Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2955014..2955540 Replicon chromosome
Accession NZ_CP037996
Organism Shigella flexneri strain FC906

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag CBL25_RS15080 Protein ID WP_000323025.1
Coordinates 2955014..2955301 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag CBL25_RS15085 Protein ID WP_000534858.1
Coordinates 2955301..2955540 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CBL25_RS15025 (2950072) 2950072..2950407 + 336 WP_000871291.1 anti-adapter protein IraM -
CBL25_RS15030 (2950688) 2950688..2950796 - 109 Protein_2884 DUF3927 family protein -
CBL25_RS15050 (2951717) 2951717..2952538 - 822 WP_000762882.1 antitermination protein -
CBL25_RS15055 (2952553) 2952553..2952909 - 357 WP_000904111.1 RusA family crossover junction endodeoxyribonuclease -
CBL25_RS15060 (2952922) 2952922..2953971 - 1050 WP_001265249.1 DUF968 domain-containing protein -
CBL25_RS15065 (2953973) 2953973..2954251 - 279 Protein_2888 hypothetical protein -
CBL25_RS15070 (2954318) 2954318..2954569 - 252 WP_000980987.1 protein Rem -
CBL25_RS15075 (2954787) 2954787..2954942 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
CBL25_RS15080 (2955014) 2955014..2955301 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
CBL25_RS15085 (2955301) 2955301..2955540 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
CBL25_RS15090 (2955565) 2955565..2955870 + 306 WP_071818640.1 hypothetical protein -
CBL25_RS15095 (2955867) 2955867..2957019 - 1153 Protein_2894 IS3 family transposase -
CBL25_RS15100 (2957081) 2957081..2958100 + 1020 Protein_2895 MHS family MFS transporter YdfJ -
CBL25_RS15105 (2958189) 2958189..2959649 + 1461 WP_000527804.1 mannitol dehydrogenase family protein -
CBL25_RS15110 (2959685) 2959685..2959888 - 204 WP_000214712.1 putative selenium delivery protein YdfZ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2946716..2958100 11384


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T120615 WP_000323025.1 NZ_CP037996:c2955301-2955014 [Shigella flexneri]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T120615 NZ_CP037996:c2955301-2955014 [Shigella flexneri]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT120615 WP_000534858.1 NZ_CP037996:c2955540-2955301 [Shigella flexneri]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT120615 NZ_CP037996:c2955540-2955301 [Shigella flexneri]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References