000871291.1 anti-adapter protein IraM -
E0E33_RS11470 2242012..2242120 - 109 Protein_2185 DUF3927 family protein -
E0E33_RS11490 2243041..2243862 - 822 WP_000762884.1 antitermination protein -
E0E33_RS11495 2243877..2244233 - 357 WP_000904111.1 RusA family crossover junction endodeoxyribonuclease -
E0E33_RS11500 2244246..2245295 - 1050 WP_001265249.1 DUF968 domain-containing protein -
E0E33_RS11505 2245297..2245575 - 279 Protein_2189 hypothetical protein -
E0E33_RS11510 2245678..2245893 - 216 WP_000980986.1 hypothetical protein -
E0E33_RS11515 2246111..2246266 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
E0E33_RS11520 2246338..2246625 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
E0E33_RS11525 2246625..2246864 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
E0E33_RS11530 2246889..2247194 + 306 WP_071818640.1 hypothetical protein -
E0E33_RS11535 2247191..2248069 - 879 Protein_2195 IS3-like element IS600 family transposase -
E0E33_RS11540 2248087..2248784 - 698 Protein_2196 IS1 family transposase -
E0E33_RS11545 2248841..2249269 + 429 Protein_2197 fimbrial protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2238199..2251882 13683


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T288249 WP_000323025.1 NZ_LR213452:c2246625-2246338 [Shigella flexneri]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT288249 WP_000534858.1 NZ_LR213452:c2246864-2246625 [Shigella flexneri]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References