Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1736456..1736982 Replicon chromosome
Accession NZ_CP020336
Organism Shigella flexneri 4c strain 1602

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag BS654_RS09665 Protein ID WP_000323025.1
Coordinates 1736456..1736743 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag BS654_RS09670 Protein ID WP_000534858.1
Coordinates 1736743..1736982 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BS654_RS09610 (1731514) 1731514..1731849 + 336 WP_000871291.1 anti-adapter protein IraM -
BS654_RS27615 (1732130) 1732130..1732238 - 109 Protein_1766 DUF3927 family protein -
BS654_RS09635 (1733159) 1733159..1733980 - 822 WP_000762882.1 antitermination protein -
BS654_RS09640 (1733995) 1733995..1734351 - 357 WP_000904111.1 RusA family crossover junction endodeoxyribonuclease -
BS654_RS09645 (1734364) 1734364..1735413 - 1050 WP_001265249.1 DUF968 domain-containing protein -
BS654_RS09650 (1735415) 1735415..1735693 - 279 Protein_1770 hypothetical protein -
BS654_RS09655 (1735760) 1735760..1736011 - 252 WP_000980987.1 protein Rem -
BS654_RS09660 (1736229) 1736229..1736384 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
BS654_RS09665 (1736456) 1736456..1736743 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
BS654_RS09670 (1736743) 1736743..1736982 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
BS654_RS09675 (1737007) 1737007..1737312 + 306 WP_071818640.1 hypothetical protein -
BS654_RS09680 (1737309) 1737309..1738465 - 1157 Protein_1776 IS3 family transposase -
BS654_RS09685 (1738527) 1738527..1739546 + 1020 Protein_1777 MHS family MFS transporter YdfJ -
BS654_RS09690 (1739635) 1739635..1741095 + 1461 WP_000527804.1 mannitol dehydrogenase family protein -
BS654_RS09695 (1741131) 1741131..1741334 - 204 WP_000214712.1 putative selenium delivery protein YdfZ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1725543..1739546 14003


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T74266 WP_000323025.1 NZ_CP020336:c1736743-1736456 [Shigella flexneri 4c]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T74266 NZ_CP020336:c1736743-1736456 [Shigella flexneri 4c]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT74266 WP_000534858.1 NZ_CP020336:c1736982-1736743 [Shigella flexneri 4c]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT74266 NZ_CP020336:c1736982-1736743 [Shigella flexneri 4c]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References