Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2589415..2589941 Replicon chromosome
Accession NZ_CP034060
Organism Shigella flexneri strain FDAARGOS_535

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag EGX88_RS14585 Protein ID WP_000323025.1
Coordinates 2589415..2589702 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag EGX88_RS14590 Protein ID WP_000534858.1
Coordinates 2589702..2589941 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGX88_RS14530 (2584473) 2584473..2584808 + 336 WP_000871291.1 anti-adapter protein IraM -
EGX88_RS14535 (2585089) 2585089..2585197 - 109 Protein_2491 DUF3927 family protein -
EGX88_RS14555 (2586118) 2586118..2586939 - 822 WP_000762884.1 antitermination protein -
EGX88_RS14560 (2586954) 2586954..2587310 - 357 WP_000904111.1 RusA family crossover junction endodeoxyribonuclease -
EGX88_RS14565 (2587323) 2587323..2588372 - 1050 WP_001265249.1 DUF968 domain-containing protein -
EGX88_RS14570 (2588374) 2588374..2588652 - 279 Protein_2495 hypothetical protein -
EGX88_RS14575 (2588755) 2588755..2588970 - 216 WP_000980986.1 hypothetical protein -
EGX88_RS14580 (2589188) 2589188..2589343 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
EGX88_RS14585 (2589415) 2589415..2589702 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
EGX88_RS14590 (2589702) 2589702..2589941 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
EGX88_RS14595 (2589966) 2589966..2590271 + 306 WP_071818640.1 hypothetical protein -
EGX88_RS14600 (2590268) 2590268..2591146 - 879 Protein_2501 IS3 family transposase -
EGX88_RS14610 (2591885) 2591885..2592346 + 462 Protein_2503 fimbrial protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2581276..2594959 13683


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T115085 WP_000323025.1 NZ_CP034060:c2589702-2589415 [Shigella flexneri]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T115085 NZ_CP034060:c2589702-2589415 [Shigella flexneri]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT115085 WP_000534858.1 NZ_CP034060:c2589941-2589702 [Shigella flexneri]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT115085 NZ_CP034060:c2589941-2589702 [Shigella flexneri]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References