74266

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1736456..1736982 Replicon chromosome
Accession NZ_CP020336
Organism Shigella flexneri 4c strain 1602

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag BS654_RS09665 Protein ID WP_000323025.1
Coordinates 1736456..1736743 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag BS654_RS09670 Protein ID WP_000534858.1
Coordinates 1736743..1736982 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BS654_RS09610 (1731514) 1731514..1731849 + 336 WP_000871291.1 anti-adapter protein IraM -
BS654_RS27615 (1732130) 1732130..1732238 - 109 Protein_1766 DUF3927 family protein -
BS654_RS09635 (1733159) 1733159..1733980 - 822 WP_000762882.1 antitermination protein -
BS654_RS09640 (1733995) 1733995..1734351 - 357 WP_000904111.1 RusA family crossover junction endodeoxyribonuclease -
BS654_RS09645 (1734364) 1734364..1735413 - 1050 WP_001265249.1 DUF968 domain-containing protein -
BS654_RS09650 (1735415) 1735415..1735693 - 279 Protein_1770 hypothetical protein -
BS654_RS09655 (1735760) 1735760..1736011 - 252 WP_000980987.1 protein Rem -
BS654_RS09660 (1736229) 1736229..1736384 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
BS654_RS09665 (1736456) 1736456..1736743 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
BS654_RS09670 (1736743) 1736743..1736982 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
BS654_RS09675 (1737007) 1737007..1737312 + 306 WP_071818640.1 hypothetical protein -
BS654_RS09680 (1737309) 1737309..1738465 - 1157 Protein_1776 IS3 family transposase -
BS654_RS09685 (1738527) 1738527..1739546 + 1020 Protein_1777 MHS family MFS transporter YdfJ -
BS654_RS09690 (1739635) 1739635..1741095 + 1461 WP_000527804.1 mannitol dehydrogenase family protein -
BS654_RS09695 (1741131) 1741131..1741334 - 204 WP_000214712.1 putative selenium delivery protein YdfZ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1725543..1739546 14003


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T74266 WP_000323025.1 NZ_CP020336:c1736743-1736456 [Shigella flexneri 4c]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T74266 NZ_CP020336:c1736743-1736456 [Shigella flexneri 4c]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT74266 WP_000534858.1 NZ_CP020336:c1736982-1736743 [Shigella flexneri 4c]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT74266 NZ_CP020336:c1736982-1736743 [Shigella flexneri 4c]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References